##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062036_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 751674 Sequences flagged as poor quality 0 Sequence length 50 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.288877891213478 32.0 32.0 32.0 32.0 32.0 2 31.498601787477018 32.0 32.0 32.0 32.0 32.0 3 31.610112894685727 32.0 32.0 32.0 32.0 32.0 4 31.67902308713618 32.0 32.0 32.0 32.0 32.0 5 31.659627710949163 32.0 32.0 32.0 32.0 32.0 6 35.28818078049793 36.0 36.0 36.0 36.0 36.0 7 35.29507738727161 36.0 36.0 36.0 36.0 36.0 8 35.25915356923347 36.0 36.0 36.0 36.0 36.0 9 35.34087516662809 36.0 36.0 36.0 36.0 36.0 10 35.237324957361835 36.0 36.0 36.0 36.0 36.0 11 35.350747797582464 36.0 36.0 36.0 36.0 36.0 12 35.26899826254467 36.0 36.0 36.0 36.0 36.0 13 35.313324659360305 36.0 36.0 36.0 36.0 36.0 14 35.285822045195125 36.0 36.0 36.0 36.0 36.0 15 35.264642650936445 36.0 36.0 36.0 36.0 36.0 16 35.28632226204445 36.0 36.0 36.0 36.0 36.0 17 35.27398579703435 36.0 36.0 36.0 36.0 36.0 18 35.26526659163414 36.0 36.0 36.0 36.0 36.0 19 35.247640599515215 36.0 36.0 36.0 36.0 36.0 20 35.24432666288843 36.0 36.0 36.0 36.0 36.0 21 35.233324552931194 36.0 36.0 36.0 36.0 36.0 22 35.22020450354808 36.0 36.0 36.0 36.0 36.0 23 35.20969861934828 36.0 36.0 36.0 36.0 36.0 24 35.18267360584509 36.0 36.0 36.0 36.0 36.0 25 35.18762389014387 36.0 36.0 36.0 36.0 36.0 26 35.15046283362202 36.0 36.0 36.0 36.0 36.0 27 35.13020405122433 36.0 36.0 36.0 36.0 36.0 28 35.1158680491809 36.0 36.0 36.0 36.0 36.0 29 35.10939183741888 36.0 36.0 36.0 36.0 36.0 30 35.072776230121036 36.0 36.0 36.0 36.0 36.0 31 35.06722595167586 36.0 36.0 36.0 36.0 36.0 32 35.04834276561382 36.0 36.0 36.0 36.0 36.0 33 35.0310573466689 36.0 36.0 36.0 36.0 36.0 34 35.024614394005916 36.0 36.0 36.0 36.0 36.0 35 35.00265008501026 36.0 36.0 36.0 36.0 36.0 36 34.97571420589245 36.0 36.0 36.0 36.0 36.0 37 34.956068721280765 36.0 36.0 36.0 36.0 36.0 38 34.950364120616115 36.0 36.0 36.0 36.0 36.0 39 34.912968920037144 36.0 36.0 36.0 36.0 36.0 40 34.92454175613364 36.0 36.0 36.0 36.0 36.0 41 34.88941482610813 36.0 36.0 36.0 36.0 36.0 42 34.876028703932825 36.0 36.0 36.0 36.0 36.0 43 34.85578322517474 36.0 36.0 36.0 36.0 36.0 44 34.81530158020631 36.0 36.0 36.0 32.0 36.0 45 34.792336837512 36.0 36.0 36.0 32.0 36.0 46 34.730403073672896 36.0 36.0 36.0 32.0 36.0 47 34.708650292547034 36.0 36.0 36.0 32.0 36.0 48 34.68543943251995 36.0 36.0 36.0 32.0 36.0 49 34.674096749388696 36.0 36.0 36.0 32.0 36.0 50 34.25730702405564 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 6.0 21 19.0 22 35.0 23 109.0 24 249.0 25 568.0 26 1389.0 27 2877.0 28 5039.0 29 8313.0 30 12841.0 31 19456.0 32 29627.0 33 50556.0 34 116503.0 35 504085.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05711646995729 22.13516056355272 13.03897680075907 24.768746165730917 2 14.858743156676358 21.712244477113533 39.662606682676 23.766405683534114 3 16.24540523713167 26.99891308830592 31.06824661321243 25.687435061349976 4 10.723265671022277 18.188470001623045 41.03747103132475 30.050793296029926 5 11.518823319683799 39.76431272067412 36.04421597660688 12.672647983035198 6 28.186554277519242 41.71781916096606 18.225055010549788 11.870571550964913 7 26.789139972913787 33.656079630265246 22.409581813392506 17.145198583428453 8 25.448133100253568 35.50289088088719 20.12015847295503 18.92881754590421 9 25.589151680116647 15.351867964037602 21.773401767255486 37.28557858859027 10 14.562030880408262 27.291219331784788 34.075676423555954 24.071073364250992 11 32.65617807719836 24.729470488536254 24.891109709794407 17.72324172447098 12 21.814243940857338 27.336451706457854 30.435667589939257 20.41363676274555 13 25.722188076213893 22.15268853252873 28.42176262581917 23.70336076543821 14 20.821127243991413 22.912725463432285 27.517514241546202 28.748633051030104 15 23.79901393423213 29.76503111721305 23.697507164009927 22.738447784544896 16 23.150840932009693 28.93532499807095 25.73523346805629 22.17860060186306 17 20.008940045817734 29.376431804213 27.338580288795413 23.27604786117386 18 20.570486673744202 28.64167178856792 28.924640203066755 21.863201334621127 19 23.01290186969351 