##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	823077
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.143696154794753	32.0	32.0	32.0	32.0	32.0
2	31.20949072808498	32.0	32.0	32.0	32.0	32.0
3	31.451907901690852	32.0	32.0	32.0	32.0	32.0
4	31.553262938947388	32.0	32.0	32.0	32.0	32.0
5	31.518577241254462	32.0	32.0	32.0	32.0	32.0
6	35.118472512292286	36.0	36.0	36.0	36.0	36.0
7	35.14229288389786	36.0	36.0	36.0	36.0	36.0
8	35.072877750198344	36.0	36.0	36.0	36.0	36.0
9	35.206574840507024	36.0	36.0	36.0	36.0	36.0
10	35.04834177118301	36.0	36.0	36.0	36.0	36.0
11	35.21467007339532	36.0	36.0	36.0	36.0	36.0
12	35.13487559487144	36.0	36.0	36.0	36.0	36.0
13	35.172845310949036	36.0	36.0	36.0	36.0	36.0
14	35.13606624896577	36.0	36.0	36.0	36.0	36.0
15	35.102924756736	36.0	36.0	36.0	36.0	36.0
16	35.135859706929	36.0	36.0	36.0	36.0	36.0
17	35.126790081608405	36.0	36.0	36.0	36.0	36.0
18	35.121626530689106	36.0	36.0	36.0	36.0	36.0
19	35.106641298444735	36.0	36.0	36.0	36.0	36.0
20	35.099095224383625	36.0	36.0	36.0	36.0	36.0
21	35.082999524953316	36.0	36.0	36.0	36.0	36.0
22	35.036946725518995	36.0	36.0	36.0	36.0	36.0
23	35.03375382862114	36.0	36.0	36.0	36.0	36.0
24	35.02502317523148	36.0	36.0	36.0	36.0	36.0
25	34.98137598304897	36.0	36.0	36.0	36.0	36.0
26	34.95859196648673	36.0	36.0	36.0	36.0	36.0
27	34.908438700145915	36.0	36.0	36.0	32.0	36.0
28	34.86784954506079	36.0	36.0	36.0	32.0	36.0
29	34.82869281974833	36.0	36.0	36.0	32.0	36.0
30	34.791947776453476	36.0	36.0	36.0	32.0	36.0
31	34.78646833771324	36.0	36.0	36.0	32.0	36.0
32	34.75381161179331	36.0	36.0	36.0	32.0	36.0
33	34.78580983310189	36.0	36.0	36.0	32.0	36.0
34	34.76866076928404	36.0	36.0	36.0	32.0	36.0
35	34.76554198452879	36.0	36.0	36.0	32.0	36.0
36	34.6910167578489	36.0	36.0	36.0	32.0	36.0
37	34.61249069042143	36.0	36.0	36.0	32.0	36.0
38	34.619906764494694	36.0	36.0	36.0	32.0	36.0
39	34.46630752651332	36.0	36.0	36.0	32.0	36.0
40	34.5538740603856	36.0	36.0	36.0	32.0	36.0
41	34.533683968814586	36.0	36.0	36.0	32.0	36.0
42	34.521566026021866	36.0	36.0	36.0	32.0	36.0
43	34.533617146390924	36.0	36.0	36.0	32.0	36.0
44	34.43554369761274	36.0	36.0	36.0	32.0	36.0
45	34.41327117632979	36.0	36.0	36.0	32.0	36.0
46	34.30872931694118	36.0	36.0	36.0	32.0	36.0
47	34.33343052958593	36.0	36.0	36.0	32.0	36.0
48	34.29967913087111	36.0	36.0	36.0	32.0	36.0
49	34.25813745251052	36.0	36.0	36.0	32.0	36.0
50	33.889350571088734	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	8.0
22	23.0
23	90.0
24	280.0
25	717.0
26	1569.0
27	3416.0
28	6500.0
29	10868.0
30	17678.0
31	27919.0
32	44103.0
33	77376.0
34	172068.0
35	460461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.17659454749402	22.025008172804068	13.31334591560319	25.485051364098727
2	13.058031781825868	21.595190565810785	43.244083888079565	22.102693764283778
3	17.513692538720605	26.080094669265048	30.59061374648271	25.81559904553164
4	10.84698029467474	18.56497022757288	40.5310803241981	30.056969153554284
5	11.400877439170333	39.983865422068654	36.57689377786039	12.038363360900622
6	29.683078698782005	40.07131792364001	18.656258542660144	11.589344834917839
7	27.445913322811837	32.27936146921856	22.675035264015396	17.59968994395421
8	23.465969769535537	40.07073457282854	20.34487660328256	16.118419054353357
9	25.29739015912241	15.923662063209154	21.76369890058889	37.01524887707954
10	15.059830368240151	28.99971691591431	35.05407149027369	20.88638122557185
11	32.747118434848744	23.305838943379538	26.595689103206627	17.35135351856509
12	23.55332490155842	25.67694152551948	32.036735323669596	18.7329982492525
13	27.75961422807344	23.17583895552907	27.144361949125052	21.920184867272443
