FastQCFastQC Report
Thu 2 Feb 2017
SRR4062031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948444
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23130.11871010919482419No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21240.10901006136178407No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19600.10059308863893444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22600.026.3889351
GGTATCA10550.026.283251
CGCAATA4400.021.0007636
TCTAGCG4750.020.37811728
GCGCAAT5000.018.92019835
TACCGTC5300.018.6784847
TAGCGGC5100.018.5487430
ATACGAA5250.018.44018240
CCCGTAA600.007400080618.3391741
ATACCGT5650.018.3001386
TACGAAT5350.018.09550741
CGTCGTA5350.018.0927210
CTAGCGG5300.017.84878729
CAATACG5350.017.68424438
TATGCGA750.001291608617.59928117
ACCGTCG5450.017.357098
CGAATGC5650.017.13424343
TCAACGC34850.017.1069054
ATCAACG35050.017.0720563
AATACGA5550.017.04697439
Produced by FastQC (version 0.11.5)