##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062037_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1623871
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247730269214735	32.0	32.0	32.0	32.0	32.0
2	30.94032161421689	32.0	32.0	32.0	32.0	32.0
3	30.948051908064127	32.0	32.0	32.0	32.0	32.0
4	30.939125706413872	32.0	32.0	32.0	32.0	32.0
5	30.93255991393405	32.0	32.0	32.0	32.0	32.0
6	34.406065506434935	36.0	36.0	36.0	32.0	36.0
7	34.391432570690654	36.0	36.0	36.0	32.0	36.0
8	34.37183495487018	36.0	36.0	36.0	32.0	36.0
9	34.48539385209786	36.0	36.0	36.0	32.0	36.0
10	34.291151205976334	36.0	36.0	36.0	32.0	36.0
11	34.419370750509124	36.0	36.0	36.0	32.0	36.0
12	34.33291191233786	36.0	36.0	36.0	32.0	36.0
13	34.38123964280414	36.0	36.0	36.0	32.0	36.0
14	34.326019123440226	36.0	36.0	36.0	32.0	36.0
15	34.28060233848625	36.0	36.0	36.0	32.0	36.0
16	34.28327742782524	36.0	36.0	36.0	32.0	36.0
17	34.23344342007462	36.0	36.0	36.0	32.0	36.0
18	34.22922325726613	36.0	36.0	36.0	32.0	36.0
19	34.2299979493445	36.0	36.0	36.0	32.0	36.0
20	34.19798740170863	36.0	36.0	36.0	32.0	36.0
21	34.177298566203845	36.0	36.0	36.0	32.0	36.0
22	34.14600482427483	36.0	36.0	36.0	32.0	36.0
23	34.12805265935533	36.0	36.0	36.0	32.0	36.0
24	34.09973637068462	36.0	36.0	36.0	32.0	36.0
25	33.64224313384499	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	24.0
5	129.0
6	340.0
7	60.0
8	297.0
9	221.0
10	121.0
11	35.0
12	74.0
13	75.0
14	248.0
15	415.0
16	693.0
17	988.0
18	1350.0
19	1726.0
20	2287.0
21	2936.0
22	3780.0
23	5175.0
24	7083.0
25	9882.0
26	14265.0
27	19160.0
28	26346.0
29	36722.0
30	51660.0
31	75732.0
32	116003.0
33	177298.0
34	404617.0
35	664127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72178858334026	17.35234980955812	12.015974332683644	25.909887274417976
2	16.843107418147646	19.009230424375364	37.344145371332296	26.803516786144694
3	19.57963150786921	22.043019691956392	27.929545693874047	30.447803106300352
4	13.3182484170384	15.326874410681304	34.47120983779609	36.8836673344842
5	15.425770789556847	35.61405356855744	33.18333053040622	15.776845111479496
6	35.41147132169576	34.109438536103525	16.28483769572856	14.194252446472156
7	31.25053169913165	29.559697637344822	19.617447868826588	19.57232279469694
8	28.351628299227798	33.116621314549704	18.668509520245703	19.86324086597679
9	26.779011669774004	14.96170084736567	18.51146261825141	39.74782486460892
10	16.77206487169084	26.581127226403485	30.573207562526743	26.073600339378935
11	36.95774835490716	21.365888010971602	21.675080982782255	20.001282651338983
12	24.49961029415392	23.392710034185587	28.28123689675755	23.826442774902944
13	29.4510923215324	19.52959050351426	24.78837060316767	26.23094657178567
14	23.581326355973584	19.235786753209602	24.820099530951758	32.36278735986506
15	25.32058476041546	26.832185994657536	21.820297411890063	26.026931833036944
16	25.888008463917373	25.86254519268236	23.19685513186645	25.052591211533816
17	24.247872512279066	25.89104155337124	24.83022641821211	25.030859516137582
18	24.769446542237567	25.368679006827676	25.489326942342373	24.37254750859239
19	25.654310196568904	25.01964826068973	24.94691117013637	24.379130372604994
20	25.789789338144427	24.5037023156963	24.832975110995505	24.87353323516377
21	26.452867148556948	24.27178661222749	24.48437801451095	24.790968224704613
22	25.88839778324364	24.103773294127077	24.964954783348436	25.042874139280848
23	24.903854450429574	24.340971563104763	25.04603892662122	25.70913505984444
24	24.74070229658837	24.87143752662012	25.006980558604948	25.380879618186558
25	24.955529449181533	24.45035878458256	25.071097408688985	25.523014357546923
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	68.0
2	232.5
3	397.0
4	397.0
5	397.0
6	808.0
7	1219.0
8	1219.0
