##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062032_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1545784
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288587538750562	32.0	32.0	32.0	32.0	32.0
2	30.813642785796723	32.0	32.0	32.0	32.0	32.0
3	30.841809075524136	32.0	32.0	32.0	32.0	32.0
4	30.85941890975712	32.0	32.0	32.0	32.0	32.0
5	30.763651325152804	32.0	32.0	32.0	32.0	32.0
6	34.424345186649624	36.0	36.0	36.0	32.0	36.0
7	34.36441443306439	36.0	36.0	36.0	32.0	36.0
8	34.31867841820073	36.0	36.0	36.0	32.0	36.0
9	34.462126661939834	36.0	36.0	36.0	32.0	36.0
10	34.14023822215782	36.0	36.0	36.0	32.0	36.0
11	34.446105665474605	36.0	36.0	36.0	32.0	36.0
12	34.26824122904623	36.0	36.0	36.0	32.0	36.0
13	34.35594429752152	36.0	36.0	36.0	32.0	36.0
14	34.252526226173906	36.0	36.0	36.0	32.0	36.0
15	34.2017189982559	36.0	36.0	36.0	32.0	36.0
16	34.20228764174037	36.0	36.0	36.0	32.0	36.0
17	34.13610504443053	36.0	36.0	36.0	32.0	36.0
18	34.14178695082884	36.0	36.0	36.0	32.0	36.0
19	34.147175155131634	36.0	36.0	36.0	32.0	36.0
20	34.14386033236209	36.0	36.0	36.0	32.0	36.0
21	34.1136665924864	36.0	36.0	36.0	32.0	36.0
22	34.08312674992108	36.0	36.0	36.0	32.0	36.0
23	34.06110232736268	36.0	36.0	36.0	32.0	36.0
24	34.02368830315232	36.0	36.0	36.0	32.0	36.0
25	33.66969382526925	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	20.0
5	133.0
6	349.0
7	71.0
8	265.0
9	222.0
10	121.0
11	31.0
12	65.0
13	60.0
14	203.0
15	287.0
16	519.0
17	656.0
18	908.0
19	1178.0
20	1830.0
21	2633.0
22	3988.0
23	5713.0
24	8627.0
25	12136.0
26	16899.0
27	22118.0
28	29708.0
29	40285.0
30	53363.0
31	72892.0
32	105097.0
33	149895.0
34	328148.0
35	687363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71512579491127	17.654372901531275	11.991175918797751	25.639325384759697
2	16.725849263252684	19.3408380617953	37.471155357712384	26.462157317239637
3	19.122837661245455	22.923378488530062	28.2903776734076	29.66340617681688
4	13.04689041649818	15.515276991508289	35.204917104731095	36.23291548726243
5	15.13495603283365	36.1541607668433	33.27247994491485	15.438403255408206
6	34.91972658943036	34.722417323339265	16.520961196491676	13.8368948907387
7	30.816496097845608	30.09014038171236	20.126127888083197	18.967235632358836
8	28.180302122630053	33.08148891731296	18.97945371824555	19.75875524181144
9	26.848790876258477	14.809498888549207	18.763828365053293	39.577881870139024
10	16.642342102484477	26.59494321137366	30.71113883657974	26.051575849562127
11	36.92773027803223	21.547270920438393	21.926959369902452	19.598039431626926
12	24.457275155924886	23.647074675518173	28.442007861671886	23.453642306885055
13	29.239428189809686	19.60106205535824	25.119720896308394	26.039788858523682
14	23.57414054636626	19.658459557523386	24.961624305513276	31.80577559059708
15	25.20791037602012	27.177601260353413	21.915612809274375	25.698875554352092
16	25.7468177661016	25.920794567467503	23.48291711035285	24.849470556078046
17	24.05344605680522	26.307375713886895	24.934303297563147	24.70487493174474
18	24.840706577565115	25.220303548043205	25.816998120473627	24.121991753918053
19	25.53362064220332	25.145527015344367	25.140087358301795	24.180764984150525
20	25.672691764260986	24.641880752539308	24.75442234047651	24.931005142723194
21	26.480569777915342	24.402892921873107	24.458451319382434	24.658085980829114
22	25.8483439822916	24.440557312032826	24.933198456602636	24.777900249072935
23	24.582765711681006	24.48298947979094	25.440090232418406	25.494154576109647
24	24.70560885948161	25.06020569983986	25.18479377888465	25.049391661793873
25	24.87653485611867	24.735894346083143	25.089243914805003	25.298326882993184
