##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062035_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2028169
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.282924647798087	32.0	32.0	32.0	32.0	32.0
2	30.86915094353577	32.0	32.0	32.0	32.0	32.0
3	30.895612742330645	32.0	32.0	32.0	32.0	32.0
4	30.900951054867715	32.0	32.0	32.0	32.0	32.0
5	30.832088450222837	32.0	32.0	32.0	32.0	32.0
6	34.46392337127725	36.0	36.0	36.0	32.0	36.0
7	34.418756030685806	36.0	36.0	36.0	32.0	36.0
8	34.39112470410503	36.0	36.0	36.0	32.0	36.0
9	34.50895117714549	36.0	36.0	36.0	32.0	36.0
10	34.240585473892956	36.0	36.0	36.0	32.0	36.0
11	34.47460196857362	36.0	36.0	36.0	32.0	36.0
12	34.34739264824578	36.0	36.0	36.0	32.0	36.0
13	34.39173214855369	36.0	36.0	36.0	32.0	36.0
14	34.32536637725949	36.0	36.0	36.0	32.0	36.0
15	34.28025721722401	36.0	36.0	36.0	32.0	36.0
16	34.2842790714186	36.0	36.0	36.0	32.0	36.0
17	34.22790901547159	36.0	36.0	36.0	32.0	36.0
18	34.236431973864114	36.0	36.0	36.0	32.0	36.0
19	34.23831051554382	36.0	36.0	36.0	32.0	36.0
20	34.21346643203796	36.0	36.0	36.0	32.0	36.0
21	34.20483845281138	36.0	36.0	36.0	32.0	36.0
22	34.18240442487781	36.0	36.0	36.0	32.0	36.0
23	34.14892003575639	36.0	36.0	36.0	32.0	36.0
24	34.12117333417481	36.0	36.0	36.0	32.0	36.0
25	33.779667769303245	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	29.0
5	150.0
6	420.0
7	81.0
8	359.0
9	232.0
10	143.0
11	44.0
12	73.0
13	102.0
14	288.0
15	442.0
16	688.0
17	937.0
18	1461.0
19	1816.0
20	2636.0
21	3772.0
22	5432.0
23	7709.0
24	10971.0
25	15465.0
26	21323.0
27	27864.0
28	37630.0
29	49610.0
30	64983.0
31	89159.0
32	127792.0
33	183425.0
34	415372.0
35	957761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51072310447385	16.707837313326866	11.906156564819854	25.875283017379434
2	16.658112135846935	18.56855827804937	37.73548996260166	27.03783962350203
3	19.73249539482248	21.834606084751044	27.838593951538147	30.594304568888326
4	13.278341812343408	15.019657792183136	34.40476067198929	37.29723972348417
5	15.295835793267843	35.74014805476517	33.36904245785312	15.594973694113865
6	35.98456779464511	34.06614929967981	15.992362834434342	13.956920071240745
7	31.51164380485219	29.660125576686823	19.3127092471766	19.51552137128439
8	28.37870720857011	33.64218203284372	18.16740119416452	19.811709564421655
9	26.892282388389177	14.891447176817055	18.009965278497646	40.206305156296125
10	16.093259772157836	27.055207909060613	31.232149689586652	25.6193826291949
11	37.1215970083742	21.516974286889372	21.70523460454718	19.656194100189246
12	24.64875651561724	23.131515000113552	28.445316210470605	23.774412273798603
13	29.62269618667769	19.6667767570148	24.68358898484357	26.02693807146394
14	23.42059292027972	19.124811007216156	24.924686714998142	32.52990935750598
15	24.939361519879498	27.118742807434504	21.96724904950756	25.974646623178437
16	25.59284191425284	26.28866819067891	23.228977491777293	24.889512403290958
17	24.234772585346832	26.185105926084994	24.72141797547027	24.85870351309791
18	24.706681974651005	25.780988549614552	25.26745371819159	24.244875757542854
19	25.54982028404153	25.165416164713882	25.020172703013888	24.2645908482307
20	25.76221535825516	24.940299442893497	24.794817118157013	24.502668080694328
21	25.873607195431102	24.562758432824367	24.818039110796157	24.74559526094837
22	25.757023128362633	24.484447421114275	24.843753624526006	24.914775825997086
23	24.79371304673074	24.681650453530292	25.207619269436766	25.3170172303022
24	24.817667016223552	24.83493923878848	25.1097649744001	25.23762877058787
25	24.985701716336855	24.724162791696088	25.07023307065943	25.219902421307626
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	87.0
2	292.5
3	498.0
4	498.0
5	498.0
6	1063.5
7	1629.0
8	1629.0
9	1629.0
10	2150.5
11	2672.0
12	2672.0
13	2672.0
14	3813.5
15	4955.0
16	4955.0
17	4955.0
18	8446.0
19	11937.0
20	11937.0
21	11937.0
