FastQCFastQC Report
Thu 2 Feb 2017
SRR4062034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962430
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19400.20157310142036303No Hit
TATCAACGCAGAGTACTTTTTTTTT12840.13341230011533306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT250.006041032718.9942282
AGAACCG456.755926E-414.7786675
CCCGTTT400.005267779314.25382312
AAGTGCG500.001496967313.3021857
GGTATCA3750.013.1686471
CCGACCT550.003064404712.0922669
TCTTATA550.003073969412.0872362
GTATCAA10100.011.9412951
TAAGCCT658.0179836E-411.6924044
GTATTAT658.0425094E-411.68814851
CTTAGAC1001.9315066E-611.3977223
GAATAAG752.079107E-411.3959451
CTTATAC600.005881636411.081123
CCAACGA600.00588391411.08054319
GTTTAGA600.005888470411.0793911
CCGCTCC951.356899E-511.0012359
CGCCGGT700.001488464510.8589267
CCGTCAA700.001492992910.85497418
AGGACTG2250.010.55619055
TAAACAA1002.3941693E-510.4506295
Produced by FastQC (version 0.11.5)