##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062031_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1948444
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295552759022073	32.0	32.0	32.0	32.0	32.0
2	31.441849496316035	32.0	32.0	32.0	32.0	32.0
3	31.567297289529492	32.0	32.0	32.0	32.0	32.0
4	31.642997694570642	32.0	32.0	32.0	32.0	32.0
5	31.615085165393513	32.0	32.0	32.0	32.0	32.0
6	35.25484386515599	36.0	36.0	36.0	36.0	36.0
7	35.25761171478369	36.0	36.0	36.0	36.0	36.0
8	35.19855946591229	36.0	36.0	36.0	36.0	36.0
9	35.28600206113186	36.0	36.0	36.0	36.0	36.0
10	35.169436740291225	36.0	36.0	36.0	36.0	36.0
11	35.29497332230231	36.0	36.0	36.0	36.0	36.0
12	35.208129666544174	36.0	36.0	36.0	36.0	36.0
13	35.2492291284738	36.0	36.0	36.0	36.0	36.0
14	35.20944199576688	36.0	36.0	36.0	36.0	36.0
15	35.181063966939774	36.0	36.0	36.0	36.0	36.0
16	35.195245026287644	36.0	36.0	36.0	36.0	36.0
17	35.17808825914422	36.0	36.0	36.0	36.0	36.0
18	35.174831814514555	36.0	36.0	36.0	36.0	36.0
19	35.16807514098429	36.0	36.0	36.0	36.0	36.0
20	35.16242961049946	36.0	36.0	36.0	36.0	36.0
21	35.160852967804054	36.0	36.0	36.0	36.0	36.0
22	35.13833243346999	36.0	36.0	36.0	36.0	36.0
23	35.11093108141676	36.0	36.0	36.0	36.0	36.0
24	35.09946603546214	36.0	36.0	36.0	36.0	36.0
25	35.07700298289301	36.0	36.0	36.0	36.0	36.0
26	35.036171427046405	36.0	36.0	36.0	36.0	36.0
27	35.01879859005442	36.0	36.0	36.0	36.0	36.0
28	35.002567689910514	36.0	36.0	36.0	36.0	36.0
29	34.98550433063511	36.0	36.0	36.0	36.0	36.0
30	34.96779430150418	36.0	36.0	36.0	36.0	36.0
31	34.96545551219332	36.0	36.0	36.0	36.0	36.0
32	34.9444387418884	36.0	36.0	36.0	36.0	36.0
33	34.926367398806434	36.0	36.0	36.0	32.0	36.0
34	34.911982587131064	36.0	36.0	36.0	32.0	36.0
35	34.897986803829106	36.0	36.0	36.0	32.0	36.0
36	34.87807963687948	36.0	36.0	36.0	32.0	36.0
37	34.874585566739405	36.0	36.0	36.0	32.0	36.0
38	34.85634177836263	36.0	36.0	36.0	32.0	36.0
39	34.84102802030748	36.0	36.0	36.0	32.0	36.0
40	34.83569761306971	36.0	36.0	36.0	32.0	36.0
41	34.80831473729807	36.0	36.0	36.0	32.0	36.0
42	34.768515800300136	36.0	36.0	36.0	32.0	36.0
43	34.77136166089454	36.0	36.0	36.0	32.0	36.0
44	34.72485378076044	36.0	36.0	36.0	32.0	36.0
45	34.71698545095471	36.0	36.0	36.0	32.0	36.0
46	34.70528431917982	36.0	36.0	36.0	32.0	36.0
47	34.67953505463847	36.0	36.0	36.0	32.0	36.0
48	34.65403214051828	36.0	36.0	36.0	32.0	36.0
49	34.646542574485075	36.0	36.0	36.0	32.0	36.0
50	34.214527079043584	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	23.0
21	55.0
22	116.0
23	362.0
24	902.0
25	2172.0
26	4606.0
27	8656.0
28	15134.0
29	24054.0
30	36353.0
31	54351.0
32	82169.0
33	139382.0
34	322207.0
35	1257895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28117292633991	17.525305316940237	11.945300675835245	26.248221080884605
2	16.57931177484405	19.438978348099493	36.75018245983574	27.23152741722072
3	18.854407190158696	22.724424296142402	27.929098186704138	30.492070326994764
4	12.9090766319569	15.60466485291076	34.49590262584022	36.99035588929212
5	15.28322086752301	36.190211266015346	32.54094036061596	15.98562750584569
6	35.96649834406072	34.00626449556338	16.139877984501457	13.887359175874456
7	31.878976249766485	29.552042552929414	19.43119740675123	19.137783790552874
