##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062036_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	751674
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283649560846857	32.0	32.0	32.0	32.0	32.0
2	30.907498463429626	32.0	32.0	32.0	32.0	32.0
3	30.94438945606739	32.0	32.0	32.0	32.0	32.0
4	30.94032785489454	32.0	32.0	32.0	32.0	32.0
5	30.920796781583505	32.0	32.0	32.0	32.0	32.0
6	34.547933013513834	36.0	36.0	36.0	32.0	36.0
7	34.4636318403989	36.0	36.0	36.0	32.0	36.0
8	34.42072627229357	36.0	36.0	36.0	32.0	36.0
9	34.516461923653075	36.0	36.0	36.0	32.0	36.0
10	34.28491074588186	36.0	36.0	36.0	32.0	36.0
11	34.537303405465664	36.0	36.0	36.0	32.0	36.0
12	34.392746057466404	36.0	36.0	36.0	32.0	36.0
13	34.47198253498192	36.0	36.0	36.0	32.0	36.0
14	34.389796640564924	36.0	36.0	36.0	32.0	36.0
15	34.35217660847655	36.0	36.0	36.0	32.0	36.0
16	34.362110436173126	36.0	36.0	36.0	32.0	36.0
17	34.315998690921866	36.0	36.0	36.0	32.0	36.0
18	34.31641243411372	36.0	36.0	36.0	32.0	36.0
19	34.31166569550098	36.0	36.0	36.0	32.0	36.0
20	34.28303892378877	36.0	36.0	36.0	32.0	36.0
21	34.27799551401272	36.0	36.0	36.0	32.0	36.0
22	34.24665346945618	36.0	36.0	36.0	32.0	36.0
23	34.241209620127876	36.0	36.0	36.0	32.0	36.0
24	34.20312795174504	36.0	36.0	36.0	32.0	36.0
25	33.879866803960226	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	14.0
5	50.0
6	148.0
7	22.0
8	125.0
9	101.0
10	68.0
11	17.0
12	30.0
13	28.0
14	148.0
15	200.0
16	323.0
17	414.0
18	612.0
19	818.0
20	1113.0
21	1511.0
22	2040.0
23	2724.0
24	3739.0
25	5191.0
26	7119.0
27	9336.0
28	12740.0
29	17024.0
30	22783.0
31	31297.0
32	45613.0
33	66756.0
34	156303.0
35	363266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.273940090571905	21.516813273945413	12.662505254979592	24.546741380503097
2	15.31463814717304	21.083769138973995	39.9548739944098	23.646718719443165
3	16.693207533615105	26.73882589590393	30.71636394113914	25.851602629341823
4	10.925124433380743	17.919471213014248	40.762792257123735	30.392612096481276
5	11.706902907362826	39.5572749279048	36.188396365896274	12.547425798836095
6	28.107254008780313	41.56152742317226	18.301017270435263	12.03020129761217
7	26.798658156069926	33.660626415787846	22.258282317251005	17.28243311089123
8	25.377960557858064	35.42454639405918	20.202111678155532	18.99538136992723
9	25.61222981243199	15.413527325421105	21.719557657550865	37.254685204596036
10	14.684565760922414	27.34163017981237	33.90500141862488	24.06880264064034
11	32.37519616123454	24.817826493556318	24.858324296816924	17.94865304839221
12	21.936095759120946	27.38222259519899	30.361774143918414	20.31990750176165
13	25.831076680035647	22.240633612301412	28.25560811089814	23.672681596764804
14	20.862417309610663	23.013066646422754	27.463397807531003	28.661118236435577
15	23.74599537761674	29.909617088164968	23.68152178980811	22.66286574441018
16	23.029076868366268	29.14506202026364	25.62816078252218	22.197700328847915
17	20.022430514023736	29.421002366925382	27.42143659184057	23.13513052721032
18	20.404956003020587	28.896832510484828	28.828909218522547	21.86930226797204
19	22.87090043051389	28.21989898303911	27.874609002041378	21.034591584405625
20	22.452297572602227	28.844089958855406	27.735849056603772	20.967763411938588
21	23.22561316609411	26.62485686133525	27.549599211738702	22.599930760831935
22	22.41842886421657	28.12671042696252	27.26229143611276	22.192569272708145
23	22.514555648310505	28.103551804527594	27.773894496347275	21.607998050814622
24	22.32911716025497	28.74205888971594	27.25483590480388	21.673988045225215
25	21.97917498868206	28.24811589571516	28.054512529626376	21.718196585976404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	401.0
1	401.0
2	401.0
3	401.0
4	401.0
5	401.0
6	1185.0
7	1969.0
8	1969.0
9	1969.0
10	2163.5
11	2358.0
12	2358.0
13	2358.0
14	3447.0
15	4536.0
16	4536.0
17	4536.0
18	8395.0
19	12254.0
20	12254.0
21	12254.0
22	17677.5
23	23101.0
24	23101.0
25	23101.0
26	33991.5
27	44882.0
28	44882.0
29	44882.0
30	54953.5
31	65025.0
32	65025.0
33	65025.0
34	70586.0
35	76147.0
36	76147.0
37	76147.0
38	79531.5
39	82916.0
40	82916.0
41	82916.0
42	83514.5
43	84113.0
44	84113.0
45	84113.0
46	90933.5
47	97754.0
48	97754.0
49	97754.0
50	90975.5
51	84197.0
52	84197.0
53	84197.0
54	73921.5
55	63646.0
56	63646.0
57	63646.0
58	53246.0
59	42846.0
60	42846.0
61	42846.0
62	35140.5
63	27435.0
64	27435.0
65	27435.0
66	22388.0