9	1219.0
10	1568.5
11	1918.0
12	1918.0
13	1918.0
14	2737.5
15	3557.0
16	3557.0
17	3557.0
18	6298.0
19	9039.0
20	9039.0
21	9039.0
22	14144.5
23	19250.0
24	19250.0
25	19250.0
26	29035.5
27	38821.0
28	38821.0
29	38821.0
30	50515.0
31	62209.0
32	62209.0
33	62209.0
34	77052.0
35	91895.0
36	91895.0
37	91895.0
38	110224.0
39	128553.0
40	128553.0
41	128553.0
42	149448.5
43	170344.0
44	170344.0
45	170344.0
46	189905.5
47	209467.0
48	209467.0
49	209467.0
50	218126.0
51	226785.0
52	226785.0
53	226785.0
54	215627.0
55	204469.0
56	204469.0
57	204469.0
58	187901.0
59	171333.0
60	171333.0
61	171333.0
62	148553.0
63	125773.0
64	125773.0
65	125773.0
66	102746.5
67	79720.0
68	79720.0
69	79720.0
70	60186.5
71	40653.0
72	40653.0
73	40653.0
74	30631.0
75	20609.0
76	20609.0
77	20609.0
78	15683.0
79	10757.0
80	10757.0
81	10757.0
82	7460.0
83	4163.0
84	4163.0
85	4163.0
86	2906.0
87	1649.0
88	1649.0
89	1649.0
90	1059.0
91	469.0
92	469.0
93	469.0
94	322.5
95	176.0
96	176.0
97	176.0
98	377.0
99	578.0
100	578.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.8529994069726E-4
2	1.231624925871575E-4
3	6.158124629357873E-4
4	0.0107151368550827
5	0.034978147894752724
6	0.06305919620462463
7	0.10548867490090039
8	0.10770559976746921
9	0.11718911169668034
10	0.12839689852211167
11	0.1373261792346806
12	0.1314759608367906
13	0.12624155490183642
14	0.1147258618449372
15	0.13221493579231355
16	0.11866706160772622
17	0.12347039881862537
18	0.1105999183432674
19	0.0979141816067902
20	0.09286451941071673
21	0.09298768190330389
22	0.10351807501950586
23	0.0819646388167533
24	0.09286451941071673
25	0.0892312258793956
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1623871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.619335426539095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.16806293768225	35.51860714073044
2	15.629403902747693	15.823000773400892
3	6.016952750010941	9.13722448495322
4	2.832056610255382	5.7342729400585775
5	1.5619635362936757	3.9532778083836364
6	0.918512779193453	2.789670387813361
7	0.6378154444764732	2.26000557469272
8	0.4144674400766059	1.6784053098093363
9	0.2975050354610602	1.355355646297903
>10	1.3994904960611374	12.189787212215524
>50	0.06950710113607791	2.4175164069082022
>100	0.0490188816417036	5.000592629654871
>500	0.004389559504415857	1.5382793017166547
>1k	8.53525459191972E-4	0.6040043833647307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2502	0.154076278226534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.231624925871575E-4
2	0.0	0.0	0.0	0.0	1.231624925871575E-4
3	0.0	0.0	0.0	0.0	1.231624925871575E-4
4	0.0	0.0	0.0	0.0	1.231624925871575E-4
5	0.0	0.0	0.0	0.0	1.231624925871575E-4
6	0.0	0.0	0.0	0.0	1.847437388807362E-4
7	0.0	0.0	0.0	0.0	1.847437388807362E-4
8	0.0	0.0	0.0	0.0	2.46324985174315E-4
9	0.0	0.0	0.0	0.0	2.46324985174315E-4
10	0.0	0.0	0.0	0.0	2.46324985174315E-4
11	0.0	0.0	0.0	0.0	2.46324985174315E-4
12	0.0	0.0	0.0	0.0	6.773937092293661E-4
13	0.0	0.0	0.0	0.0	6.773937092293661E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	600	0.0	14.245214	1
CGCGTAA	100	1.0053554E-8	13.301683	10
CGCGCCA	65	5.446302E-5	13.155511	10
CGAACGA	65	5.4526085E-5	13.153889	16
CTTATAG	125	1.03682396E-10	12.918051	3
GCGTAAC	105	1.9901563E-8	12.66827	11
TATACCG	75	1.477222E-5	12.66788	5
TACACCG	60	4.088892E-4	12.667879	5
GTATCAA	1675	0.0	12.473423	1
TCGCGTA	115	5.313268E-9	12.392872	9
TCTTATA	125	1.8371793E-8	11.397577	2
GGCCTAA	75	2.080401E-4	11.396172	1
CCGCCCA	85	5.3160988E-5	11.177886	9
CAAGACG	170	1.0913936E-11	11.175129	4
CCTAACC	85	5.3311473E-5	11.174784	3
AACCGCT	70	0.0014902717	10.858183	7
TCTATAC	140	8.36917E-9	10.855504	3
GGTTTAA	70	0.0014956475	10.853497	1
GTCTAGC	115	8.853749E-7	10.735524	1
GGACCGT	80	3.7675287E-4	10.688523	6
>>END_MODULE