FastQCFastQC Report
Thu 2 Feb 2017
SRR4062036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751674
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23480.3123694580363296No Hit
CCATAGGGTCTTCTCGTCTTATTAT19380.25782453563645946No Hit
TATCAACGCAGAGTACTTTTTTTTT13640.18146164427664122No Hit
GTATTAGAGGCACTGCCTGCCCAGT11690.15551954703767856No Hit
CTCTAATACTTGTAATGCTAGAGGT9560.12718279466896554No Hit
CCATTGGGATGTCCTGATCCAACAT9500.12638457629238206No Hit
GTCAGGATACCGCGGCCGTTAAACT9270.12332473918214544No Hit
GTTATATAATTTAAGCTCCATAGGG9230.12279259359775647No Hit
GATTAAACCTTGTACCTTTTGCATA8960.11920061090313087No Hit
GGTATCAACGCAGAGTACTTTTTTT8910.11853542892264467No Hit
CCTATAACTTCTCTGTTAACCCAAC8860.11787024694215843No Hit
ATAAATAATCCACCTATAACTTCTC8860.11787024694215843No Hit
GACTATAGGCAATAATCACACTATA8600.11441130064363009No Hit
ACCTATAACTTCTCTGTTAACCCAA8500.11308093668265765No Hit
GCCTAAAGGAAAGATCCAAAAAGAT8410.11188360911778245No Hit
GTTCATGCTAGTCCCTAATTAAGGA8400.1117505727216852No Hit
CACTATAAATAATCCACCTATAACT8380.11148449992949071No Hit
CTTCTACACCATTGGGATGTCCTGA8380.11148449992949071No Hit
CTATAGAACTAGTACCGCAAGGGAA8380.11148449992949071No Hit
CTGTTAGTATGAGTAACAAGAATTC8000.10642911687779542No Hit
ATCTATAACTTTATAGATGCAACAC7910.10523178931292022No Hit
ATCCTGACCGTGCAAAGGTAGCATA7610.10124069743000291No Hit
CTGTTAACCCAACACCGGAATGCCT7570.10070855184561392No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7560.10057551544951668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT456.7857374E-414.7693821
TGCGTCA400.00526996414.25235110
GTCCGAC602.5623913E-514.25140112
CGTACTC759.657324E-713.9328453
TACACAG551.9563938E-413.8195395
TAGGACC1101.8553692E-1013.8176974
GTTAAGC903.630521E-813.7217415
GCGTTCA852.6895032E-713.41308313
GGTATCA5200.012.9637851
AACGTAA1102.730303E-912.95668110
CTGTAGG2550.012.65947151
AGGACCT1301.9645086E-1012.4242975
TGTAGGA2950.012.2335972
GTACTAG701.0952455E-412.2073471
GTTACAA1251.4060788E-912.15957315
CGTCCGA550.00306058812.09370911
CTATCCT1103.803507E-812.0904854
CCTACAT550.00306670812.0904853
CGACATG550.00306670812.09048515
TACACCT1353.6925485E-1011.9641395
Produced by FastQC (version 0.11.5)