##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2028169
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293131390924522	32.0	32.0	32.0	32.0	32.0
2	31.444319975307778	32.0	32.0	32.0	32.0	32.0
3	31.560728420560615	32.0	32.0	32.0	32.0	32.0
4	31.64439304614162	32.0	32.0	32.0	32.0	32.0
5	31.61305788620179	32.0	32.0	32.0	32.0	32.0
6	35.258925661520315	36.0	36.0	36.0	36.0	36.0
7	35.26585358517954	36.0	36.0	36.0	36.0	36.0
8	35.21208587647282	36.0	36.0	36.0	36.0	36.0
9	35.30346830071853	36.0	36.0	36.0	36.0	36.0
10	35.18328995266174	36.0	36.0	36.0	36.0	36.0
11	35.30628907157145	36.0	36.0	36.0	36.0	36.0
12	35.23416687662616	36.0	36.0	36.0	36.0	36.0
13	35.26585703656845	36.0	36.0	36.0	36.0	36.0
14	35.23425858496013	36.0	36.0	36.0	36.0	36.0
15	35.20610412643128	36.0	36.0	36.0	36.0	36.0
16	35.2215949459833	36.0	36.0	36.0	36.0	36.0
17	35.20628951532146	36.0	36.0	36.0	36.0	36.0
18	35.19803083470855	36.0	36.0	36.0	36.0	36.0
19	35.18933284159259	36.0	36.0	36.0	36.0	36.0
20	35.19737802914846	36.0	36.0	36.0	36.0	36.0
21	35.188860001311525	36.0	36.0	36.0	36.0	36.0
22	35.16985270951287	36.0	36.0	36.0	36.0	36.0
23	35.14133289681481	36.0	36.0	36.0	36.0	36.0
24	35.128424702280725	36.0	36.0	36.0	36.0	36.0
25	35.11522807024464	36.0	36.0	36.0	36.0	36.0
26	35.07451450051746	36.0	36.0	36.0	36.0	36.0
27	35.065583785177665	36.0	36.0	36.0	36.0	36.0
28	35.046972416992865	36.0	36.0	36.0	36.0	36.0
29	35.03085097938091	36.0	36.0	36.0	36.0	36.0
30	35.0118851042492	36.0	36.0	36.0	36.0	36.0
31	35.00802447922239	36.0	36.0	36.0	36.0	36.0
32	34.98140736792644	36.0	36.0	36.0	36.0	36.0
33	34.97071447201885	36.0	36.0	36.0	36.0	36.0
34	34.96006989555604	36.0	36.0	36.0	36.0	36.0
35	34.95083693715859	36.0	36.0	36.0	36.0	36.0
36	34.92581584670705	36.0	36.0	36.0	36.0	36.0
37	34.913795152179134	36.0	36.0	36.0	36.0	36.0
38	34.90838386741933	36.0	36.0	36.0	36.0	36.0
39	34.88382131863765	36.0	36.0	36.0	32.0	36.0
40	34.8779598741525	36.0	36.0	36.0	32.0	36.0
41	34.84964566562254	36.0	36.0	36.0	32.0	36.0
42	34.82893782520096	36.0	36.0	36.0	32.0	36.0
43	34.82080438070003	36.0	36.0	36.0	32.0	36.0
44	34.7863526165719	36.0	36.0	36.0	32.0	36.0
45	34.7745996512125	36.0	36.0	36.0	32.0	36.0
46	34.75209659550067	36.0	36.0	36.0	32.0	36.0
47	34.72508947725757	36.0	36.0	36.0	32.0	36.0
48	34.70957203270536	36.0	36.0	36.0	32.0	36.0
49	34.68852842144811	36.0	36.0	36.0	32.0	36.0
50	34.280571786670635	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	10.0
21	38.0
22	122.0
23	332.0
24	849.0
25	2074.0
26	4380.0
27	8517.0
28	15025.0
29	23858.0
30	36528.0
31	54278.0
32	82831.0
33	141966.0
34	325246.0
35	1332111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31235922139041	17.091454124976636	12.290764824570186	26.30542182906277
2	16.122347042775214	19.004204496202544	37.50650249720547	27.36694596381678
3	19.134467562255875	22.266416588024033	28.0198524575453	30.579263392174788
4	12.923824395304337	15.384319551279996	34.51684746192255	37.17500859149311
5	15.011372326467864	36.126871084214386	33.174700924824315	15.687055664493442