8	28.782556747845973	32.69721890903716	18.51580030013693	20.004424042979938
9	27.32015906025526	14.350989815463006	18.133597886313385	40.195253237968345
10	17.003140974317915	25.97159779105336	30.154995791505	26.87026544312373
11	37.89690645458633	20.94635514287298	21.39589333847932	19.760845064061375
12	25.113013255705578	23.045363377135807	27.822457304392636	24.019166062765983
13	29.71247826470763	18.944963262993443	24.74990299952167	26.592655472777256
14	24.08065102204631	19.016302239120037	24.329362301405634	32.57368443742802
15	25.672844587783892	26.49852908269368	21.47354504414805	26.355081285374382
16	26.606779194757628	25.391186348817925	22.699894068666495	25.302140387757948
17	24.740151628684224	25.654932859245633	24.36610957256149	25.23880593950865
18	25.561666994279015	24.72643513455116	25.048538806153225	24.663359065016596
19	26.22324594560888	24.622480616574364	24.51424034472653	24.640033093090228
20	26.250141780163506	24.04139929451217	24.180279700827175	25.52817922449715
21	27.177327138988854	23.84384667970955	23.963583248992528	25.01524293230906
22	26.61056720131551	23.634294852713243	24.35014811819072	25.40498982778053
23	25.035682337127646	24.036474251492088	24.967268737140373	25.960574674239894
24	25.3659620637376	24.503368359896655	24.615972071815918	25.514697504549826
25	25.509792449344094	23.880181067931268	24.68954650900207	25.920479973722564
26	25.249957016785267	24.81940634406588	24.939656698837787	24.99097994031107
27	25.81666992569919	24.297550182685455	24.28199920859399	25.603780683021366
28	25.41030976090199	24.138911499964586	24.908412312423593	25.542366426709833
29	25.26435291466955	24.47957003485358	24.920487549764246	25.335589500712626
30	25.297305453606693	24.425931409959983	25.050797647526174	25.22596548890715
31	25.840353062480336	24.24184102436979	24.102652536366328	25.815153376783545
32	25.623875694471177	24.204421519945598	24.196004465144924	25.9756983204383
33	25.259194668142037	24.051099367237054	24.611394044155347	26.078311920465563
34	25.915547172716423	24.13328714217894	24.80721608478643	25.143949600318205
35	26.41941904233032	23.897450162643104	24.736019313932495	24.947111481094087
36	25.183454644181648	24.5004459982817	24.48309876937321	25.83300058816344
37	26.233149678839872	24.247371568026153	24.27395741645089	25.24552133668308
38	25.47511012639323	24.058337862590797	24.81961237767797	25.646939633338008
39	25.948664151129325	24.02685452447722	24.23989850258465	25.784582821808804
40	26.146428787718595	24.184604930503447	24.645742598445324	25.02322368333263
41	25.13164512784718	24.70601820962626	24.927120436045616	25.235216226480944
42	26.34753036171268	24.580694765087443	24.3665126553757	24.70526221782418
43	25.904365477543877	23.84236958870432	24.514013103361552	25.739251830390252
44	25.380474615522008	24.50522389132171	24.466781224786434	25.647520268369846
45	25.637858012436897	24.55614772356338	24.306968398621226	25.499025865378496
46	25.444867529020126	24.511327050193778	24.407856952590063	25.635948468196034
47	25.621659462516693	24.313980499010988	24.840819250939482	25.223540787532833
48	26.282803719418652	24.79540579838829	23.827365350198036	25.094425131995017
49	25.519601266495208	24.909706528896734	24.080038226845122	25.49065397776294
50	25.773723403217918	24.852507412480886	23.965421937863983	25.408347246437213
