##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062030_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169947
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04545534784374	32.0	32.0	32.0	32.0	32.0
2	30.856102196567164	32.0	32.0	32.0	32.0	32.0
3	30.948419213048773	32.0	32.0	32.0	32.0	32.0
4	30.869341618269225	32.0	32.0	32.0	32.0	32.0
5	30.98574849806116	32.0	32.0	32.0	32.0	32.0
6	34.55679711910184	36.0	36.0	36.0	32.0	36.0
7	34.43422949507788	36.0	36.0	36.0	32.0	36.0
8	34.32910848676352	36.0	36.0	36.0	32.0	36.0
9	34.30630726049886	36.0	36.0	36.0	32.0	36.0
10	34.23126621829159	36.0	36.0	36.0	32.0	36.0
11	34.462208806274894	36.0	36.0	36.0	32.0	36.0
12	34.31479225876303	36.0	36.0	36.0	32.0	36.0
13	34.39248706949814	36.0	36.0	36.0	32.0	36.0
14	34.34673162809582	36.0	36.0	36.0	32.0	36.0
15	34.33371580551584	36.0	36.0	36.0	32.0	36.0
16	34.35952385155372	36.0	36.0	36.0	32.0	36.0
17	34.27447969072711	36.0	36.0	36.0	32.0	36.0
18	34.337158055158376	36.0	36.0	36.0	32.0	36.0
19	34.21424914826387	36.0	36.0	36.0	32.0	36.0
20	34.12277945477119	36.0	36.0	36.0	32.0	36.0
21	34.083084726414704	36.0	36.0	36.0	32.0	36.0
22	34.094829564511286	36.0	36.0	36.0	32.0	36.0
23	34.15541904240734	36.0	36.0	36.0	32.0	36.0
24	34.131741072216634	36.0	36.0	36.0	32.0	36.0
25	33.78676293197291	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3.0
5	14.0
6	43.0
7	10.0
8	27.0
9	17.0
10	12.0
11	4.0
12	4.0
13	10.0
14	100.0
15	107.0
16	133.0
17	151.0
18	220.0
19	263.0
20	407.0
21	525.0
22	700.0
23	974.0
24	1159.0
25	1422.0
26	1779.0
27	2161.0
28	2689.0
29	3432.0
30	4774.0
31	6310.0
32	9247.0
33	13748.0
34	33512.0
35	85990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61372797081408	22.582600253023035	13.043043337550383	23.760628438612493
2	12.525669767633438	22.629407991903356	44.64803732928501	20.196884911178188
3	17.600193001347478	28.869588754140995	30.597186181574255	22.93303206293727
4	10.14565249374724	19.289686626452845	41.461232896866264	29.10342798293365
5	10.247112701757692	41.71660328934201	36.23868332136424	11.797600687536054
6	26.42807091922957	42.284795703863345	19.559316245355568	11.72781713155152
7	25.09793525963889	34.63933315660805	23.304762746310857	16.9579688374422
8	22.756110060614628	39.85897821054306	20.975371257238116	16.4095404716042
9	25.80447513226266	16.360508548468815	22.610729477194262	35.22428684207426
10	15.069381021123649	28.84364964793919	34.85372536310874	21.233243967828418
11	30.540136006317102	24.588975709790333	28.17004325330882	16.700845030583743
12	23.734915621759214	27.525454888281324	32.344795889506926	16.394833600452532
13	27.41826195063605	24.378531572776176	28.47766013233639	19.72554634425138
14	19.108524163284063	26.24704697156256	29.11612397711808	25.5283048880353
15	20.8735003653334	35.843330897777356	24.331204186013625	18.951964550875623
16	18.83296313535106	29.999587577844682	31.768289449652094	19.399159837152165
17	17.311737360287054	31.81831572621271	30.668206430477895	20.201740483022338
18	17.516171220529497	29.35032342441059	35.38816820425812	17.745337150801785
19	22.288386667373466	27.65713074055095	29.850570447464058	20.203912144611525
20	22.019672517375426	29.86570856402403	30.875839321474853	17.238779597125692
21	20.852033784500122	27.71554110565313	29.114982742575773	22.31744236727097
22	21.325903060863826	31.432173236881173	29.284384646198074	17.95753905605693
23	19.01811585667507	31.963650498244956	31.243963344248392	17.774270300831585
24	21.257936461192326	29.33810797121082	32.010295312923326	17.39366025467353
25	19.768537605277107	30.590729724954357	32.322869426939164	17.31786324282938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1322.0
1	1322.0
2	801.5
3	281.0
4	281.0
5	281.0
6	614.0
7	947.0
8	947.0
9	947.0
10	964.5
11	982.0
12	982.0
13	982.0
14	1002.0
15	1022.0
16	1022.0
17	1022.0
18	1985.0
19	2948.0
20	2948.0
21	2948.0
22	4695.5
23	6443.0
24	6443.0
25	6443.0
26	9795.5
27	13148.0
28	13148.0
29	13148.0
30	16990.5
31	20833.0
32	20833.0
33	20833.0
34	21700.0
35	22567.0
36	22567.0
37	22567.0
38	22381.5
39	22196.0
40	22196.0
41	22196.0
42	21055.5
43	19915.0
44	19915.0
45	19915.0
46	20554.0
47	21193.0
48	21193.0
49	21193.0
50	18490.5
51	15788.0
52	15788.0
53	15788.0
54	12174.0
55	8560.0
56	8560.0
57	8560.0
58	6866.5
59	5173.0
60	5173.0
61	5173.0
62	4092.0
63	3011.0
64	3011.0
65	3011.0
66	2394.5
67	1778.0
68	1778.0
69	1778.0
70	1363.5
71	949.0
72	949.0
73	949.0
74	722.5
75	496.0
76	496.0
77	496.0
78	343.5
79	191.0
80	191.0