28.047797316389815 28.047930352785915 20.891370461130755 20 22.647009130324307 28.672916741499936 27.798331983008524 20.881742145167234 21 23.40402887421941 26.510827832278355 27.614231701508903 22.470911591993335 22 22.44124447566365 27.949882528862247 27.495962345378448 22.112910650095653 23 22.6705992225353 27.78066023302655 27.829351553998144 21.719388990440006 24 22.47987143260056 28.788640729683852 27.103721323775922 21.627766513939665 25 22.11911860375084 28.032343937706788 28.23362880834833 21.614908650194035 26 21.201828991673217 27.74711276609048 29.290873267692913 21.760184974543385 27 21.266484938224302 29.02134045346967 27.467203071556572 22.244971536749457 28 21.497903318504015 27.528257306456076 28.573006023968155 22.400833351071757 29 21.956073750547116 28.010467373272014 27.530601900570595 22.502856975610275 30 22.328726665105687 27.625109091296114 28.025820047255156 22.020344196343046 31 22.628783071317105 26.850709951561253 28.059483470826997 22.46102350629464 32 20.81759608975783 27.953867869681652 28.18096311265494 23.047572927905573 33 21.93496631509488 28.123303781436583 28.029379835885866 21.91235006758267 34 21.690207550909907 27.502058746446252 29.327458422634372 21.480275280009472 35 22.29549590778957 28.13168509669111 27.439095775817112 22.133723219702212 36 21.659817500429043 27.296710138584196 28.085483980401055 22.957988380585707 37 23.11907565205244 26.911190490444547 26.59123412690494 23.378499730598072 38 22.083509606558163 27.932587797369607 27.935913707272036 22.047988888800198 39 21.83806968226706 28.29370374460035 26.874595067775843 22.993631505356745 40 22.170175289221895 28.03217360763737 28.238779680498943 21.55887142264179 41 22.29345629525762 27.70980311812779 28.23862631025092 21.758114276363667 42 21.982776397837803 28.502626911978158 27.262676563208863 22.251920126975172 43 21.18685558438103 28.42639526375308 27.819330805561098 22.567418346304795 44 21.628075907871768 27.947523308782834 27.76392673166163 22.660474051683764 45 21.878762480626342 28.213366326754606 28.093366060678626 21.814505131940425 46 23.1694564844305 27.44717705564469 26.75497763622479 22.628388823700014 47 22.43771477377495 28.488165574200142 26.739642632403083 22.334477019621826 48 21.92247005952175 28.76002117984509 26.483719989676157 22.833788770957003 49 22.107895962216457 27.780216856249584 27.94545333599415 22.166433845539814 50 21.209686217016603 28.57199873348003 27.30494661192604 22.91336843757733 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 160.0 1 99.5 2 39.0 3 124.0 4 209.0 5 201.5 6 194.0 7 146.0 8 98.0 9 155.5 10 213.0 11 356.5 12 500.0 13 906.5 14 1313.0 15 1754.0 16 2195.0 17 2691.5 18 3188.0 19 3242.5 20 3297.0 21 4196.0 22 5095.0 23 5522.0 24 5949.0 25 8772.0 26 11595.0 27 16218.5 28 20842.0 29 23577.0 30 26312.0 31 26672.5 32 27033.0 33 30734.0 34 34435.0 35 38664.0 36 42893.0 37 48877.0 38 54861.0 39 55814.5 40 56768.0 41 58689.0 42 60610.0 43 54800.0 44 48990.0 45 47553.0 46 46116.0 47 45646.0 48 45176.0 49 42591.5 50 40007.0 51 40608.0 52 41209.0 53 38496.0 54 35783.0 55 33522.0 56 31261.0 57 30147.0 58 29033.0 59 25345.0 60 21657.0 61 19318.5 62 16980.0 63 14344.0 64 11708.0 65 9900.0 66 8092.0 67 6747.5 68 5403.0 69 4631.5 70 3860.0 71 3140.5 72 2421.0 73 2421.0 74 2421.0 75 2082.0 76 1743.0 77 1366.5 78 990.0 79 752.5 80 515.0 81 418.0 82 321.0 83 211.0 84 101.0 85 72.5 86 44.0 87 30.0 88 16.0 89 11.5 90 7.0 91 7.5 92 8.0 93 5.5 94 3.0 95 3.5 96 4.0 97 4.5 98 5.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030199261914074453 2 0.0038580554868200847 3 3.991091882917329E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0010642911687779543 17 0.0 18 0.0 19 0.0 20 3.991091882917329E-4 21 0.0 22 0.0 23 0.0 24 7.982183765834658E-4 25 3.991091882917329E-4 26 3.991091882917329E-4 27 3.991091882917329E-4 28 0.0013303639609724429 29 6.651819804862214E-4 30 0.0013303639609724429 31 3.991091882917329E-4 32 9.3125477268071E-4 33 2.6607279219448857E-4 34 3.991091882917329E-4 35 2.6607279219448857E-4 36 1.3303639609724428E-4 37 0.0011973275648751985 38 0.0 39 6.651819804862214E-4 40 2.6607279219448857E-4 41 6.651819804862214E-4 42 0.004124128279014572 