##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062031_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1948444
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266415149729735	32.0	32.0	32.0	32.0	32.0
2	30.853807448404982	32.0	32.0	32.0	32.0	32.0
3	30.873954293785197	32.0	32.0	32.0	32.0	32.0
4	30.873203951460756	32.0	32.0	32.0	32.0	32.0
5	30.809302191902873	32.0	32.0	32.0	32.0	32.0
6	34.447202998905794	36.0	36.0	36.0	32.0	36.0
7	34.38211773086627	36.0	36.0	36.0	32.0	36.0
8	34.35503406821032	36.0	36.0	36.0	32.0	36.0
9	34.4712493661609	36.0	36.0	36.0	32.0	36.0
10	34.2052386417059	36.0	36.0	36.0	32.0	36.0
11	34.433076855172644	36.0	36.0	36.0	32.0	36.0
12	34.29083668814705	36.0	36.0	36.0	32.0	36.0
13	34.36734594373767	36.0	36.0	36.0	32.0	36.0
14	34.279801215739326	36.0	36.0	36.0	32.0	36.0
15	34.23696806272082	36.0	36.0	36.0	32.0	36.0
16	34.24296720870603	36.0	36.0	36.0	32.0	36.0
17	34.17451412511727	36.0	36.0	36.0	32.0	36.0
18	34.17199724498112	36.0	36.0	36.0	32.0	36.0
19	34.176053302019454	36.0	36.0	36.0	32.0	36.0
20	34.17187099039028	36.0	36.0	36.0	32.0	36.0
21	34.15320224753701	36.0	36.0	36.0	32.0	36.0
22	34.12082102436611	36.0	36.0	36.0	32.0	36.0
23	34.0955670268173	36.0	36.0	36.0	32.0	36.0
24	34.072897142540405	36.0	36.0	36.0	32.0	36.0
25	33.727974219428425	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	29.0
5	155.0
6	444.0
7	111.0
8	345.0
9	284.0
10	146.0
11	46.0
12	83.0
13	99.0
14	263.0
15	466.0
16	665.0
17	909.0
18	1299.0
19	1762.0
20	2654.0
21	3668.0
22	5438.0
23	7914.0
24	11121.0
25	15759.0
26	21660.0
27	27768.0
28	36808.0
29	48495.0
30	64370.0
31	87256.0
32	124025.0
33	177463.0
34	399195.0
35	907743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57700825437623	16.987653079083014	11.571421166164807	25.863917500375944
2	17.060144649349528	18.980967298900246	37.01189056248191	26.946997489268316
3	19.599514687677708	22.2966009661082	27.631289916630568	30.472594429583523
4	13.129192585478828	15.248859093197881	34.3112803358534	37.3106679854699
5	15.74781400158235	35.71282981223851	32.65646234578898	15.882893840390155
6	35.79905983595149	33.9501629853932	16.27116537509238	13.979611803562927
7	31.802048609255735	29.573918869483368	19.422585259385006	19.20144726187589
8	28.717319211951242	32.625294552285666	18.615653071102585	20.041733164660506
9	27.13650420399526	14.606892270630203	18.237251067057347	40.01935245831719
10	16.97750627281315	25.97797808292962	30.052968137912462	26.991547506344766
11	37.54342555476002	21.147467761392413	21.46310774574662	19.845998938100948
12	25.046024046600696	23.144416314355286	27.763832654407977	24.045726984636037
13	29.585790301907778	19.05180627661466	24.70462415447219	26.657779267005377
14	23.892373759719142	19.246867084851164	24.324227106351337	32.53653204907835
15	25.596418022748146	26.510619861533474	21.50548300107774	26.387479114640644
16	26.538830149410963	25.459987923791417	22.694690322331994	25.306491604465624
17	24.560067381905657	25.775017819307344	24.401273842798506	25.263640955988492
18	25.34324566523887	24.881405539615074	25.1005586477354	24.674790147410654
19	26.03857764954182	24.779388947458195	24.506168272698687	24.675865130301293
20	26.11963033383286	24.146255380775777	24.172867070499915	25.56124721489145
21	27.01265102267485	23.946167653534797	23.9694394527377	25.071741871052655
22	26.51255031815718	23.805915128971183	24.32041266668893	25.3611218861827
23	24.86536300107463	24.181544714277788	24.896235506360455	26.056856778287123
24	25.108358321975636	24.729739934889967	24.657920599049294	25.503981144085103
25	25.255858205083154	24.12387636610969	24.62382859015733	25.99643683864982
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	395.0
1	395.0
2	478.5
3	562.0
4	562.0
5	562.0
6	1226.5
7	1891.0
8	1891.0
9	1891.0
10	2223.0
11	2555.0
12	2555.0
13	2555.0
14	3629.5
15	4704.0
16	4704.0
17	4704.0
18	7732.0
19	10760.0
20	10760.0
21	10760.0
22	16758.5
23	22757.0
24	22757.0
25	22757.0
