FastQCFastQC Report
Thu 2 Feb 2017
SRR4062034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962430
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG300.005742152729.3357242
GTATCAA12450.023.3320521
TCGAACG602.8706065E-422.00179344
GTACTAA654.9215474E-420.3135681
GGTATCA6050.018.9145621
CCTTATA1102.7930491E-618.0014672
GTATAAG2003.6379788E-1217.6050931
CGTCGTA1154.2719694E-617.21700510
TACCGTC1154.2719694E-617.2170057
GTATTAA1702.4556357E-916.8283981
TATCAAC17250.016.5810622
GTATATG1206.386217E-616.5047741
ACCGCTA800.001988252216.49962827
TCAACGC17400.016.4364134
ATCAACG17300.016.2770923
AACGCAG17950.016.177916
GTAATAC1105.620414E-515.9996413
ATACCGT1401.5856785E-615.7139336
CAACGCA18100.015.6792075
GTGTAGG2550.015.5339061
Produced by FastQC (version 0.11.5)