##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062034_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	962430
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28145735274254	32.0	32.0	32.0	32.0	32.0
2	30.858870775017404	32.0	32.0	32.0	32.0	32.0
3	30.88025622642686	32.0	32.0	32.0	32.0	32.0
4	30.880325841879408	32.0	32.0	32.0	32.0	32.0
5	30.82287854701121	32.0	32.0	32.0	32.0	32.0
6	34.47765343973068	36.0	36.0	36.0	32.0	36.0
7	34.42348222727887	36.0	36.0	36.0	32.0	36.0
8	34.38249846742101	36.0	36.0	36.0	32.0	36.0
9	34.49796972247332	36.0	36.0	36.0	32.0	36.0
10	34.21530604823208	36.0	36.0	36.0	32.0	36.0
11	34.48072691000904	36.0	36.0	36.0	32.0	36.0
12	34.327609280674956	36.0	36.0	36.0	32.0	36.0
13	34.39877185873258	36.0	36.0	36.0	32.0	36.0
14	34.31369554149393	36.0	36.0	36.0	32.0	36.0
15	34.27879014577684	36.0	36.0	36.0	32.0	36.0
16	34.268696944193344	36.0	36.0	36.0	32.0	36.0
17	34.21433143189635	36.0	36.0	36.0	32.0	36.0
18	34.21805014390657	36.0	36.0	36.0	32.0	36.0
19	34.21738204336939	36.0	36.0	36.0	32.0	36.0
20	34.19876562451295	36.0	36.0	36.0	32.0	36.0
21	34.19167523871866	36.0	36.0	36.0	32.0	36.0
22	34.16381139407542	36.0	36.0	36.0	32.0	36.0
23	34.128634809804346	36.0	36.0	36.0	32.0	36.0
24	34.11498706399426	36.0	36.0	36.0	32.0	36.0
25	33.74607400018703	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	20.0
5	69.0
6	201.0
7	33.0
8	164.0
9	130.0
10	78.0
11	19.0
12	31.0
13	47.0
14	168.0
15	232.0
16	382.0
17	479.0
18	668.0
19	867.0
20	1259.0
21	1819.0
22	2720.0
23	3784.0
24	5335.0
25	7608.0
26	10342.0
27	13306.0
28	17866.0
29	23777.0
30	31343.0
31	42275.0
32	59995.0
33	86254.0
34	192833.0
35	458326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27858823138243	17.165303442141923	11.714483429340914	25.841624897134736
2	16.599769957056573	19.167024268803203	38.03449397306191	26.19871180107831
3	19.82970013091997	22.41682425552254	27.872446541011204	29.88102907254629
4	13.39431103407498	15.351770047146529	34.566084635136576	36.68783428364191
5	15.286598383914818	35.835763836196236	33.38970436908442	15.48793341080453
6	35.08651001087483	33.95016291453797	16.574656860604627	14.388670213982579
7	30.980533285626915	29.80770430897116	19.82867294338885	19.38308946201307
8	27.801834205848365	33.63053972500435	18.883874195571654	19.68375187357564
9	26.794833252019917	15.377181548755281	18.618101926451757	39.20988327277304
10	16.676238334529792	26.955169220845427	31.031347212251738	25.337245232373046
11	36.37213038339243	21.714923671111556	22.094591220096888	19.818354725399125
12	24.63766909642528	23.45188724199855	28.52608054202414	23.38436311955203
13	29.548587521835074	19.827753924070652	24.800688318457567	25.82297023563671
14	23.216637435478134	19.63306016215508	25.3011020470154	31.849200355351385
15	24.99776305905316	27.009033079674634	22.335803332313706	25.6574005289585
16	25.487560662252346	26.10195939725258	23.614924084409605	24.79555585608547
17	24.05511544803408	26.209382525968657	25.149830169934095	24.58567185606317
18	24.379522258143705	25.75341496802433	25.707126184264727	24.159936589567234
19	25.251686926424217	25.327505012937955	25.4116433284244	24.00916473221343
20	25.521528284050003	25.055093396176375	25.23386743681289	24.18951088296072
21	25.539829837772853	24.66208310930339	25.17063541999245	24.62745163293131
22	25.469386396635148	24.603418938539818	25.14301047980796	24.78418418501708
23	24.60040907961247	24.886473648472297	25.36355988690519	25.149557385010052
24	24.600479829782103	25.026648543923557	25.39000469014373	24.982866936150607
25	24.829112403636866	24.83181623902737	25.434147569095995	24.90492378823977
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	265.0
1	265.0
2	308.0
3	351.0
4	351.0
5	351.0
6	777.0
7	1203.0
8	1203.0
9	1203.0
10	1339.0
11	1475.0
12	1475.0
13	1475.0
14	2059.5
15	2644.0
16	2644.0
17	2644.0
18	4495.5
19	6347.0
20	6347.0
21	6347.0
22	9584.0
