##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062033_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	823077
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277079787188804	32.0	32.0	32.0	32.0	32.0
2	30.951500284906516	32.0	32.0	32.0	32.0	32.0
3	30.95056720088157	32.0	32.0	32.0	32.0	32.0
4	30.952841593192375	32.0	32.0	32.0	32.0	32.0
5	30.933732809931513	32.0	32.0	32.0	32.0	32.0
6	34.38618744054323	36.0	36.0	36.0	32.0	36.0
7	34.373138843631885	36.0	36.0	36.0	32.0	36.0
8	34.28687352459126	36.0	36.0	36.0	32.0	36.0
9	34.47879724497222	36.0	36.0	36.0	32.0	36.0
10	34.24798651887976	36.0	36.0	36.0	32.0	36.0
11	34.44015079998591	36.0	36.0	36.0	32.0	36.0
12	34.29975567292003	36.0	36.0	36.0	32.0	36.0
13	34.35004501401448	36.0	36.0	36.0	32.0	36.0
14	34.27094670364984	36.0	36.0	36.0	32.0	36.0
15	34.18442988930562	36.0	36.0	36.0	32.0	36.0
16	34.277783245066985	36.0	36.0	36.0	32.0	36.0
17	34.172511198830726	36.0	36.0	36.0	32.0	36.0
18	34.21340166229891	36.0	36.0	36.0	32.0	36.0
19	34.179444936500474	36.0	36.0	36.0	32.0	36.0
20	34.1086083076067	36.0	36.0	36.0	32.0	36.0
21	34.1006989625515	36.0	36.0	36.0	32.0	36.0
22	34.03791990299814	36.0	36.0	36.0	32.0	36.0
23	33.99566869199358	36.0	36.0	36.0	32.0	36.0
24	33.98024000184673	36.0	36.0	36.0	32.0	36.0
25	33.36288099412327	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	9.0
5	50.0
6	158.0
7	36.0
8	150.0
9	112.0
10	62.0
11	19.0
12	30.0
13	33.0
14	98.0
15	198.0
16	373.0
17	528.0
18	676.0
19	798.0
20	1023.0
21	1385.0
22	1756.0
23	2379.0
24	3276.0
25	4643.0
26	6464.0
27	8955.0
28	13051.0
29	18603.0
30	27100.0
31	41307.0
32	65421.0
33	104939.0
34	228233.0
35	291211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.79747967361141	21.169818289618842	12.807811748239514	25.224890288530233
2	13.589786580550472	20.684895890284473	43.94185698977249	21.783460539392568
3	17.980244693646956	25.44109249517052	30.35161043410646	26.22705237707607
4	10.868070153268903	17.93394394640084	40.46996469007215	30.728021210258106
5	11.49485494555977	39.36334280877315	37.11646040671877	12.02534183894831
6	29.39754114802347	39.90060529140666	18.85691222362837	11.844941336941497
7	27.26765236364167	32.16289418858782	22.73325979322012	17.836193654550385
8	23.332534683497503	39.975140503587234	20.512125085591343	16.180199727323917
9	25.32826197221131	16.12172239771424	21.84427580452311	36.70573982555134
10	15.172575716287762	28.66221582061663	35.24335292743693	20.921855535658672
11	32.636483634129576	23.228039441109022	26.675182637152744	17.460294287608654
12	23.518141408833127	25.616452687332274	32.19762493887174	18.66778096496286
13	27.726580480043594	23.161519635271965	26.980313638862803	22.131586245821637
14	19.039950569593003	24.32428487677671	28.385143632794353	28.25062092083594
15	21.240218040530433	34.561930006605444	23.282432069134565	20.91541988372956
16	20.439874814965282	29.03181076771316	29.945155526471673	20.58315889084989
17	19.428823746218495	30.83398107026821	29.43182827247039	20.30536691104291
18	19.432886685583416	28.68368849876976	32.413320862710854	19.47010395293597
19	22.671471012791997	27.527176457568974	28.39983899035766	21.401513539281368
20	22.634786753832984	29.05114211222663	29.22637462059304	19.08769651334734
21	21.69309531337403	27.806503392757254	27.504438553396405	22.99596274047231
22	21.830388090923464	30.749668584216096	27.8683580020189	19.551585322841543
23	20.183239707205562	30.903188152039103	28.997349286252767	19.916222854502568
24	22.186349331426598	28.158395229270333	29.805751336417185	19.84950410288588
25	20.9870958641588	29.56739772268511	29.65932692260923	19.786179490546857
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	57.0
2	152.0
3	247.0
4	247.0
5	247.0
6	644.5
7	1042.0
8	1042.0
9	1042.0
10	1443.5
11	1845.0
12	1845.0
13	1845.0
14	3118.0
15	4391.0
16	4391.0
17	4391.0
18	7897.0
19	11403.0
20	11403.0
21	11403.0
22	17806.5
23	24210.0
24	24210.0
25	24210.0
26	37083.0
27	49956.0
28	49956.0
29	49956.0
30	65760.0
31	81564.0
32	81564.0
33	81564.0
34	91344.0
35	101124.0
36	101124.0
