FastQCFastQC Report
Thu 2 Feb 2017
SRR4062037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623871
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.026.7632181
GTATCAA17000.026.0180451
AATCCCG1105.2586984E-921.99921419
ACCGTCG3950.021.1638018
CGCAATA2000.019.79990236
TACCGTC4200.019.380267
CGTCGTA4050.019.01166710
ATACCGT4100.018.7798186
CCGTCGT4200.018.332689
TCTAGCG2350.017.78824228
TCAACGC24150.017.4900594
GTCGTAG4850.017.236511
ATCAACG24750.017.1549433
AACGAAT1307.3209776E-716.92299531
TATCAAC25900.016.6485672
TATGCGA800.001989221216.4994112
CAACGCA25500.016.4778445
GTATAGA2353.6379788E-1215.91873551
CCTAATA2500.015.8399242
AACGCAG26700.015.73726756
Produced by FastQC (version 0.11.5)