FastQCFastQC Report
Thu 2 Feb 2017
SRR4062033_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062033_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823077
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA51300.6232709697877598No Hit
GTCCTACAGTGGACATTTCTAAATT38010.46180369515853315No Hit
GTCCTAAAGTGTGTATTTCTCATTT35680.4334952865892256No Hit
CTTTAGGACGTGAAATATGGCGAGG29790.3619345456135939No Hit
CTGAAGGACCTGGAATATGGCGAGA16940.20581306487728368No Hit
ATTTAGAAATGTCCACTGTAGGACG15190.18455138462136592No Hit
GTCCTTCAGTGTGCATTTCTCATTT13140.15964484489300515No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11100.13485980048039248No Hit
GTATCAACGCAGAGTACTTTTTTTT9560.11614952185518487No Hit
TTTCTAAATTTTCCACCTTTTTCAG9550.11602802653943678No Hit
GCCATATTCCACGTCCTACAGTGGA9280.11274765301423804No Hit
TTCCAGGTCCTTCAGTGTGCATTTC9280.11274765301423804No Hit
GAATATGGCAAGAAAACTGAAAATC8570.10412148559612285No Hit
GTCCTACAGTGGACATTTCTAAATA8440.10254204649139752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCC402.75832E-416.6256413
GGTATCA4050.015.2391511
ACGGTTG400.005277084714.24968119
TCCAACG954.8858055E-914.00053818
AGCACCG551.9587552E-413.8178725
ACCGCTC500.001497377113.3013218
TAAGACC500.00150012713.2980824
CTAACAC1301.4551915E-1113.151953
CGTCTTA1550.012.87224615
TCTTACA905.408001E-712.664072
GGGTATA853.959407E-612.2878591
CAGCACC1551.8189894E-1212.2562984
TAGGACC2250.012.242684
TTACCCC550.00306964212.0891674
ACTGTTC2700.011.964158
CTTCTCG1603.6379788E-1211.8761810
TAGATTA1209.986252E-911.8747345
CTACACT1459.640644E-1111.7914034
TGCACTG658.0178695E-411.6920445
TTCTCGT1657.2759576E-1211.516294511
Produced by FastQC (version 0.11.5)