FastQCFastQC Report
Thu 2 Feb 2017
SRR4062033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823077
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23540.28599997327103055No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC17790.21614016671587227No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA15010.1823644689379001No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA12250.14883176179142413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG250.002346165735.2189141
ACCGCGC250.00235317135.197528
TACGGCG353.2183054E-431.4263616
AATACGG353.2183054E-431.4263614
GTATCAA7400.025.8787671
CGCACAA450.001397418924.44569244
TAGGACC3800.024.3140774
GGTATCA3200.022.6996921
TAGGACG26550.021.8741664
CTGTAGG20000.021.6816461
TGTAGGA21200.021.5833852
TTAGGAC11950.021.3541453
GTCCTAC15350.021.2231251
GTAGGAC21500.021.0775853
GGACGTG25850.021.0197986
AGGACGT26550.020.9627425
ACGGGAT856.106826E-620.7069443
GACGTGG15150.020.619017
TATGACC1301.6243575E-920.3062634
CGTGGAA16200.020.2331439
Produced by FastQC (version 0.11.5)