81	191.0
82	137.0
83	83.0
84	83.0
85	83.0
86	56.0
87	29.0
88	29.0
89	29.0
90	18.0
91	7.0
92	7.0
93	7.0
94	8.5
95	10.0
96	10.0
97	10.0
98	42.5
99	75.0
100	75.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011768374846275603
2	0.0
3	0.0
4	0.012945212330903164
5	0.03824721825039571
6	0.07061024907765362
7	0.11297639852424579
8	0.10944588607036312
9	0.12297951714358006
10	0.136513148216797
11	0.14651626683613125
12	0.13827840444373835
13	0.13415947324754188
14	0.12121426091663871
15	0.14063207941299344
16	0.1282752858244041
17	0.13180579827828676
18	0.11827216720506982
19	0.09885434870871507
20	0.0982659299664013
21	0.09708909248177373
22	0.112387979781932
23	0.08885123008938081
24	0.09473541751251861
25	0.09238174254326349
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	169947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.6900210065491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69026863547411	28.056982471005664
2	14.352428393524283	11.392963688679412
3	5.277827195635415	6.284312167911173
4	2.5114155251141552	3.9871253979181747
5	1.4232342999466288	2.824409963106145
6	0.91324200913242	2.1747956715917316
7	0.6760362924746487	1.8782326254655863
8	0.5426080768546522	1.7228900774947482
9	0.4047322540473225	1.4457448498649579
>10	2.8375733855185907	21.554955368438396
>50	0.23275810947043823	6.133088551136531
>100	0.12453300124533001	8.287877985489594
>500	0.0103777501037775	2.7479155266053534
>1k	0.002965071458222143	1.5087056552925324
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1319	0.776124321111876	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1245	0.7325813341806564	No Hit
TATCAACGCAGAGTACTTTTTTTTT	804	0.4730886688202793	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	774	0.4554361065508658	No Hit
CTGTAGGACGTGGAATATGGCAAGA	686	0.4036552572272532	No Hit
GTCCTACAGTGGACATTTCTAAATT	663	0.3901216261540363	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	608	0.35775859532677834	No Hit
CTTTAGGACGTGAAATATGGCGAGG	578	0.340106033057365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	557	0.32774923946877554	No Hit
GGTATCAACGCAGAGTACTTTTTTT	488	0.2871483462491247	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	480	0.2824409963106145	No Hit
GTCCTACAGTGTGCATTTCTCATTT	363	0.2135960034599022	No Hit
GTATCAACGCAGAGTACATGGGGTG	342	0.20123920987131283	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	321	0.18888241628272345	No Hit
CTGAAGGACCTGGAATATGGCGAGA	314	0.18476348508652699	No Hit
CTGTAGGACCTGGAATATGGCGAGA	304	0.17887929766338917	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	294	0.17299511024025138	No Hit
GTACATGGGGTGGTATCAACGCAAA	285	0.16769934155942734	No Hit
ATTTAGAAATGTCCACTGTAGGACG	264	0.155342547970838	No Hit
GAATATGGCAAGAAAACTGAAAATC	228	0.13415947324754188	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	227	0.1335710545052281	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	217	0.1276868670820903	No Hit
GATTAAACCTTGTACCTTTTGCATA	209	0.12297951714358006	No Hit
GATATACACTGTTCTACAAATCCCG	199	0.11709532972044225	No Hit
TATCAACGCAGAGTACATGGGGTGG	195	0.11474165475118714	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	192	0.11297639852424579	No Hit
GTCAGGATACCGCGGCCGTTAAACT	188	0.11062272355499068	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	185	0.10885746732804934	No Hit
GAAATATGGCGAGGAAAACTGAAAA	179	0.10532695487416666	No Hit
GTGTATATCAATGAGTTACAATGAG	178	0.10473853613185288	No Hit
GGTATCAACGCAGAGTACATGGGGT	176	0.10356169864722531	No Hit
TTGTAGAACAGTGTATATCAATGAG	171	0.10061960493565641	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	171	0.10061960493565641	No Hit
ATAAATAATCCACCTATAACTTCTC	170	0.10003118619334264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGAA	25	0.006007226	19.001766	9
ATGAACC	25	0.006007226	19.001766	17
CAAAAGG	35	0.0021624207	16.282434	4
GAACATG	45	6.7138E-4	14.779153	13
GTATTAG	55	1.9473465E-4	13.815398	1
GGACATG	65	5.3921947E-5	13.15507	6
ATTTAGA	80	1.974142E-6	13.059867	1
TTTAGAA	80	1.974142E-6	13.059867	2
TGAGTTA	105	1.9441359E-8	12.667845	12
TTACAAT	70	1.0789676E-4	12.215423	16
GAGTTAC	110	3.7200152E-8	12.092034	13
ATGTCCA	95	1.0206222E-6	12.001117	9
TGTCCAC	95	1.0206222E-6	12.001117	10
TAGTGTG	65	7.952809E-4	11.693396	7
AAAAGAA	65	7.972504E-4	11.689952	18
TTAGAAA	90	7.3681294E-6	11.608772	3
GTTACAA	75	2.0513767E-4	11.40106	15
AATGTCC	100	1.8941737E-6	11.40106	8
AAATGTC	100	1.8941737E-6	11.40106	7
AGTTACA	110	4.8628135E-7	11.228318	14
>>END_MODULE