FastQCFastQC Report
Thu 2 Feb 2017
SRR4062030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169947
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.42836884440443196No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA3880.2283064720177467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.21536125968684353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.2135960034599022No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC3390.19947395364437148No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA3350.1971202786751164No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA3310.19476660370586124No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG3200.18829399754040965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.1835866476018994No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.15828464168240686No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA2090.12297951714358006No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT1990.11709532972044225No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC1930.11356481726655958No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1860.10944588607036312No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.1065037923587942No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.1041501173895391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGAC207.841144E-443.995883
GACTTGG404.0999657E-738.4963957
TCCGGCA250.002348202535.19670529
CCTAGAG250.002348202535.1967053
CCGGCAC250.002348202535.19670530
ACATATA250.002348202535.1967052
AAGTGTA250.002348202535.1967057
ATACATG250.002348202535.1967051
ACTTGGA451.0310177E-634.219028
TAGGACC1200.031.1637484
CGTCTCC300.005725682729.33922237
CCGTCTC300.005725682729.33922236
CTTGGAA602.9226612E-729.3305859
TCGTACT406.9988926E-427.5055232
CAAGACC407.010952E-427.4974254
CTGGATG407.010952E-427.4974253
TGTAGGA5700.026.6290862
GATTCCT508.2887425E-526.40529839
CCACGTC508.305368E-526.3975289
CTGTAGG5600.026.3189641
Produced by FastQC (version 0.11.5)