##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062030_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169947
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.092069880609838	32.0	32.0	32.0	32.0	32.0
2	31.455041865993515	32.0	32.0	32.0	32.0	32.0
3	31.58329949925565	32.0	32.0	32.0	32.0	32.0
4	31.688026267012656	32.0	32.0	32.0	32.0	32.0
5	31.684554596433006	32.0	32.0	32.0	32.0	32.0
6	35.31559250825257	36.0	36.0	36.0	36.0	36.0
7	35.29565099707556	36.0	36.0	36.0	36.0	36.0
8	35.28558315239457	36.0	36.0	36.0	36.0	36.0
9	35.29579221757371	36.0	36.0	36.0	36.0	36.0
10	35.217656092781866	36.0	36.0	36.0	36.0	36.0
11	35.336863845787214	36.0	36.0	36.0	36.0	36.0
12	35.264347119984464	36.0	36.0	36.0	36.0	36.0
13	35.2816995886953	36.0	36.0	36.0	36.0	36.0
14	35.25936909742449	36.0	36.0	36.0	36.0	36.0
15	35.261705119831475	36.0	36.0	36.0	36.0	36.0
16	35.27299099130906	36.0	36.0	36.0	36.0	36.0
17	35.26481785497832	36.0	36.0	36.0	36.0	36.0
18	35.263170282499836	36.0	36.0	36.0	36.0	36.0
19	35.19196573049245	36.0	36.0	36.0	36.0	36.0
20	35.1462808993392	36.0	36.0	36.0	36.0	36.0
21	35.10454435794689	36.0	36.0	36.0	36.0	36.0
22	35.10074905705896	36.0	36.0	36.0	36.0	36.0
23	35.11751899121491	36.0	36.0	36.0	36.0	36.0
24	35.119825592684776	36.0	36.0	36.0	36.0	36.0
25	35.09927801020318	36.0	36.0	36.0	36.0	36.0
26	35.08536190694746	36.0	36.0	36.0	36.0	36.0
27	35.051068862645415	36.0	36.0	36.0	36.0	36.0
28	35.00413069957104	36.0	36.0	36.0	36.0	36.0
29	34.969149205340486	36.0	36.0	36.0	36.0	36.0
30	34.90905399918798	36.0	36.0	36.0	36.0	36.0
31	34.91101931778731	36.0	36.0	36.0	36.0	36.0
32	34.856531742249054	36.0	36.0	36.0	36.0	36.0
33	34.85935615221216	36.0	36.0	36.0	36.0	36.0
34	34.782055582034396	36.0	36.0	36.0	32.0	36.0
35	34.76186693498561	36.0	36.0	36.0	32.0	36.0
36	34.727856331679874	36.0	36.0	36.0	32.0	36.0
37	34.634903822956566	36.0	36.0	36.0	32.0	36.0
38	34.594803085667884	36.0	36.0	36.0	32.0	36.0
39	34.43491206081896	36.0	36.0	36.0	32.0	36.0
40	34.484292161673935	36.0	36.0	36.0	32.0	36.0
41	34.42284947660153	36.0	36.0	36.0	32.0	36.0
42	34.40955709721266	36.0	36.0	36.0	32.0	36.0
43	34.40772123073664	36.0	36.0	36.0	32.0	36.0
44	34.269542857479095	36.0	36.0	36.0	32.0	36.0
45	34.2239462891372	36.0	36.0	36.0	32.0	36.0
46	33.992474124285806	36.0	36.0	36.0	32.0	36.0
47	33.95746909330556	36.0	36.0	36.0	32.0	36.0
48	33.879903734693755	36.0	36.0	36.0	32.0	36.0
49	33.776718624041614	36.0	36.0	36.0	32.0	36.0
50	33.468857938063046	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	5.0
22	16.0
23	41.0
24	113.0
25	243.0
26	505.0
27	1008.0
28	1844.0
29	2856.0
30	4040.0
31	5673.0
32	8373.0
33	13197.0
34	26474.0
35	105555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.22355697074213	22.742388303914588	13.553615886711828	24.480438838631454
2	12.157605785469245	22.68407642562506	44.61319383558017	20.54512395332553
3	17.087680276792177	28.92136960346461	31.280928760142867	22.710021359600347
4	9.767162703666438	19.027108451458396	42.110187293685684	29.09554155118949
5	10.006649131788144	41.434682577509456	36.44842215514249	12.11024613555991
6	26.450754945688633	42.04923917008932	19.636825815258966	11.863180068963082
7	24.962488305177498	34.150058547664855	23.597356823009527	17.290096324148116
8	22.814759895732198	39.58469405167494	21.03891213142921	16.561633921163658
9	25.936909742449117	15.588977740118978	22.902434288336952	35.57167822909496
10	15.148252102125955	28.42768627866335	34.91853342512666	21.50552819408404
11	30.621899768751433	24.094570660264672	28.714834624912474	16.56869494607142
12	24.026314086156273	27.027838090698864	32.466592525905135	16.479255297239728
13	27.68098289466716	23.86567576950461	28.793094317640204	19.660247018188024
14	19.29072004801497	25.56208700359524	29.658069868841462	25.48912307954833
15	20.89710321453159	35.23922163968767	24.881286518738193	18.98238862704255
16	18.824325517079053	29.47188796375298	32.176880755538555	19.526905763629408
17	17.27009008690945	31.38213678382084	30.992603576409117	20.3551695528606
18	17.424255797395656	28.158190494683637	36.43076959287308	17.986784115047634
19	22.27635674651509	26.700088851230092	31.069686431652222	19.953867970602598
20	22.102184798790212	29.03669967695811	31.653986242769804	17.207129281481873
