##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1623871
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.165520537037732	32.0	32.0	32.0	32.0	32.0
2	31.172891812219074	32.0	32.0	32.0	32.0	32.0
3	31.405052494933404	32.0	32.0	32.0	32.0	32.0
4	31.53495012842769	32.0	32.0	32.0	32.0	32.0
5	31.461467690475413	32.0	32.0	32.0	32.0	32.0
6	35.08701430101283	36.0	36.0	36.0	36.0	36.0
7	35.10265532175893	36.0	36.0	36.0	36.0	36.0
8	35.029946344260104	36.0	36.0	36.0	36.0	36.0
9	35.16590665145199	36.0	36.0	36.0	36.0	36.0
10	34.9884676800066	36.0	36.0	36.0	36.0	36.0
11	35.17050430730027	36.0	36.0	36.0	36.0	36.0
12	35.068879855604294	36.0	36.0	36.0	36.0	36.0
13	35.11973056973122	36.0	36.0	36.0	36.0	36.0
14	35.07985794438105	36.0	36.0	36.0	36.0	36.0
15	35.0352780485642	36.0	36.0	36.0	36.0	36.0
16	35.058334682988985	36.0	36.0	36.0	36.0	36.0
17	35.034359872181966	36.0	36.0	36.0	36.0	36.0
18	35.02859094102918	36.0	36.0	36.0	36.0	36.0
19	35.016542570191845	36.0	36.0	36.0	36.0	36.0
20	35.02425069479041	36.0	36.0	36.0	36.0	36.0
21	35.01518224046122	36.0	36.0	36.0	36.0	36.0
22	34.994272328282236	36.0	36.0	36.0	36.0	36.0
23	34.960264085016604	36.0	36.0	36.0	32.0	36.0
24	34.94291110562354	36.0	36.0	36.0	32.0	36.0
25	34.92387326333188	36.0	36.0	36.0	32.0	36.0
26	34.87890910053816	36.0	36.0	36.0	32.0	36.0
27	34.85490596235785	36.0	36.0	36.0	32.0	36.0
28	34.83918488599156	36.0	36.0	36.0	32.0	36.0
29	34.822748235543344	36.0	36.0	36.0	32.0	36.0
30	34.80042133888714	36.0	36.0	36.0	32.0	36.0
31	34.801945474732904	36.0	36.0	36.0	32.0	36.0
32	34.76912205464597	36.0	36.0	36.0	32.0	36.0
33	34.7499031634902	36.0	36.0	36.0	32.0	36.0
34	34.734079862255065	36.0	36.0	36.0	32.0	36.0
35	34.72174021212276	36.0	36.0	36.0	32.0	36.0
36	34.69410193297374	36.0	36.0	36.0	32.0	36.0
37	34.68735139675504	36.0	36.0	36.0	32.0	36.0
38	34.67307871130158	36.0	36.0	36.0	32.0	36.0
39	34.65517889044142	36.0	36.0	36.0	32.0	36.0
40	34.64025282796478	36.0	36.0	36.0	32.0	36.0
41	34.617083499859284	36.0	36.0	36.0	32.0	36.0
42	34.58083739410335	36.0	36.0	36.0	32.0	36.0
43	34.57923936076203	36.0	36.0	36.0	32.0	36.0
44	34.536759385443794	36.0	36.0	36.0	32.0	36.0
45	34.5172436726809	36.0	36.0	36.0	32.0	36.0
46	34.50134401070036	36.0	36.0	36.0	32.0	36.0
47	34.48016252522522	36.0	36.0	36.0	32.0	36.0
48	34.45306185035634	36.0	36.0	36.0	32.0	36.0
49	34.44362267692446	36.0	36.0	36.0	32.0	36.0
50	33.953503079986035	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	23.0
22	78.0
23	236.0
24	707.0
25	1703.0
26	3612.0
27	7425.0
28	13111.0
29	22313.0
30	35111.0
31	53849.0
32	84931.0
33	150493.0
34	335530.0
35	914734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37942016219196	17.871053385364533	12.536890509644971	26.212635942798535
2	16.328026224952996	19.56556145872914	36.75619550966039	27.350216806657485
3	18.953141511502167	22.53736141114918	28.127347789352335	30.382149287996313
4	13.095990999285043	15.8976913806577	34.550158233012354	36.456159387044906