6	36.173944256069646	34.13505889800988	15.883849801323066	13.807147044597412
7	31.58706202491015	29.665624511566836	19.26718138379987	19.480132079723138
8	28.426132141848136	33.7369814842846	18.062104292097946	19.77478208176932
9	26.987839770748888	14.69749315762148	17.9523501246691	40.36231694696053
10	16.0463610738169	27.14150264746443	31.22499281370814	25.587143465010527
11	37.47266623244907	21.29901403679871	21.65268278925474	19.57563694149748
12	24.712191143834662	23.10073766042179	28.436683530810303	23.75038766493325
13	29.7364272898363	19.506017496569566	24.802617533351512	25.954937680242622
14	23.542367524599776	18.874166797737267	24.920457811947625	32.66300786571533
15	25.082229340848816	27.112533521614818	21.877318901925825	25.92791823561054
16	25.7295945510853	26.21580191179436	23.175440438981987	24.87916309813835
17	24.39505780829901	26.168233515057178	24.605050170868402	24.831658505775405
18	24.929308089521225	25.66366576322364	25.215724346170703	24.19130180108443
19	25.726948789770475	25.07389670190206	25.001713368067453	24.197441140260008
20	25.891864867076958	24.806302837144496	24.80161880240572	24.50021349337283
21	26.095803653443078	24.474045308847536	24.80187795001304	24.62827308769634
22	25.90597726323595	24.276823085255717	24.901277950703317	24.91592170080501
23	25.04448598167705	24.56691189630834	25.156902759979825	25.23169936203478
24	25.064221214843506	24.687473189799945	25.09612227127633	25.15218332408022
25	25.301319469884813	24.51918312285551	25.0884174496823	25.09107995757737
26	25.01015698958662	24.981017276743454	25.134308930261913	24.874516803408014
27	25.11217063742506	24.988462448721993	24.984419375197223	24.914947538655728
28	25.31978855471795	24.70109267766428	25.051759323950996	24.927359443666774
29	25.023075148065534	24.966077560108786	25.102309684264917	24.90853760756076
30	25.08974923464772	24.98181841490106	25.02575005509945	24.902682295351777
31	25.303723572104765	24.685626380561693	24.779997633322814	25.23065241401073
32	25.2034360276034	24.977516522397686	24.705742556292513	25.1133048937064
33	25.022853204033996	24.902843066776718	24.89589095940068	25.178412769788604
34	25.41031255593154	24.728360687503727	25.07355228831807	24.787774468246663
35	25.32931261968953	24.787788364254567	25.21137343922878	24.67152557682712
36	25.014052118073295	24.964647829268245	25.048418175256042	24.97288187740242
37	25.476989708776927	24.632864857189062	24.93249959568786	24.957645838346146
38	25.122770894718784	24.83906658619996	24.942460387896862	25.0957021311844
39	25.444344517877322	24.811010592873526	24.82752805997172	24.917116829277433
40	25.588092887860263	24.718144372797887	24.762667355630654	24.931095383711195
41	24.947020579386297	24.984148084013675	25.06856015296729	25.000271183632737
42	25.445154482608995	24.89812924666923	24.84980720491504	24.80690906580673
43	25.320107609804012	24.495036655575653	25.063558026653936	25.1212977079664
44	24.9598635162321	24.8528164569445	24.983481913928447	25.203838112894957