23	12821.0
24	12821.0
25	12821.0
26	18929.5
27	25038.0
28	25038.0
29	25038.0
30	32499.5
31	39961.0
32	39961.0
33	39961.0
34	48913.5
35	57866.0
36	57866.0
37	57866.0
38	68161.0
39	78456.0
40	78456.0
41	78456.0
42	89554.5
43	100653.0
44	100653.0
45	100653.0
46	112330.0
47	124007.0
48	124007.0
49	124007.0
50	128731.5
51	133456.0
52	133456.0
53	133456.0
54	127043.5
55	120631.0
56	120631.0
57	120631.0
58	109891.5
59	99152.0
60	99152.0
61	99152.0
62	85431.0
63	71710.0
64	71710.0
65	71710.0
66	58082.0
67	44454.0
68	44454.0
69	44454.0
70	33505.0
71	22556.0
72	22556.0
73	22556.0
74	16584.5
75	10613.0
76	10613.0
77	10613.0
78	7877.0
79	5141.0
80	5141.0
81	5141.0
82	3600.0
83	2059.0
84	2059.0
85	2059.0
86	1419.0
87	779.0
88	779.0
89	779.0
90	547.5
91	316.0
92	316.0
93	316.0
94	207.5
95	99.0
96	99.0
97	99.0
98	238.0
99	377.0
100	377.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.234219631557619E-4
2	1.0390366052596034E-4
3	0.0010390366052596034
4	0.011533306318381597
5	0.03657408850513803
6	0.05984850846295315
7	0.10328023856280458
8	0.107332481323317
9	0.11730723273380922
10	0.13091861226271004
11	0.1377762538574234
12	0.13424352939954076
13	0.12956786467587253
14	0.11720332907328326
15	0.13465914404164459
16	0.12104776451274378
17	0.12208680111800338
18	0.11159253140488139
19	0.09538356036283158
20	0.09153912492337105
21	0.09112351028126721
22	0.09995532142597384
23	0.0788628783392039
24	0.08717517118128072
25	0.08655174921812496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	962430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.82873828623252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.11974746552949	55.02250120684674
2	11.962989760970993	16.22869003235562
3	3.1774964124488907	6.465767176963157
4	1.3034687566229235	3.536505646290288
5	0.6925935178316138	2.348887222987083
6	0.41669389200491064	1.6958292567763638
7	0.2673078856685992	1.2691809633203104
8	0.17810759111918872	0.9664650547851811
9	0.1491476451550612	0.910484693030747
>10	0.684702109449547	7.9742574559304895
>50	0.03327189714910281	1.5490203577871386
>100	0.013703222331371816	1.483500277590995
>500	4.6190623102638535E-4	0.21221203417854567
>1k	3.0793748735092355E-4	0.3366986211572988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1940	0.20157310142036303	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1284	0.13341230011533306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1171098157788097E-4
2	0.0	1.0390366052596033E-4	0.0	0.0	3.1171098157788097E-4
3	0.0	1.0390366052596033E-4	0.0	0.0	3.1171098157788097E-4
4	0.0	1.0390366052596033E-4	0.0	0.0	3.1171098157788097E-4
5	0.0	1.0390366052596033E-4	0.0	0.0	3.1171098157788097E-4
6	0.0	1.0390366052596033E-4	0.0	0.0	3.1171098157788097E-4
7	0.0	1.0390366052596033E-4	0.0	0.0	4.156146421038413E-4
8	0.0	1.0390366052596033E-4	0.0	0.0	4.156146421038413E-4
9	0.0	1.0390366052596033E-4	0.0	0.0	4.156146421038413E-4
10	0.0	1.0390366052596033E-4	0.0	0.0	4.156146421038413E-4
11	0.0	1.0390366052596033E-4	0.0	0.0	5.195183026298016E-4
12	0.0	1.0390366052596033E-4	0.0	0.0	0.0010390366052596032
13	0.0	1.0390366052596033E-4	0.0	0.0	0.0012468439263115239
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	25	0.0060410327	18.994228	2
AGAACCG	45	6.755926E-4	14.778667	5
CCCGTTT	40	0.0052677793	14.253823	12
AAGTGCG	50	0.0014969673	13.302185	7
GGTATCA	375	0.0	13.168647	1
CCGACCT	55	0.0030644047	12.092266	9
TCTTATA	55	0.0030739694	12.087236	2
GTATCAA	1010	0.0	11.941295	1
TAAGCCT	65	8.0179836E-4	11.692404	4
GTATTAT	65	8.0425094E-4	11.6881485	1
CTTAGAC	100	1.9315066E-6	11.397722	3
GAATAAG	75	2.079107E-4	11.395945	1
CTTATAC	60	0.0058816364	11.08112	3
CCAACGA	60	0.005883914	11.080543	19
GTTTAGA	60	0.0058884704	11.079391	1
CCGCTCC	95	1.356899E-5	11.001235	9
CGCCGGT	70	0.0014884645	10.858926	7
CCGTCAA	70	0.0014929929	10.854974	18
AGGACTG	225	0.0	10.5561905	5
TAAACAA	100	2.3941693E-5	10.450629	5
>>END_MODULE