FastQCFastQC Report
Thu 2 Feb 2017
SRR4062036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751674
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG18540.24664947836429088No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT11760.15645080181035928No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT9260.12319170278604821No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA9240.1229256299938537No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC9150.12172830242897853No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA9040.12026490207190885No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC9010.11986579288361711No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC8800.11707202856557498No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC8610.11454433703972733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.11401219145533836No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC8550.11374611866314385No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC8460.11254879109826868No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT8420.11201664551387967No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA8300.11042020876071276No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG8250.10975502678022653No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG8160.10855769921535134No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC8110.1078925172348651No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA7930.10549786210511472No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA7690.10230498859878086No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7590.10097462463780842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14750.026.7065411
TTAGGAC2500.025.5193823
GGACGTG3700.024.9723686
TAGGACG3950.023.9487884
GGTATCA5700.022.392871
GACGTGA2650.021.5843837
CTGTAGG3500.020.1204911
TGTAGGA4050.020.098282
GGCTAGC550.004474067620.006171
TTAACAC550.004482690219.9995163
AACACGC550.004482690219.99951632
ATATGGC7600.019.68373515
TAGGACA1705.456968E-1219.4112954
AGGGGTC1605.2750693E-1119.2495375
TGTACCG708.120738E-418.8566885
TAGTCGG951.5961328E-518.52586740
CTTTATG600.007410595718.3328882
CCCTGTA600.007410595718.3328882
AGGACGT5450.018.16475
AACGCAG22150.017.877676
Produced by FastQC (version 0.11.5)