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	266.0
2	368.0
3	470.0
4	470.0
5	470.0
6	961.0
7	1452.0
8	1452.0
9	1452.0
10	1797.5
11	2143.0
12	2143.0
13	2143.0
14	3032.5
15	3922.0
16	3922.0
17	3922.0
18	6693.0
19	9464.0
20	9464.0
21	9464.0
22	14962.0
23	20460.0
24	20460.0
25	20460.0
26	30665.0
27	40870.0
28	40870.0
29	40870.0
30	51800.0
31	62730.0
32	62730.0
33	62730.0
34	79025.0
35	95320.0
36	95320.0
37	95320.0
38	111867.5
39	128415.0
40	128415.0
41	128415.0
42	147226.0
43	166037.0
44	166037.0
45	166037.0
46	182701.0
47	199365.0
48	199365.0
49	199365.0
50	205062.5
51	210760.0
52	210760.0
53	210760.0
54	197727.0
55	184694.0
56	184694.0
57	184694.0
58	169540.5
59	154387.0
60	154387.0
61	154387.0
62	135162.0
63	115937.0
64	115937.0
65	115937.0
66	94413.0
67	72889.0
68	72889.0
69	72889.0
70	55275.5
71	37662.0
72	37662.0
73	37662.0
74	28398.5
75	19135.0
76	19135.0
77	19135.0
78	15351.0
79	11567.0
80	11567.0
81	11567.0
82	7886.0
83	4205.0
84	4205.0
85	4205.0
86	3168.5
87	2132.0
88	2132.0
89	2132.0
90	1428.0
91	724.0
92	724.0
93	724.0
94	458.5
95	193.0
96	193.0
97	193.0
98	389.0
99	585.0
100	585.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.056892812967401E-4
2	1.9407627456358715E-4
3	2.5876836608478286E-4
4	0.01028604255187012
5	0.03493372942144569
6	0.06475678361271692
7	0.1059009538201974
8	0.11055878440972347
9	0.12006852186333926
10	0.1343654740895235
11	0.1390879967705708
12	0.13533585546234145
13	0.1284138016695735
14	0.11890406421595771
15	0.13520647127929905
16	0.12291497389027187
17	0.12595550219176807
18	0.11502253872468597
19	0.10150189159675609
20	0.09483860617007292
21	0.09464452989550932
22	0.1072594877421425
23	0.08591109754014792
24	0.09671467682418759
25	0.09192746205161911
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1545784.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.74622645847619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43363715699806	41.84453208842015
2	13.975878375274576	15.302532049778042
3	4.344884342850038	7.135980665086669
4	1.7721759112785638	3.880797750524506
5	0.9320630732594988	2.55134680411239
6	0.5579156933658286	1.832626733624605
7	0.37277117952543204	1.4285470788044807
8	0.263889617781289	1.155756862007614
9	0.18993015899130333	0.935816354588905
>10	1.0151509768809375	9.91581075115727
>50	0.0687485797895045	2.5769869632558615
>100	0.06440010123178998	7.386522122698993
>500	0.006297307458100977	2.4128864539153296
>1k	0.0022575253151682745	1.6398573220252726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2468	0.15966008187431102	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1589	0.10279573342718	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1563	0.1011137390476289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	6.469209152119572E-5
10	0.0	0.0	0.0	0.0	6.469209152119572E-5
11	0.0	0.0	0.0	6.469209152119572E-5	6.469209152119572E-5
12	0.0	0.0	0.0	6.469209152119572E-5	1.2938418304239143E-4
13	0.0	0.0	0.0	6.469209152119572E-5	1.2938418304239143E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	65	3.3817087E-6	14.611372	1
TATGCCG	60	2.5662568E-5	14.251166	5
CGACCAT	260	0.0	14.251165	10
GGTATCA	770	0.0	14.061074	1
TACTAAG	70	7.2684834E-6	13.570341	2
AACCGCG	115	3.765308E-10	13.219329	7
CGCCGGT	265	0.0	12.907553	7
TCGCGTA	155	0.0	12.872855	9
ACGGTAT	245	0.0	12.797795	9
CGCATCG	165	0.0	12.666882	13
ATTACCG	255	0.0	12.666062	15
CCGACCA	300	0.0	12.35181	9
TGCCGTC	70	1.0893988E-4	12.216077	7
CGGTCCA	265	0.0	12.189677	10
GCACCGA	55	0.0030650613	12.092682	6
TTACCGC	275	0.0	12.090332	16
CGTCGTA	205	0.0	12.0497675	10
GACCATA	320	0.0	11.876357	11
GTCCTAA	155	2.5465852E-11	11.641965	1
CCGGTCC	295	0.0	11.59492	9
>>END_MODULE