45	25.088172660139453	25.005731900875833	24.912098526568656	24.993996912416062
46	25.22244717033788	24.707044250789647	24.948647073499522	25.12186150537295
47	25.232367508240287	24.896987148354775	25.043574300654047	24.82707104275089
48	25.37121280106031	25.163430275501682	24.556502153265992	24.908854770172013
49	25.162838904618066	25.047803881310287	24.66256228803823	25.12679492603342
50	25.092548216782735	25.283265586263614	24.677535781694683	24.94665041525897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	24.0
2	33.0
3	96.0
4	159.0
5	181.5
6	204.0
7	177.5
8	151.0
9	224.5
10	298.0
11	494.0
12	690.0
13	1213.0
14	1736.0
15	2542.0
16	3348.0
17	3187.5
18	3027.0
19	3060.0
20	3093.0
21	3763.0
22	4433.0
23	5589.0
24	6745.0
25	8749.0
26	10753.0
27	13387.0
28	16021.0
29	18936.5
30	21852.0
31	25717.5
32	29583.0
33	34494.5
34	39406.0
35	45690.5
36	51975.0
37	59431.0
38	66887.0
39	74615.0
40	82343.0
41	90587.0
42	98831.0
43	105928.5
44	113026.0
45	122138.5
46	131251.0
47	138916.0
48	146581.0
49	154140.5
50	161700.0
51	163331.0
52	164962.0
53	165013.0
54	165064.0
55	162041.5
56	159019.0
57	151687.5
58	144356.0
59	132444.0
60	120532.0
61	106138.5
62	91745.0
63	78548.5
64	65352.0
65	54568.5
66	43785.0
67	36695.0
68	29605.0
69	24422.5
70	19240.0
71	14990.5
72	10741.0
73	9181.5
74	7622.0
75	6126.5
76	4631.0
77	3837.5
78	3044.0
79	2558.0
80	2072.0
81	1560.5
82	1049.0
83	842.5
84	636.0
85	473.0
86	310.0
87	217.5
88	125.0
89	87.5
90	50.0
91	40.5
92	31.0
93	24.0
94	17.0
95	17.0
96	17.0
97	17.0
98	17.0
99	11.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02987916687416088
2	0.005029166701591436
3	3.4513889128568674E-4
4	0.0
5	0.0
6	9.86111117959105E-5
7	0.0
8	0.0
9	0.0
10	9.86111117959105E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010847222297550156
17	0.0
18	9.86111117959105E-5
19	0.0
20	1.4791666769386574E-4
21	0.0
22	0.0
23	9.86111117959105E-5
24	0.0011340277856529708
25	2.958333353877315E-4
26	4.437500030815973E-4
27	4.437500030815973E-4
28	0.001577777788734568
29	6.409722266734183E-4
30	8.875000061631946E-4
31	4.437500030815973E-4
32	6.409722266734183E-4
33	3.4513889128568674E-4
34	9.86111117959105E-4
35	2.4652777948977624E-4
36	2.4652777948977624E-4
37	0.0016270833446325231
38	4.930555589795525E-5
39	6.409722266734183E-4
40	1.4791666769386574E-4
41	0.0011340277856529708
42	0.005374305592877122
43	0.00497986114569348
44	0.004388194474918017
45	0.0022680555713059415
46	0.0023173611272038967
47	0.001183333341550926
48	0.004289583363122107
49	0.004486805586713927
50	0.0018243055682243441
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2028169.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.137417605226744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69327585601816	37.5645812104273
2	15.743658508617653	16.731547136129947
3	5.827755359778006	9.29015610760867
4	2.653573520523004	5.640161772248103
5	1.434982648440759	3.812563612322544
6	0.8420986201918341	2.6848167627551227
7	0.569188035018476	2.117162761887263