67	17341.0
68	17341.0
69	17341.0
70	13987.0
71	10633.0
72	10633.0
73	10633.0
74	8299.5
75	5966.0
76	5966.0
77	5966.0
78	4085.5
79	2205.0
80	2205.0
81	2205.0
82	1520.0
83	835.0
84	835.0
85	835.0
86	563.5
87	292.0
88	292.0
89	292.0
90	195.5
91	99.0
92	99.0
93	99.0
94	73.0
95	47.0
96	47.0
97	47.0
98	161.0
99	275.0
100	275.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.982183765834658E-4
2	1.3303639609724428E-4
3	3.991091882917329E-4
4	0.008913438538515367
5	0.031529625875046895
6	0.05960030545156544
7	0.10203891580658636
8	0.10669518966998992
9	0.11348004587094938
10	0.12598546710409034
11	0.1351649784348002
12	0.12731583106506278
13	0.12385688476653443
14	0.11055324515681
15	0.13024263177920217
16	0.11587470100069977
17	0.12119615684458955
18	0.11002109957242102
19	0.09458887762514069
20	0.08833616700857021
21	0.08700580304759775
22	0.10177284301439188
23	0.07862451009347136
24	0.09006564015783439
25	0.08700580304759775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	751674.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.791874361417705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.06847511263655	23.33437959730721
2	15.818286395762534	11.00695665788586
3	6.5136676255869705	6.798681169843676
4	3.1096057115092277	4.327560449135038
5	1.7359021869551523	3.0197645396126944
6	1.119027778163624	2.335984431888313
7	0.7549111558612424	1.8385341862129874
8	0.49150366287326086	1.3680266949490476
9	0.39143200255705535	1.2256787748602882
>10	2.4359296549233584	16.675627321560935
>50	0.31830288842956406	7.817851980817854
>100	0.22335021230189558	14.854212864017486
>500	0.018067917346280694	4.484712109051513
>1k	0.0015376950933004845	0.9120292228571251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2348	0.3123694580363296	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1938	0.25782453563645946	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1364	0.18146164427664122	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1169	0.15551954703767856	No Hit
CTCTAATACTTGTAATGCTAGAGGT	956	0.12718279466896554	No Hit
CCATTGGGATGTCCTGATCCAACAT	950	0.12638457629238206	No Hit
GTCAGGATACCGCGGCCGTTAAACT	927	0.12332473918214544	No Hit
GTTATATAATTTAAGCTCCATAGGG	923	0.12279259359775647	No Hit
GATTAAACCTTGTACCTTTTGCATA	896	0.11920061090313087	No Hit
GGTATCAACGCAGAGTACTTTTTTT	891	0.11853542892264467	No Hit
CCTATAACTTCTCTGTTAACCCAAC	886	0.11787024694215843	No Hit
ATAAATAATCCACCTATAACTTCTC	886	0.11787024694215843	No Hit
GACTATAGGCAATAATCACACTATA	860	0.11441130064363009	No Hit
ACCTATAACTTCTCTGTTAACCCAA	850	0.11308093668265765	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	841	0.11188360911778245	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	840	0.1117505727216852	No Hit
CACTATAAATAATCCACCTATAACT	838	0.11148449992949071	No Hit
CTTCTACACCATTGGGATGTCCTGA	838	0.11148449992949071	No Hit
CTATAGAACTAGTACCGCAAGGGAA	838	0.11148449992949071	No Hit
CTGTTAGTATGAGTAACAAGAATTC	800	0.10642911687779542	No Hit
ATCTATAACTTTATAGATGCAACAC	791	0.10523178931292022	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	761	0.10124069743000291	No Hit
CTGTTAACCCAACACCGGAATGCCT	757	0.10070855184561392	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	756	0.10057551544951668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.3303639609724428E-4	0.0
6	0.0	0.0	0.0	1.3303639609724428E-4	0.0
7	0.0	0.0	0.0	1.3303639609724428E-4	0.0
8	0.0	0.0	0.0	1.3303639609724428E-4	0.0
9	0.0	0.0	0.0	1.3303639609724428E-4	0.0
10	0.0	0.0	0.0	1.3303639609724428E-4	0.0
11	0.0	0.0	0.0	1.3303639609724428E-4	0.0
12	0.0	0.0	0.0	1.3303639609724428E-4	0.0
13	0.0	0.0	0.0	1.3303639609724428E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAT	45	6.7857374E-4	14.769382	1
TGCGTCA	40	0.005269964	14.252351	10
GTCCGAC	60	2.5623913E-5	14.251401	12
CGTACTC	75	9.657324E-7	13.932845	3
TACACAG	55	1.9563938E-4	13.819539	5
TAGGACC	110	1.8553692E-10	13.817697	4
GTTAAGC	90	3.630521E-8	13.72174	15
GCGTTCA	85	2.6895032E-7	13.413083	13
GGTATCA	520	0.0	12.963785	1
AACGTAA	110	2.730303E-9	12.956681	10
CTGTAGG	255	0.0	12.6594715	1
AGGACCT	130	1.9645086E-10	12.424297	5
TGTAGGA	295	0.0	12.233597	2
GTACTAG	70	1.0952455E-4	12.207347	1
GTTACAA	125	1.4060788E-9	12.159573	15
CGTCCGA	55	0.003060588	12.093709	11
CTATCCT	110	3.803507E-8	12.090485	4
CCTACAT	55	0.003066708	12.090485	3
CGACATG	55	0.003066708	12.090485	15
TACACCT	135	3.6925485E-10	11.964139	5
>>END_MODULE