43 0.003192873506333863 44 0.003458946298528351 45 0.0011973275648751985 46 0.0015964367531669315 47 0.001463400357069687 48 0.0021285823375559085 49 0.003192873506333863 50 0.0010642911687779543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 751674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.151863489355904 #Duplication Level Percentage of deduplicated Percentage of total 1 66.88543066073203 24.849183893342968 2 16.17811910527638 12.020945450275375 3 6.504191405659457 7.249284936351062 4 3.2687551044434215 4.857613736816692 5 1.822202400559823 3.3849107417787585 6 1.0879751265969912 2.425218202988768 7 0.725349697050483 1.8863665048805645 8 0.49219839390878417 1.4628870032143502 9 0.35347153835024653 1.181891370614489 >10 2.2027053497635 16.047804471311608 >50 0.2753399286285702 7.116162068158725 >100 0.1884660399664608 13.105089871719736 >500 0.015077283197316597 4.008534135112297 >1k 7.179658665388856E-4 0.40410761343459745 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 1854 0.24664947836429088 No Hit GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT 1176 0.15645080181035928 No Hit CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT 926 0.12319170278604821 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 924 0.1229256299938537 No Hit GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC 915 0.12172830242897853 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 904 0.12026490207190885 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 901 0.11986579288361711 No Hit GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC 880 0.11707202856557498 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 861 0.11454433703972733 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 857 0.11401219145533836 No Hit GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC 855 0.11374611866314385 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 846 0.11254879109826868 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 842 0.11201664551387967 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 830 0.11042020876071276 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 825 0.10975502678022653 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 816 0.10855769921535134 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 811 0.1078925172348651 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 793 0.10549786210511472 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 769 0.10230498859878086 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 759 0.10097462463780842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 2.6607279219448857E-4 0.0 18 0.0 0.0 0.0 2.6607279219448857E-4 0.0 19 0.0 0.0 0.0 2.6607279219448857E-4 0.0 20 0.0 0.0 0.0 2.6607279219448857E-4 0.0 21 0.0 0.0 0.0 2.6607279219448857E-4 0.0 22 0.0 0.0 0.0 2.6607279219448857E-4 0.0 23 0.0 0.0 0.0 2.6607279219448857E-4 0.0 24 0.0 0.0 0.0 3.991091882917329E-4 0.0 25 0.0 0.0 0.0 3.991091882917329E-4 0.0 26 0.0 0.0 0.0 5.321455843889771E-4 0.0 27 0.0 0.0 0.0 6.651819804862214E-4 0.0 28 0.0 0.0 0.0 0.0017294731492641757 0.0 29 0.0 0.0 0.0 0.002261618733653153 0.0 30 0.0 0.0 0.0 0.003991091882917328 0.0 31 0.0 0.0 0.0 0.00838129295412639 0.0 32 0.0 0.0 0.0 0.011707202856557497 0.0 33 0.0 0.0 0.0 0.015565258343377582 0.0 34 0.0 0.0 0.0 0.022084041752142552 0.0 35 0.0 0.0 0.0 0.028602825160907522 0.0 36 0.0 0.0 0.0 0.03618589973845045 0.0 37 0.0 0.0 0.0 0.04909043015988314 0.0 38 0.0 0.0 0.0 0.06824767119788631 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1475 0.0 26.706541 1 TTAGGAC 250 0.0 25.519382 3 GGACGTG 370 0.0 24.972368 6 TAGGACG 395 0.0 23.948788 4 GGTATCA 570 0.0 22.39287 1 GACGTGA 265 0.0 21.584383 7 CTGTAGG 350 0.0 20.120491 1 TGTAGGA 405 0.0 20.09828 2 GGCTAGC 55 0.0044740676 20.00617 1 TTAACAC 55 0.0044826902 19.999516 3 AACACGC 55 0.0044826902 19.999516 32 ATATGGC 760 0.0 19.683735 15 TAGGACA 170 5.456968E-12 19.411295 4 AGGGGTC 160 5.2750693E-11 19.249537 5 TGTACCG 70 8.120738E-4 18.856688 5 TAGTCGG 95 1.5961328E-5 18.525867 40 CTTTATG 60 0.0074105957 18.332888 2 CCCTGTA 60 0.0074105957 18.332888 2 AGGACGT 545 0.0 18.1647 5 AACGCAG 2215 0.0 17.87767 6 >>END_MODULE