5	15.290130804725251	36.00236718310752	32.739423266995956	15.96807874517126
6	35.67039131947545	34.248167181385156	16.10977513524831	13.971666363891085
7	31.451820988243522	29.60949484287853	19.50062535755611	19.438058811321834
8	28.506697884253125	33.162794335264316	18.481455731397382	19.84905204908518
9	26.9533109464976	14.741318737756878	18.26961624414747	40.03575407159805
10	16.77836478390217	26.67496371325062	30.4872739275472	26.059397575300007
11	37.31035285438314	21.240172402857123	21.505772318121327	19.94370242463841
12	24.54974844045398	23.31929280053206	28.185199554151502	23.94575920486246
13	29.67353933902385	19.369334140458204	24.73152116147157	26.225605359046376
14	23.68962805543051	19.07503736442119	24.66267332811535	32.57266125203295
15	25.42757398832789	26.76351754542079	21.726664248576398	26.082244217674926
16	26.065408307730774	25.759532373768444	23.133815024808158	25.04124429369263
17	24.56063320300689	25.822617683301196	24.532367410958138	25.084381702733776
18	25.04516987197251	25.342176405746763	25.21741272392494	24.39524099835578
19	25.929091658142795	24.970025328366603	24.68884535779012	24.412037655700484
20	26.09358055451722	24.422257296335957	24.546528863580242	24.937633285566577
21	26.661662164051208	24.272802457830704	24.205186249400352	24.860349128717736
22	26.116914459338208	24.051048389927526	24.772780596488268	25.059256554245994
23	25.160203710888183	24.248554379353028	24.861596063724313	25.729645846034472
24	24.9770299263726	24.782061419390438	24.806755788089063	25.4341528661479
25	25.348149790315727	24.147253166817542	25.012162303632678	25.492434739234053
26	25.060503760778293	25.01320921800198	25.108414117913668	24.81787290330606
27	25.485477249970135	24.687936073543014	24.692924169851455	25.133662506635403
28	25.353405035579136	24.506156683673627	24.97350424455536	25.16693403619188
29	25.072604602355863	24.784403127355493	24.863966440539357	25.27902582974929
30	25.195461162716796	24.94346775445053	24.825969361739865	25.035101721092808
31	25.49831759309893	24.566650901799658	24.50888744377608	25.426144061325335
32	25.460322934243102	24.640301503824222	24.508024090746737	25.391351471185942
33	25.21558081347695	24.53769361937447	24.726378790407356	25.520346776741228
34	25.550017304468742	24.622411565543292	24.785972665097564	25.041598464890402
35	25.628622523505605	24.487088852049553	25.033007608992847	24.851281015451995
36	24.877237407520077	24.970348538610946	24.73818652524285	25.41422752862613
37	25.712227114573388	24.609107984111834	24.57776272438957	25.10090217692521
38	25.15760180457684	24.61433204977489	24.8213681998139	25.406697945834367
39	25.662170192719223	24.587142404079156	24.451108928389985	25.29957847481164
40	25.75387900987026	24.709705468671096	24.562341274044442	24.974074247414197
41	24.97973958282015	24.91421653151511	24.959109674749484	25.146934210915255
42	25.81289409944716	24.79835841549159	24.615638531171076	24.773108953890173
43	25.5267890134253	24.250646631358542	24.89253602660426	25.3300283286119