8	0.4079036072195622	1.7339955455603406
9	0.29849684647696967	1.4275216426580992
>10	1.431378748690241	13.273001162273653
>50	0.06733763272214606	2.4120910921884087
>100	0.028577270120043435	2.6722748831337477
>500	0.0015866781637935295	0.5285369252551504
>1k	1.8666801926982458E-4	0.1115893855516633
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4652777948977624E-4
2	0.0	0.0	0.0	0.0	2.4652777948977624E-4
3	0.0	0.0	0.0	0.0	2.4652777948977624E-4
4	0.0	0.0	0.0	0.0	2.4652777948977624E-4
5	0.0	0.0	0.0	0.0	2.4652777948977624E-4
6	0.0	0.0	0.0	0.0	3.94444447183642E-4
7	0.0	0.0	0.0	0.0	3.94444447183642E-4
8	0.0	0.0	0.0	0.0	3.94444447183642E-4
9	0.0	0.0	0.0	0.0	3.94444447183642E-4
10	0.0	0.0	0.0	4.930555589795525E-5	4.4375000308159723E-4
11	0.0	0.0	0.0	4.930555589795525E-5	4.4375000308159723E-4
12	0.0	0.0	0.0	4.930555589795525E-5	0.0012326388974488811
13	0.0	0.0	0.0	4.930555589795525E-5	0.0014791666769386575
14	0.0	0.0	0.0	4.930555589795525E-5	0.0014791666769386575
15	0.0	0.0	0.0	4.930555589795525E-5	0.0017256944564284337
16	0.0	0.0	0.0	4.930555589795525E-5	0.001775000012326389
17	0.0	0.0	0.0	4.930555589795525E-5	0.0018243055682243441
18	0.0	0.0	0.0	9.86111117959105E-5	0.00197222223591821
19	0.0	0.0	0.0	2.4652777948977624E-4	0.002021527791816165
20	0.0	0.0	0.0	2.958333353877315E-4	0.0022680555713059415
21	0.0	0.0	0.0	4.930555589795525E-4	0.002366666683101852
22	0.0	0.0	0.0	6.409722266734183E-4	0.002514583350795718
23	0.0	0.0	0.0	8.381944502652393E-4	0.002514583350795718
24	0.0	0.0	0.0	0.0010354166738570602	0.002514583350795718
25	0.0	0.0	0.0	0.0015284722328366127	0.002514583350795718
26	0.0	0.0	0.0	0.0019229166800202547	0.0027118055743875387
27	0.0	0.0	0.0	0.002514583350795718	0.002810416686183449
28	0.0	0.0	0.0	0.002958333353877315	0.0028597222420814043
29	0.0	0.0	0.0	0.0039937500277343755	0.0028597222420814043
30	0.0	0.0	0.0	0.005127777813387346	0.0028597222420814043
31	0.0	0.0	0.0	0.0077409722759789745	0.002958333353877315
32	0.0	0.0	0.0	0.010551388962162423	0.0030076389097752703
33	0.0	0.0	0.0	0.013756250095529514	0.0030569444656732255
34	0.0	0.0	0.0	0.018193750126345488	0.003352777801060957
35	0.0	0.0	0.0	0.02233541682177373	0.003352777801060957
36	0.0	0.0	0.0	0.027364583523365165	0.003352777801060957
37	0.0	0.0	0.0	0.03535208357883391	0.003352777801060957
38	0.0	0.0	0.0	0.04703750032664931	0.003352777801060957
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2170	0.0	27.885904	1
GGTATCA	865	0.0	26.965067	1
TCAACGC	3085	0.0	19.324968	4
ATCAACG	3175	0.0	18.777174	3
CAACGCA	3170	0.0	18.737394	5
GTATTAG	200	0.0	18.703835	1
TATCAAC	3200	0.0	18.630938	2
AACGCAG	3255	0.0	18.450848	6
CCGATCG	110	2.7994301E-6	17.999254	9
CGCGTAA	225	0.0	17.59927	10
GCGTAAC	230	0.0	17.216677	11
CGGATCG	150	1.8397259E-7	16.133062	26
TCGCGTA	260	0.0	16.076258	9
CCTAATA	205	1.8117134E-9	15.024138	2
GTATAAG	440	0.0	15.0030775	1
ACGCAGA	3970	0.0	14.961596	7
CGCAATA	195	1.5168553E-8	14.667143	36
ATCGATT	165	5.423153E-7	14.66642	29
CGCAGAG	4080	0.0	14.558221	8
GCATATA	395	0.0	14.483984	1
>>END_MODULE