FastQCFastQC Report
Thu 2 Feb 2017
SRR4062035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028169
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21700.027.8859041
GGTATCA8650.026.9650671
TCAACGC30850.019.3249684
ATCAACG31750.018.7771743
CAACGCA31700.018.7373945
GTATTAG2000.018.7038351
TATCAAC32000.018.6309382
AACGCAG32550.018.4508486
CCGATCG1102.7994301E-617.9992549
CGCGTAA2250.017.5992710
GCGTAAC2300.017.21667711
CGGATCG1501.8397259E-716.13306226
TCGCGTA2600.016.0762589
CCTAATA2051.8117134E-915.0241382
GTATAAG4400.015.00307751
ACGCAGA39700.014.9615967
CGCAATA1951.5168553E-814.66714336
ATCGATT1655.423153E-714.6664229
CGCAGAG40800.014.5582218
GCATATA3950.014.4839841
Produced by FastQC (version 0.11.5)