FastQCFastQC Report
Thu 2 Feb 2017
SRR4062032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545784
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15510.10033743394937457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14900.027.027111
GGTATCA6950.024.380391
CCGTCGT4400.018.4987899
CGCAATA3850.018.28629336
CGTCGTA4350.018.20570610
ACCGTCG4500.018.0877068
TACCGTC4800.016.9572227
GTATAGG1954.9112714E-1116.9274461
CTAGCGG3900.016.92251829
ATACCGT4950.016.8877836
TCTAGCG3950.016.70830728
TAGGACG2400.016.498924
TACGAAT3900.016.36213941
TCAACGC24950.016.3115584
ATCAACG25100.016.2140793
GTCGTAG4900.016.16220911
GTATTAT1653.1022864E-816.004131
GTACCGT1105.6260193E-515.9989536
ATACGAA4000.015.95256840
CAACGCA25450.015.9046565
Produced by FastQC (version 0.11.5)