26	33684.0
27	44611.0
28	44611.0
29	44611.0
30	56342.5
31	68074.0
32	68074.0
33	68074.0
34	86933.0
35	105792.0
36	105792.0
37	105792.0
38	125239.0
39	144686.0
40	144686.0
41	144686.0
42	169697.0
43	194708.0
44	194708.0
45	194708.0
46	220364.0
47	246020.0
48	246020.0
49	246020.0
50	256378.0
51	266736.0
52	266736.0
53	266736.0
54	255975.5
55	245215.0
56	245215.0
57	245215.0
58	227333.0
59	209451.0
60	209451.0
61	209451.0
62	186831.5
63	164212.0
64	164212.0
65	164212.0
66	134407.0
67	104602.0
68	104602.0
69	104602.0
70	79811.5
71	55021.0
72	55021.0
73	55021.0
74	41237.0
75	27453.0
76	27453.0
77	27453.0
78	22259.0
79	17065.0
80	17065.0
81	17065.0
82	11654.0
83	6243.0
84	6243.0
85	6243.0
86	4650.5
87	3058.0
88	3058.0
89	3058.0
90	2006.0
91	954.0
92	954.0
93	954.0
94	586.0
95	218.0
96	218.0
97	218.0
98	459.5
99	701.0
100	701.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011804291013752513
2	4.1058403526095694E-4
3	4.1058403526095694E-4
4	0.010931799938822978
5	0.036285364116187074
6	0.06861885689298743
7	0.11024181346756695
8	0.11650322000529653
9	0.12512548474577664
10	0.14093297010332348
11	0.14745099166309117
12	0.13970121799754062
13	0.13436362553914816
14	0.12384240963558614
15	0.13872608091379582
16	0.12671649788241285
17	0.12938529411160904
18	0.11886407820804704
19	0.10428834495628306
20	0.09910472151111348
21	0.09658989429514012
22	0.10670052616344118
23	0.0886861516163667
24	0.09715444734362394
25	0.09535814218935725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1948444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.48548713276296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46765035482511	38.100210909283774
2	13.885121285255675	14.019942239672572
3	4.551297834638046	6.89323464803973
4	1.965839552930731	3.9698546981824374
5	1.0519645235481359	2.655447070885627
6	0.6577776421706081	1.9924934814014035
7	0.44652518200837205	1.5780128931516817
8	0.31030806004351347	1.253284285801555
9	0.22358715405661622	1.015911575025875
>10	1.2524962662457007	11.49779551218863
>50	0.09790308743250152	3.429017347754188
>100	0.0767988125569286	7.877042201686252
>500	0.00947130175034983	3.2729505466140987
>1k	0.0032589425377547803	2.4448025903121473
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2907	0.14919597381295024	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2464	0.12645988286037474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.566150220380981E-4
2	0.0	0.0	0.0	0.0	2.566150220380981E-4
3	0.0	0.0	0.0	5.132300440761962E-5	2.566150220380981E-4
4	0.0	0.0	0.0	5.132300440761962E-5	2.566150220380981E-4
5	0.0	0.0	0.0	5.132300440761962E-5	2.566150220380981E-4
6	5.132300440761962E-5	0.0	0.0	5.132300440761962E-5	2.566150220380981E-4
7	1.0264600881523923E-4	0.0	0.0	5.132300440761962E-5	3.079380264457177E-4
8	1.0264600881523923E-4	0.0	0.0	5.132300440761962E-5	3.079380264457177E-4
9	1.0264600881523923E-4	0.0	0.0	5.132300440761962E-5	3.079380264457177E-4
10	1.0264600881523923E-4	0.0	0.0	5.132300440761962E-5	3.079380264457177E-4
11	1.0264600881523923E-4	0.0	0.0	5.132300440761962E-5	3.5926103085333734E-4
12	1.5396901322285885E-4	0.0	0.0	5.132300440761962E-5	8.211680705219139E-4
13	1.5396901322285885E-4	0.0	0.0	5.132300440761962E-5	9.751370837447728E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	745	0.0	13.001031	1
AAGACGG	530	0.0	12.727069	5
CAAGACG	560	0.0	12.723214	4
CGTTATT	175	0.0	12.482827	2
GTATCAA	2005	0.0	12.408544	1
GGTATAT	115	5.3605618E-9	12.385893	1
AATTCCG	140	5.2750693E-11	12.214913	5
GATAACG	125	1.4060788E-9	12.162501	11
ATTCCGA	150	1.4551915E-11	12.034261	6
CGATAAC	135	3.7107384E-10	11.965116	10
GCGTTAT	175	0.0	11.937642	1
CGTCGTA	345	0.0	11.8427105	10
CGCAAGA	550	0.0	11.742739	2
GCGCAAG	550	0.0	11.567674	1
TCTAGTA	75	2.0787881E-4	11.397364	2
ACGAACG	135	4.7257345E-9	11.259838	15
CGAACGA	135	4.7311914E-9	11.258969	16
GCGCGGT	110	4.956855E-7	11.229582	11
GTATTAG	180	3.6379788E-12	11.078493	1
GCAAGAC	630	0.0	11.00624	3
>>END_MODULE