14	19.162241199790543	24.407922952530566	28.01524037240744	28.414595475271454
15	21.367259685302834	34.57659489938366	23.02105392326599	21.035091492047524
16	20.505860969635705	29.302782745616422	29.572139496909138	20.61921678783873
17	19.59950284116796	30.982034487660325	29.08731503856869	20.331147632603024
18	19.742477277228144	28.799511100473467	32.08658284259112	19.371428779707266
19	22.901624027885607	27.560118919615057	28.201371196133536	21.336885856365807
20	22.930352641010842	29.153965313003066	28.818272939890043	19.09740910609604
21	21.8334372118283	27.92181047459715	27.333165669797598	22.91158664377695
22	22.26717548904902	30.632249473621542	27.62171704469934	19.478857992630093
23	20.312437353977817	30.778286843150767	28.784791702355918	20.124484100515506
24	22.53803134844989	28.190277630969867	29.36065053926089	19.91104048131935
25	21.37632825238071	29.4896935147994	29.2773189287962	19.856659304023697
26	19.245460907429727	30.494294545313167	30.080478986042536	20.179765561214573
27	20.003547676150628	30.470650269599087	28.775784437365292	20.750017616884993
28	19.576313298771293	30.032281932930854	28.362660847737896	22.02874392055996
29	21.00275431219345	28.565631481047244	28.02971303881916	22.401901167940146
30	20.255191551609343	28.969902851283248	29.202325931757766	21.572579665349643
31	22.09026416854884	27.764973459219295	28.905212538863502	21.239549833368365
32	20.080211987087353	30.06430809567606	28.881539700802726	20.97394021643386
33	19.669798827322936	30.387304179211107	30.197892291580104	19.745004701885858
34	20.50955568785158	29.451565480457315	30.29268470482462	19.746194126866488
35	20.308866517469877	30.478183006631237	29.17210360186326	20.04084687403563
36	19.596586953590002	29.507324345109875	30.239941099070926	20.656147602229197
37	20.675695542139543	28.99012590773732	27.85910677554452	22.475071774578616
38	20.617998073084294	30.75301581747516	28.17014689998627	20.45883920945428
39	21.70656409122443	28.92710088704592	28.48120874434586	20.88512627738379
40	22.90648384051553	28.469875843936837	29.078081394571832	19.545558920975804
41	22.14565243011218	27.84845498980037	30.012429091607146	19.99346348848031
42	20.663444458214567	30.32618178018512	29.397913583576944	19.612460178023372
43	19.538252371978757	30.556213679170206	29.703406974328317	20.20212697452272
44	19.53947016713403	30.158604802612714	29.180537803200767	21.121387227052484
45	19.928364708197964	30.24963003822331	28.847541728529457	20.974463525049266
46	20.87952809958022	28.259229334613117	28.99040768842908	21.870834877377575
47	22.187298771671912	28.50899680464602	28.67265238679578	20.63105203688629
48	19.925204848319904	29.613096684519007	28.284863094254526	22.176835372906563
49	21.67705485693457	28.281392381993804	28.764473604276773	21.277079156794848
50	20.048477632250382	30.63130269968168	28.045585984010884	21.274633684057058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	4.5
4	7.0
5	11.0
6	15.0
7	32.0
8	49.0
9	54.0
10	59.0
11	108.5
12	158.0
13	331.5
14	505.0
15	755.5
16	1006.0
17	1402.5
18	1799.0
19	2144.5
20	2490.0
21	3370.5
22	4251.0
23	5332.0
24	6413.0
25	9045.5
26	11678.0
27	15939.5
28	20201.0
29	25065.5
30	29930.0
31	34447.5
32	38965.0
33	47917.0
34	56869.0
35	66817.5
36	76766.0
37	79040.5
38	81315.0
39	78026.5
40	74738.0
41	70490.0
42	66242.0
43	61105.0
44	55968.0
45	54316.5
46	52665.0
47	50384.0
48	48103.0
49	44704.0
50	41305.0
51	38814.5
52	36324.0
53	33097.5
54	29871.0
55	27149.5
56	24428.0
57	21620.5
58	18813.0
59	16131.0
60	13449.0
61	11354.5
62	9260.0
63	7716.5
64	6173.0
65	5095.5
66	4018.0
67	3443.5
68	2869.0
69	2500.0
70	2131.0
