FastQCFastQC Report
Thu 2 Feb 2017
SRR4062035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028169
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37860.18667083462965858No Hit
TATCAACGCAGAGTACTTTTTTTTT20930.10319652849442033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG250.006034688618.99891314
GTCTACG551.967736E-413.8122761
GTATCAA19900.013.1702491
AACCGCG604.0864336E-412.6690697
CCTTACG550.003077132412.0866362
GGTATCA7500.011.9015791
TCGCGTA1209.984433E-911.877849
CGCGTAA1251.8275387E-811.402163510
CGCATCG1251.832268E-811.40019213
GCGTAAC1354.7148205E-911.26167411
GACCGCG2700.011.2613957
GTATAGG2150.011.0417921
TATACCG951.3600209E-511.0004585
GTGTTAA1658.185452E-1110.9347191
CTCTTAT1605.493348E-1010.6829331
TAGACTG2250.010.5559945
ACGGTAT1201.5194146E-610.2941279
TATAGGA2400.010.288032
GTTCTAG1201.5324658E-610.2872681
GGACCGC3800.010.2514396
Produced by FastQC (version 0.11.5)