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	157.0
1	107.5
2	58.0
3	144.5
4	231.0
5	240.0
6	249.0
7	199.0
8	149.0
9	210.0
10	271.0
11	471.5
12	672.0
13	1280.0
14	1888.0
15	2365.5
16	2843.0
17	2929.0
18	3015.0
19	2985.5
20	2956.0
21	3459.0
22	3962.0
23	4888.0
24	5814.0
25	7562.0
26	9310.0
27	11708.0
28	14106.0
29	16578.0
30	19050.0
31	21625.0
32	24200.0
33	28151.0
34	32102.0
35	37373.0
36	42644.0
37	51345.0
38	60046.0
39	67276.0
40	74506.0
41	82456.0
42	90406.0
43	95541.5
44	100677.0
45	111451.0
46	122225.0
47	131551.0
48	140877.0
49	147665.5
50	154454.0
51	151386.0
52	148318.0
53	149331.0
54	150344.0
55	152694.5
56	155045.0
57	151246.5
58	147448.0
59	135761.5
60	124075.0
61	109410.0
62	94745.0
63	82287.0
64	69829.0
65	59201.0
66	48573.0
67	41294.0
68	34015.0
69	30283.0
70	26551.0
71	20839.5
72	15128.0
73	13329.0
74	11530.0
75	8501.5
76	5473.0
77	4772.5
78	4072.0
79	3415.0
80	2758.0
81	2203.5
82	1649.0
83	1355.5
84	1062.0
85	820.0
86	578.0
87	396.5
88	215.0
89	135.5
90	56.0
91	45.5
92	35.0
93	33.5
94	32.0
95	23.5
96	15.0
97	18.0
98	21.0
99	15.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0324361387856156
2	0.0035926103085333733
3	1.5396901322285887E-4
4	5.132300440761962E-5
5	0.0
6	1.5396901322285887E-4
7	0.0
8	0.0
9	0.0
10	2.0529201763047847E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.158760528914355E-4
17	0.0
18	1.5396901322285887E-4
19	5.132300440761962E-5
20	2.566150220380981E-4
21	0.0
22	0.0
23	5.132300440761962E-5
24	9.238140793371532E-4
25	2.0529201763047847E-4
26	4.619070396685766E-4
27	6.671990572990551E-4
28	0.0019502741674895453
29	3.592610308533373E-4
30	0.0013857211190057297
31	3.592610308533373E-4
32	9.751370837447727E-4
33	2.566150220380981E-4
34	9.751370837447727E-4
35	3.0793802644571775E-4
36	3.0793802644571775E-4
37	0.0020015971718971653
38	5.132300440761962E-5
39	0.0010264600881523924
40	1.0264600881523923E-4
41	0.001231752105782871
42	0.005234946449577201
43	0.004567747392278146
44	0.004516424387870526
45	0.002463504211565742
46	0.002822765242419079
47	0.0015396901322285886
48	0.003849225330571471
49	0.003849225330571471
50	0.0021042431807124042
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1948444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.419547637665794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67787512345441	38.39902557390122
2	14.667544093645482	15.083969645015353
3	4.680089315637052	7.219442265417905
4	1.9515582474502153	4.013929690897838
5	1.011210703815057	2.5997998478267945
6	0.6475739636540588	1.9978776163813161
7	0.42670814221742215	1.5358797752290032
8	0.3032693953094174	1.247518009532695
9	0.23106714511684534	1.0693231268251562
>10	1.2321590651791434	11.384178500234148
>50	0.09140248338585438	3.270699508642839
>100	0.0679082734386991	7.000526387111222
>500	0.008825829286912848	3.095053419319391
>1k	0.00280821840947227	2.0827766336652265
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2313	0.11871010919482419	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2124	0.10901006136178407	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1960	0.10059308863893444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5396901322285885E-4
2	0.0	0.0	0.0	0.0	2.0529201763047847E-4
3	0.0	0.0	0.0	5.132300440761962E-5	2.0529201763047847E-4
