FastQCFastQC Report
Thu 2 Feb 2017
SRR4062032_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062032_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545784
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24680.15966008187431102No Hit
TATCAACGCAGAGTACTTTTTTTTT15890.10279573342718No Hit
GCGCAAGACGGACCAGAGCGAAAGC15630.1011137390476289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA653.3817087E-614.6113721
TATGCCG602.5662568E-514.2511665
CGACCAT2600.014.25116510
GGTATCA7700.014.0610741
TACTAAG707.2684834E-613.5703412
AACCGCG1153.765308E-1013.2193297
CGCCGGT2650.012.9075537
TCGCGTA1550.012.8728559
ACGGTAT2450.012.7977959
CGCATCG1650.012.66688213
ATTACCG2550.012.66606215
CCGACCA3000.012.351819
TGCCGTC701.0893988E-412.2160777
CGGTCCA2650.012.18967710
GCACCGA550.003065061312.0926826
TTACCGC2750.012.09033216
CGTCGTA2050.012.049767510
GACCATA3200.011.87635711
GTCCTAA1552.5465852E-1111.6419651
CCGGTCC2950.011.594929
Produced by FastQC (version 0.11.5)