FastQCFastQC Report
Thu 2 Feb 2017
SRR4062031_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062031_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948444
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29070.14919597381295024No Hit
GCGCAAGACGGACCAGAGCGAAAGC24640.12645988286037474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.013.0010311
AAGACGG5300.012.7270695
CAAGACG5600.012.7232144
CGTTATT1750.012.4828272
GTATCAA20050.012.4085441
GGTATAT1155.3605618E-912.3858931
AATTCCG1405.2750693E-1112.2149135
GATAACG1251.4060788E-912.16250111
ATTCCGA1501.4551915E-1112.0342616
CGATAAC1353.7107384E-1011.96511610
GCGTTAT1750.011.9376421
CGTCGTA3450.011.842710510
CGCAAGA5500.011.7427392
GCGCAAG5500.011.5676741
TCTAGTA752.0787881E-411.3973642
ACGAACG1354.7257345E-911.25983815
CGAACGA1354.7311914E-911.25896916
GCGCGGT1104.956855E-711.22958211
GTATTAG1803.6379788E-1211.0784931
GCAAGAC6300.011.006243
Produced by FastQC (version 0.11.5)