4	0.0	0.0	0.0	5.132300440761962E-5	2.0529201763047847E-4
5	5.132300440761962E-5	0.0	0.0	5.132300440761962E-5	2.566150220380981E-4
6	5.132300440761962E-5	0.0	0.0	5.132300440761962E-5	3.079380264457177E-4
7	5.132300440761962E-5	0.0	0.0	1.0264600881523923E-4	3.079380264457177E-4
8	5.132300440761962E-5	0.0	0.0	1.0264600881523923E-4	3.079380264457177E-4
9	5.132300440761962E-5	0.0	0.0	1.0264600881523923E-4	3.079380264457177E-4
10	5.132300440761962E-5	0.0	0.0	1.0264600881523923E-4	4.1058403526095694E-4
11	5.132300440761962E-5	0.0	0.0	1.0264600881523923E-4	4.1058403526095694E-4
12	1.5396901322285885E-4	0.0	0.0	1.0264600881523923E-4	0.0011291060969676316
13	1.5396901322285885E-4	0.0	0.0	1.0264600881523923E-4	0.0013343981145981102
14	2.0529201763047847E-4	0.0	0.0	1.0264600881523923E-4	0.0013343981145981102
15	2.0529201763047847E-4	0.0	0.0	1.0264600881523923E-4	0.001744982149859067
16	2.0529201763047847E-4	0.0	0.0	1.0264600881523923E-4	0.0019502741674895456
17	2.566150220380981E-4	0.0	0.0	2.0529201763047847E-4	0.0019502741674895456
18	2.566150220380981E-4	0.0	0.0	2.566150220380981E-4	0.0020015971718971653
19	2.566150220380981E-4	0.0	0.0	2.566150220380981E-4	0.0020529201763047848
20	2.566150220380981E-4	0.0	0.0	3.079380264457177E-4	0.002412181207158122
21	2.566150220380981E-4	0.0	0.0	3.079380264457177E-4	0.002566150220380981
22	2.566150220380981E-4	0.0	0.0	4.1058403526095694E-4	0.0026174732247886004
23	2.566150220380981E-4	0.0	0.0	6.671990572990551E-4	0.0026687962291962204
24	3.079380264457177E-4	0.0	0.0	7.698450661142943E-4	0.0026687962291962204
25	3.079380264457177E-4	0.0	0.0	9.238140793371532E-4	0.0026687962291962204
26	3.079380264457177E-4	0.0	0.0	0.0011804291013752513	0.0028740882468266987
27	3.079380264457177E-4	0.0	0.0	0.0013857211190057297	0.0029254112512343182
28	3.5926103085333734E-4	0.0	0.0	0.001744982149859067	0.0029254112512343182
29	4.1058403526095694E-4	0.0	0.0	0.002412181207158122	0.0029254112512343182
30	4.1058403526095694E-4	0.0	0.0	0.0035926103085333733	0.0029254112512343182
31	4.1058403526095694E-4	0.0	0.0	0.006466698555360072	0.0029254112512343182
32	4.1058403526095694E-4	0.0	0.0	0.00949475581540963	0.003079380264457177
33	4.619070396685766E-4	0.0	0.0	0.013087366123943003	0.003079380264457177
34	4.619070396685766E-4	0.0	0.0	0.016936591454514476	0.0031307032688647966
35	5.132300440761962E-4	0.0	0.0	0.02150433884679262	0.0031307032688647966
36	5.645530484838158E-4	0.0	0.0	0.026431347269924105	0.0031307032688647966
37	5.645530484838158E-4	0.0	0.0	0.03474567398395848	0.0031307032688647966
38	6.671990572990551E-4	0.0	0.0	0.04511292087429764	0.0031307032688647966
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2260	0.0	26.388935	1
GGTATCA	1055	0.0	26.28325	1
CGCAATA	440	0.0	21.00076	36
TCTAGCG	475	0.0	20.378117	28
GCGCAAT	500	0.0	18.920198	35
TACCGTC	530	0.0	18.678484	7
TAGCGGC	510	0.0	18.54874	30
ATACGAA	525	0.0	18.440182	40
CCCGTAA	60	0.0074000806	18.339174	1
ATACCGT	565	0.0	18.300138	6
TACGAAT	535	0.0	18.095507	41
CGTCGTA	535	0.0	18.09272	10
CTAGCGG	530	0.0	17.848787	29
CAATACG	535	0.0	17.684244	38
TATGCGA	75	0.0012916086	17.599281	17
ACCGTCG	545	0.0	17.35709	8
CGAATGC	565	0.0	17.134243	43
TCAACGC	3485	0.0	17.106905	4
ATCAACG	3505	0.0	17.072056	3
AATACGA	555	0.0	17.046974	39
>>END_MODULE