44	25.0743165555793	24.673096452327478	24.685536430970128	25.567050561123096
45	25.36892466688549	24.83974724126315	24.569770069852897	25.221558021998458
46	25.205731349313144	24.736227253776693	24.492422595562005	25.565618801348162
47	25.19453791241545	24.733965247978876	24.905779358659185	25.16571748094649
48	25.526140437958723	25.033470747121267	24.22407602878669	25.216312786133322
49	25.24197526454546	24.895385765566814	24.552060169119414	25.31057880076831
50	25.35416050842447	24.99187112030742	24.38651591289782	25.267452458370283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	6.5
2	6.0
3	30.5
4	55.0
5	67.5
6	80.0
7	85.5
8	91.0
9	110.5
10	130.0
11	226.0
12	322.0
13	621.5
14	921.0
15	1236.5
16	1552.0
17	1629.0
18	1706.0
19	1936.0
20	2166.0
21	2614.0
22	3062.0
23	3968.5
24	4875.0
25	6541.0
26	8207.0
27	10631.5
28	13056.0
29	15368.5
30	17681.0
31	20426.5
32	23172.0
33	27028.5
34	30885.0
35	36038.0
36	41191.0
37	46962.5
38	52734.0
39	59802.5
40	66871.0
41	73494.0
42	80117.0
43	84809.0
44	89501.0
45	97166.5
46	104832.0
47	111299.0
48	117766.0
49	123240.5
50	128715.0
51	127726.5
52	126738.0
53	127384.0
54	128030.0
55	126639.0
56	125248.0
57	119920.0
58	114592.0
59	106197.0
60	97802.0
61	86477.5
62	75153.0
63	64732.0
64	54311.0
65	45494.0
66	36677.0
67	31713.5
68	26750.0
69	22500.0
70	18250.0
71	14434.0
72	10618.0
73	9310.5
74	8003.0
75	6129.0
76	4255.0
77	3688.0
78	3121.0
79	2613.0
80	2105.0
81	1665.0
82	1225.0
83	931.5
84	638.0
85	505.0
86	372.0
87	255.5
88	139.0
89	95.0
90	51.0
91	41.5
92	32.0
93	25.5
94	19.0
95	18.0
96	17.0
97	16.0
98	15.0
99	12.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029928485698679265
2	0.004557012225724827
3	1.847437388807362E-4
4	0.0
5	0.0
6	3.694874777614724E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	6.158124629357874E-5
13	0.0
14	0.0
15	0.0
16	6.158124629357873E-4
17	0.0
18	6.158124629357874E-5
19	0.0
20	4.9264997034863E-4
21	0.0
22	0.0
23	6.158124629357874E-5
24	0.001170043679577996
25	6.158124629357874E-5
26	3.0790623146789367E-4
27	3.0790623146789367E-4
28	0.0016011124036330471
29	4.3106872405505116E-4
30	0.001170043679577996
31	4.3106872405505116E-4
32	6.773937092293661E-4
33	1.231624925871575E-4
34	8.005562018165236E-4
35	1.847437388807362E-4
36	3.0790623146789367E-4
37	0.0012932061721651535
38	0.0
39	3.694874777614724E-4
40	1.847437388807362E-4
41	9.237186944036811E-4
42	0.004557012225724827
43	0.0043722684868440906
44	0.004557012225724827
45	0.0018474373888073621
46	0.0018474373888073621
47	0.001416368664752311
48	0.004002781009082618
49	0.0029558998220917793
50	0.001909018635100941
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1623871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.14576016574587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.20999689786309	36.611536594737295
2	15.757165953517655	16.43338793407976
3	6.065863278800158	9.48927155203554
4	2.8215097275944157	5.885190782418294