22	18395.5
23	24854.0
24	24854.0
25	24854.0
26	35867.0
27	46880.0
28	46880.0
29	46880.0
30	60976.0
31	75072.0
32	75072.0
33	75072.0
34	94017.5
35	112963.0
36	112963.0
37	112963.0
38	137364.0
39	161765.0
40	161765.0
41	161765.0
42	187427.5
43	213090.0
44	213090.0
45	213090.0
46	237782.0
47	262474.0
48	262474.0
49	262474.0
50	274433.0
51	286392.0
52	286392.0
53	286392.0
54	274090.0
55	261788.0
56	261788.0
57	261788.0
58	239291.5
59	216795.0
60	216795.0
61	216795.0
62	187494.5
63	158194.0
64	158194.0
65	158194.0
66	127706.5
67	97219.0
68	97219.0
69	97219.0
70	73109.0
71	48999.0
72	48999.0
73	48999.0
74	35673.5
75	22348.0
76	22348.0
77	22348.0
78	16348.0
79	10348.0
80	10348.0
81	10348.0
82	7205.0
83	4062.0
84	4062.0
85	4062.0
86	2832.5
87	1603.0
88	1603.0
89	1603.0
90	1046.0
91	489.0
92	489.0
93	489.0
94	335.5
95	182.0
96	182.0
97	182.0
98	528.0
99	874.0
100	874.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012326388974488811
2	1.97222223591821E-4
3	8.381944502652393E-4
4	0.010847222297550155
5	0.033330555787017754
6	0.06108958375756656
7	0.10630277851599151
8	0.1060069451806038
9	0.11512847302172549
10	0.13036388979419367
11	0.1371680565081115
12	0.13070902868547937
13	0.12622222309876543
14	0.11330416745350116
15	0.13016666757060186
16	0.11715000081354165
17	0.12148888973256174
18	0.10901458409037906
19	0.09446944510048226
20	0.08904583395170718
21	0.0873694450511767
22	0.10068194514362462
23	0.08002291722238138
24	0.08845416728093171
25	0.08391805613831983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2028169.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.63102999286025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.70723191812178	37.74025473787653
2	15.013417718065758	15.803432764297169
3	5.562546818389704	8.782877053060735
4	2.597096855165546	5.467515299143234
5	1.4188559537332268	3.7337925128240896
6	0.8692131834861554	2.744855107814963
7	0.5750805159661734	2.118695591888765
8	0.4090764040788746	1.722408999395733
9	0.28864312132763803	1.3672426298244944
>10	1.4448746665709133	13.328377805677743
>50	0.07747080729102462	2.773214915874752
>100	0.03414182157778556	3.310911801894371
>500	0.0019741816293086284	0.696893689845306
>1k	3.760345960587858E-4	0.40952709058215053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3786	0.18667083462965858	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2093	0.10319652849442033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.930555589795525E-5	1.97222223591821E-4
2	0.0	0.0	0.0	4.930555589795525E-5	1.97222223591821E-4
3	0.0	0.0	0.0	4.930555589795525E-5	1.97222223591821E-4
4	0.0	0.0	0.0	4.930555589795525E-5	1.97222223591821E-4
5	0.0	0.0	0.0	4.930555589795525E-5	1.97222223591821E-4
6	0.0	0.0	0.0	4.930555589795525E-5	3.94444447183642E-4
7	0.0	0.0	0.0	4.930555589795525E-5	3.94444447183642E-4
8	0.0	0.0	0.0	4.930555589795525E-5	3.94444447183642E-4
9	0.0	0.0	0.0	4.930555589795525E-5	3.94444447183642E-4
10	0.0	0.0	0.0	9.86111117959105E-5	4.930555589795525E-4
11	0.0	0.0	0.0	9.86111117959105E-5	4.930555589795525E-4
12	0.0	0.0	0.0	9.86111117959105E-5	0.001380555565142747
13	0.0	0.0	0.0	9.86111117959105E-5	0.0016270833446325231
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGG	25	0.0060346886	18.998913	14
GTCTACG	55	1.967736E-4	13.812276	1
GTATCAA	1990	0.0	13.170249	1
AACCGCG	60	4.0864336E-4	12.669069	7
CCTTACG	55	0.0030771324	12.086636	2
GGTATCA	750	0.0	11.901579	1
TCGCGTA	120	9.984433E-9	11.87784	9
CGCGTAA	125	1.8275387E-8	11.4021635	10
CGCATCG	125	1.832268E-8	11.400192	13
GCGTAAC	135	4.7148205E-9	11.261674	11
GACCGCG	270	0.0	11.261395	7
GTATAGG	215	0.0	11.041792	1
TATACCG	95	1.3600209E-5	11.000458	5
GTGTTAA	165	8.185452E-11	10.934719	1
CTCTTAT	160	5.493348E-10	10.682933	1
TAGACTG	225	0.0	10.555994	5
ACGGTAT	120	1.5194146E-6	10.294127	9
TATAGGA	240	0.0	10.28803	2
GTTCTAG	120	1.5324658E-6	10.287268	1
GGACCGC	380	0.0	10.251439	6
>>END_MODULE