FastQCFastQC Report
Thu 2 Feb 2017
SRR4062030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169947
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13190.776124321111876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA12450.7325813341806564No Hit
TATCAACGCAGAGTACTTTTTTTTT8040.4730886688202793No Hit
GTCCTAAAGTGTGTATTTCTCATTT7740.4554361065508658No Hit
CTGTAGGACGTGGAATATGGCAAGA6860.4036552572272532No Hit
GTCCTACAGTGGACATTTCTAAATT6630.3901216261540363No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6080.35775859532677834No Hit
CTTTAGGACGTGAAATATGGCGAGG5780.340106033057365No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5570.32774923946877554No Hit
GGTATCAACGCAGAGTACTTTTTTT4880.2871483462491247No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4800.2824409963106145No Hit
GTCCTACAGTGTGCATTTCTCATTT3630.2135960034599022No Hit
GTATCAACGCAGAGTACATGGGGTG3420.20123920987131283No Hit
CCATAGGGTCTTCTCGTCTTATTAT3210.18888241628272345No Hit
CTGAAGGACCTGGAATATGGCGAGA3140.18476348508652699No Hit
CTGTAGGACCTGGAATATGGCGAGA3040.17887929766338917No Hit
GTCCTTCAGTGTGCATTTCTCATTT2940.17299511024025138No Hit
GTACATGGGGTGGTATCAACGCAAA2850.16769934155942734No Hit
ATTTAGAAATGTCCACTGTAGGACG2640.155342547970838No Hit
GAATATGGCAAGAAAACTGAAAATC2280.13415947324754188No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2270.1335710545052281No Hit
TTTCTAAATTTTCCACCTTTTTCAG2170.1276868670820903No Hit
GATTAAACCTTGTACCTTTTGCATA2090.12297951714358006No Hit
GATATACACTGTTCTACAAATCCCG1990.11709532972044225No Hit
TATCAACGCAGAGTACATGGGGTGG1950.11474165475118714No Hit
GTATTAGAGGCACTGCCTGCCCAGT1920.11297639852424579No Hit
GTCAGGATACCGCGGCCGTTAAACT1880.11062272355499068No Hit
ATTCCAGGTCCTTCAGTGTGCATTT1850.10885746732804934No Hit
GAAATATGGCGAGGAAAACTGAAAA1790.10532695487416666No Hit
GTGTATATCAATGAGTTACAATGAG1780.10473853613185288No Hit
GGTATCAACGCAGAGTACATGGGGT1760.10356169864722531No Hit
TTGTAGAACAGTGTATATCAATGAG1710.10061960493565641No Hit
GTTCATGCTAGTCCCTAATTAAGGA1710.10061960493565641No Hit
ATAAATAATCCACCTATAACTTCTC1700.10003118619334264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAA250.00600722619.0017669
ATGAACC250.00600722619.00176617
CAAAAGG350.002162420716.2824344
GAACATG456.7138E-414.77915313
GTATTAG551.9473465E-413.8153981
GGACATG655.3921947E-513.155076
ATTTAGA801.974142E-613.0598671
TTTAGAA801.974142E-613.0598672
TGAGTTA1051.9441359E-812.66784512
TTACAAT701.0789676E-412.21542316
GAGTTAC1103.7200152E-812.09203413
ATGTCCA951.0206222E-612.0011179
TGTCCAC951.0206222E-612.00111710
TAGTGTG657.952809E-411.6933967
AAAAGAA657.972504E-411.68995218
TTAGAAA907.3681294E-611.6087723
GTTACAA752.0513767E-411.4010615
AATGTCC1001.8941737E-611.401068
AAATGTC1001.8941737E-611.401067
AGTTACA1104.8628135E-711.22831814
Produced by FastQC (version 0.11.5)