5	1.4805798660547749	3.8602981300762207
6	0.9418667462433497	2.9468614474618278
7	0.5764701194664628	2.1042330814691925
8	0.4156705629172087	1.7340365985473074
9	0.2885828886130222	1.3543536687797528
>10	1.3364838302296644	11.814302548570991
>50	0.06350457924755663	2.237294653711068
>100	0.03838416807038457	3.9973424208052735
>500	0.003208402949126007	1.1129474491800848
>1k	7.129784331391126E-4	0.418943138127418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.158124629357874E-5
2	0.0	0.0	0.0	0.0	6.158124629357874E-5
3	0.0	0.0	0.0	0.0	6.158124629357874E-5
4	0.0	0.0	0.0	0.0	6.158124629357874E-5
5	0.0	0.0	0.0	0.0	6.158124629357874E-5
6	0.0	0.0	0.0	0.0	1.231624925871575E-4
7	0.0	0.0	0.0	0.0	1.231624925871575E-4
8	0.0	0.0	0.0	0.0	1.231624925871575E-4
9	0.0	0.0	0.0	0.0	1.231624925871575E-4
10	0.0	0.0	0.0	0.0	1.231624925871575E-4
11	0.0	0.0	0.0	0.0	1.231624925871575E-4
12	0.0	0.0	0.0	0.0	7.389749555229448E-4
13	0.0	0.0	0.0	0.0	9.8529994069726E-4
14	0.0	0.0	0.0	0.0	9.8529994069726E-4
15	0.0	0.0	0.0	0.0	0.0012316249258715747
16	0.0	0.0	0.0	0.0	0.0013547874184587322
17	0.0	0.0	0.0	0.0	0.0013547874184587322
18	0.0	0.0	0.0	6.158124629357874E-5	0.001416368664752311
19	0.0	0.0	0.0	6.158124629357874E-5	0.001416368664752311
20	0.0	0.0	0.0	2.46324985174315E-4	0.0016011124036330471
21	0.0	0.0	0.0	3.0790623146789367E-4	0.001662693649926626
22	0.0	0.0	0.0	3.0790623146789367E-4	0.001662693649926626
23	0.0	0.0	0.0	4.3106872405505116E-4	0.0017858561425137835
24	0.0	0.0	0.0	6.158124629357873E-4	0.0017858561425137835
25	0.0	0.0	0.0	0.0010468811869908386	0.0017858561425137835
26	0.0	0.0	0.0	0.0014779499110458897	0.001909018635100941
27	0.0	0.0	0.0	0.0017242748962202046	0.001909018635100941
28	0.0	0.0	0.0	0.0022785061128624134	0.001909018635100941
29	0.0	0.0	0.0	0.0030174810683853584	0.00197059988139452
30	0.0	0.0	0.0	0.004249105994256933	0.00197059988139452
31	0.0	0.0	0.0	0.00732816830893587	0.00197059988139452
32	0.0	0.0	0.0	0.009976161899559756	0.00197059988139452
33	0.0	0.0	0.0	0.014040524154935952	0.00197059988139452
34	0.0	0.0	0.0	0.017858561425137833	0.00197059988139452
35	0.0	0.0	0.0	0.021861342434220454	0.00197059988139452
36	0.0	0.0	0.0	0.027095748369174645	0.00197059988139452
37	0.0	0.0	0.0	0.03651767905209219	0.00197059988139452
38	0.0	0.0	0.0	0.047910209616404256	0.00197059988139452
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	26.763218	1
GTATCAA	1700	0.0	26.018045	1
AATCCCG	110	5.2586984E-9	21.999214	19
ACCGTCG	395	0.0	21.163801	8
CGCAATA	200	0.0	19.799902	36
TACCGTC	420	0.0	19.38026	7
CGTCGTA	405	0.0	19.011667	10
ATACCGT	410	0.0	18.779818	6
CCGTCGT	420	0.0	18.33268	9
TCTAGCG	235	0.0	17.788242	28
TCAACGC	2415	0.0	17.490059	4
GTCGTAG	485	0.0	17.2365	11
ATCAACG	2475	0.0	17.154943	3
AACGAAT	130	7.3209776E-7	16.922995	31
TATCAAC	2590	0.0	16.648567	2
TATGCGA	80	0.0019892212	16.49941	12
CAACGCA	2550	0.0	16.477844	5
GTATAGA	235	3.6379788E-12	15.9187355	1
CCTAATA	250	0.0	15.839924	2
AACGCAG	2670	0.0	15.7372675	6
>>END_MODULE