37	101124.0
38	104215.0
39	107306.0
40	107306.0
41	107306.0
42	108148.0
43	108990.0
44	108990.0
45	108990.0
46	110369.0
47	111748.0
48	111748.0
49	111748.0
50	99544.0
51	87340.0
52	87340.0
53	87340.0
54	72252.0
55	57164.0
56	57164.0
57	57164.0
58	46108.0
59	35052.0
60	35052.0
61	35052.0
62	27269.0
63	19486.0
64	19486.0
65	19486.0
66	14981.0
67	10476.0
68	10476.0
69	10476.0
70	7757.0
71	5038.0
72	5038.0
73	5038.0
74	3789.0
75	2540.0
76	2540.0
77	2540.0
78	1832.0
79	1124.0
80	1124.0
81	1124.0
82	793.0
83	462.0
84	462.0
85	462.0
86	308.0
87	154.0
88	154.0
89	154.0
90	115.5
91	77.0
92	77.0
93	77.0
94	52.5
95	28.0
96	28.0
97	28.0
98	140.5
99	253.0
100	253.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001093457841732912
2	3.64485947244304E-4
3	8.504672102367093E-4
4	0.009598129944100005
5	0.03013083830552913
6	0.06050466724255446
7	0.10327101838588613
8	0.10399999028037474
9	0.11469157806620765
10	0.12890653000873553
11	0.13267288479692665
12	0.12574765179928488
13	0.11967288601187982
14	0.11068223264652031
15	0.12489718458904818
16	0.11347662490872663
17	0.1195513906961317
18	0.10667288722683296
19	0.09306541186304562
20	0.09002802896934309
21	0.08954204770635069
22	0.10290653243864184
23	0.07982242244650258
24	0.08857008518036588
25	0.08565419760241143
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	823077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.825037547527735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.33991549842851	23.20184606890237
2	16.71154181938087	12.642293935903226
3	7.872373972827193	8.93318523331106
4	4.319079663837307	6.534774018216385
5	2.6274418260395387	4.969154286194519
6	1.7073145907445846	3.8747543100213515
7	1.1616483831145048	3.075757559583171
8	0.8064868211333528	2.440431543276428
9	0.5608566714384189	1.9092982190345658
>10	2.6457596665844725	16.87459498975968
>50	0.13926449638491709	3.6799393938961367
>100	0.09735598275631847	7.038196574466954
>500	0.008381640899396232	2.25107669715744
>1k	0.002256595626760524	1.9491609882488314
>5k	3.22370803822932E-4	0.6255361820279327
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	5130	0.6232709697877598	No Hit
GTCCTACAGTGGACATTTCTAAATT	3801	0.46180369515853315	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3568	0.4334952865892256	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2979	0.3619345456135939	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1694	0.20581306487728368	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1519	0.18455138462136592	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1314	0.15964484489300515	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1110	0.13485980048039248	No Hit
GTATCAACGCAGAGTACTTTTTTTT	956	0.11614952185518487	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	955	0.11602802653943678	No Hit
GCCATATTCCACGTCCTACAGTGGA	928	0.11274765301423804	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	928	0.11274765301423804	No Hit
GAATATGGCAAGAAAACTGAAAATC	857	0.10412148559612285	No Hit
GTCCTACAGTGGACATTTCTAAATA	844	0.10254204649139752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2149531574810133E-4	0.0
10	0.0	0.0	0.0	1.2149531574810133E-4	0.0
11	0.0	0.0	0.0	1.2149531574810133E-4	0.0
12	0.0	0.0	0.0	1.2149531574810133E-4	0.0
13	0.0	0.0	0.0	1.2149531574810133E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCCC	40	2.75832E-4	16.62564	13
GGTATCA	405	0.0	15.239151	1
ACGGTTG	40	0.0052770847	14.249681	19
TCCAACG	95	4.8858055E-9	14.000538	18
AGCACCG	55	1.9587552E-4	13.817872	5
ACCGCTC	50	0.0014973771	13.301321	8
TAAGACC	50	0.001500127	13.298082	4
CTAACAC	130	1.4551915E-11	13.15195	3
CGTCTTA	155	0.0	12.872246	15
TCTTACA	90	5.408001E-7	12.66407	2
GGGTATA	85	3.959407E-6	12.287859	1
CAGCACC	155	1.8189894E-12	12.256298	4
TAGGACC	225	0.0	12.24268	4
TTACCCC	55	0.003069642	12.089167	4
ACTGTTC	270	0.0	11.96415	8
CTTCTCG	160	3.6379788E-12	11.87618	10
TAGATTA	120	9.986252E-9	11.874734	5
CTACACT	145	9.640644E-11	11.791403	4
TGCACTG	65	8.0178695E-4	11.692044	5
TTCTCGT	165	7.2759576E-12	11.5162945	11
>>END_MODULE