71	1773.5
72	1416.0
73	1226.0
74	1036.0
75	858.0
76	680.0
77	552.5
78	425.0
79	356.0
80	287.0
81	243.0
82	199.0
83	141.0
84	83.0
85	69.0
86	55.0
87	41.0
88	27.0
89	24.5
90	22.0
91	13.0
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027457941359070898
2	0.003401868840946837
3	1.2149531574810133E-4
4	0.0
5	0.0
6	2.4299063149620266E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.074765787405067E-4
17	0.0
18	4.859812629924053E-4
19	0.0
20	2.4299063149620266E-4
21	0.0
22	0.0
23	0.0
24	7.28971894488608E-4
25	3.64485947244304E-4
26	6.074765787405067E-4
27	3.64485947244304E-4
28	0.0019439250519696213
29	4.859812629924053E-4
30	0.001093457841732912
31	4.859812629924053E-4
32	9.719625259848107E-4
33	3.64485947244304E-4
34	8.504672102367093E-4
35	3.64485947244304E-4
36	0.0
37	0.0017009344204734185
38	0.0
39	9.719625259848107E-4
40	0.0
41	9.719625259848107E-4
42	0.0047383173141759515
43	0.0036448594724430403
44	0.0030373828937025333
45	0.002308410999213925
46	0.0026728969464582296
47	8.504672102367093E-4
48	0.003523364156694939
49	0.0032803735251987357
50	0.0020654203677177227
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	823077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.05538174741714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.39242418935994	26.659925991777328
2	15.938013592308486	13.40558491840113
3	7.452300591350855	9.402280387972882
4	4.136603634362477	6.958657799234685
5	2.4729596703128722	5.2000631490487335
6	1.6204497912979607	4.08891807453349
7	1.0777798394887863	3.1728509812559613
8	0.7703337467156228	2.5917343832834927
9	0.55502617953715	2.100765407422079
>10	2.4049815883190333	16.724661522872395
>50	0.111192766481717	3.2465110783217797
>100	0.06590218451131837	5.327952827011522
>500	8.709539801856948E-4	0.28264839803481073
>1k	0.0011612719735809266	0.8374450808297048
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2354	0.28599997327103055	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1779	0.21614016671587227	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1501	0.1823644689379001	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1225	0.14883176179142413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	1.2149531574810133E-4	0.0
21	0.0	0.0	0.0	1.2149531574810133E-4	0.0
22	0.0	0.0	0.0	1.2149531574810133E-4	0.0
23	0.0	0.0	0.0	1.2149531574810133E-4	0.0
24	0.0	0.0	0.0	2.4299063149620266E-4	0.0
25	0.0	0.0	0.0	2.4299063149620266E-4	0.0
26	0.0	0.0	0.0	2.4299063149620266E-4	0.0
27	0.0	0.0	0.0	3.64485947244304E-4	0.0
28	0.0	0.0	0.0	3.64485947244304E-4	0.0
29	0.0	0.0	0.0	3.64485947244304E-4	0.0
30	0.0	0.0	0.0	0.001093457841732912	0.0
31	0.0	0.0	0.0	0.004373831366931648	0.0
32	0.0	0.0	0.0	0.0075327095763822825	0.0
33	0.0	0.0	0.0	0.010813083101581019	0.0
34	0.0	0.0	0.0	0.01542990510000887	0.0
35	0.0	0.0	0.0	0.019925231782688617	0.0
36	0.0	0.0	0.0	0.02551401630710128	0.0
37	0.0	0.0	0.0	0.03268223993623926	0.0
38	0.0	0.0	0.0	0.04750466845750762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	25	0.0023461657	35.218914	1
ACCGCGC	25	0.002353171	35.19752	8
TACGGCG	35	3.2183054E-4	31.42636	16
AATACGG	35	3.2183054E-4	31.42636	14
GTATCAA	740	0.0	25.878767	1
CGCACAA	45	0.0013974189	24.445692	44
TAGGACC	380	0.0	24.314077	4
GGTATCA	320	0.0	22.699692	1
TAGGACG	2655	0.0	21.874166	4
CTGTAGG	2000	0.0	21.681646	1
TGTAGGA	2120	0.0	21.583385	2
TTAGGAC	1195	0.0	21.354145	3
GTCCTAC	1535	0.0	21.223125	1
GTAGGAC	2150	0.0	21.077585	3
GGACGTG	2585	0.0	21.019798	6
AGGACGT	2655	0.0	20.962742	5
ACGGGAT	85	6.106826E-6	20.70694	43
GACGTGG	1515	0.0	20.61901	7
TATGACC	130	1.6243575E-9	20.306263	4
CGTGGAA	1620	0.0	20.233143	9
>>END_MODULE