21	20.72469652303365	26.541215790805367	30.555408450869976	22.178679235291003
22	21.37784132700195	30.443020471088044	30.402419577868393	17.776718624041614
23	18.783503092140492	31.128528305883602	32.24358182256821	17.8443867794077
24	21.494012768836974	28.462737944629147	32.53699726382065	17.50625202271323
25	19.627295568618454	29.292073411122292	33.63813424185187	17.442496778407385
26	16.997063790475856	30.321806210171403	34.60196414176184	18.079165857590894
27	17.634909707143994	31.33270960946648	32.04116577521227	18.991214908177255
28	17.299714613551444	30.179764041307482	32.016240548412725	20.504280796728352
29	18.998976145363823	29.099243289044757	30.599719911030565	21.30206065456086
30	18.30875689925035	28.637331858355008	32.41441399032634	20.639497252068303
31	20.97359765103238	27.616845251754956	32.046461543893095	19.363095553319564
32	18.115165993903943	30.71916961858473	32.09195862215057	19.073705765360764
33	17.718465168552548	31.492171088633512	33.177990785362496	17.61137295745144
34	18.671923269293007	29.86142575539145	34.009238282973904	17.457412692341638
35	18.30816847704565	31.510009061701954	32.13491344309369	18.04690901815871
36	17.124263001188613	30.199004389629646	33.301754675014415	19.374977934167323
37	19.194082721853796	29.553438505851965	30.005354736588153	21.24712403570609
38	18.95002559621529	32.09471188076283	30.732522492306423	18.22274003071546
39	20.1300420724352	29.962634970137398	30.46338521286299	19.44393774456442
40	21.377252908259635	29.359153147746063	32.05822991873937	17.205364025254934
41	20.75070904875666	28.636155013945608	32.53974791992751	18.073388017370224
42	19.22477153297987	32.03540135461965	31.391044916645583	17.348782195754897
43	17.206072731552315	32.24079086736496	32.363775450158876	18.189360950923856
44	17.21696864132002	31.52344692444847	31.59053060840194	19.66905382582957
45	17.844323357380752	31.999152652081296	31.181226536112323	18.975297454425625
46	19.33318033905885	29.291930728900027	30.587674545871803	20.787214386169317
47	21.240276323067327	30.214303366951857	29.53173360949949	19.01368670048133
48	18.214449635758083	31.880877005095858	28.81453672429417	21.09013663485189
49	19.925973437527585	29.924266942844195	30.043133124238697	20.10662649538952
50	17.75055312338182	32.95732711952172	28.893047121404695	20.399072635691756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	603.0
1	336.5
2	70.0
3	169.0
4	268.0
5	239.5
6	211.0
7	152.5
8	94.0
9	144.0
10	194.0
11	309.0
12	424.0
13	737.0
14	1050.0
15	1399.5
16	1749.0
17	1791.5
18	1834.0
19	1766.0
20	1698.0
21	1790.5
22	1883.0
23	1873.5
24	1864.0
25	2322.0
26	2780.0
27	3996.5
28	5213.0
29	6445.0
30	7677.0
31	8768.0
32	9859.0
33	11923.5
34	13988.0
35	16011.5
36	18035.0
37	18341.0
38	18647.0
39	17410.0
40	16173.0
41	14894.0
42	13615.0
43	11467.0
44	9319.0
45	8623.5
46	7928.0
47	7279.5
48	6631.0
49	5911.0
50	5191.0
51	5139.0
52	5087.0
53	4506.5
54	3926.0
55	3716.0
56	3506.0
57	3271.5
58	3037.0
59	2556.5
60	2076.0
61	1805.0
62	1534.0
63	1341.5
64	1149.0
65	983.0
66	817.0
67	693.0
68	569.0
69	528.0
70	487.0
71	398.5
72	310.0
73	255.5
74	201.0
75	155.5
76	110.0
77	90.5
78	71.0
79	53.0
80	35.0
81	28.0
82	21.0
83	13.5
84	6.0
85	4.0
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025890424661806327
2	0.0023536749692551207
3	0.0
4	0.0
5	0.0
6	5.884187423137802E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0011768374846275603
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0011768374846275603
25	0.0
26	0.0
27	0.0
28	0.0011768374846275603
29	5.884187423137802E-4
30	5.884187423137802E-4
31	0.0
32	5.884187423137802E-4
33	0.0
34	0.0011768374846275603
35	5.884187423137802E-4
36	5.884187423137802E-4
37	0.0023536749692551207
38	0.0
39	0.0011768374846275603
40	0.0
41	5.884187423137802E-4
42	0.005884187423137802
43	0.004118931196196461
44	0.005884187423137802
45	0.002942093711568901
46	0.003530512453882681
47	5.884187423137802E-4
48	0.002942093711568901
49	0.004707349938510241
50	0.0017652562269413405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	169947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.80137336934456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79272991370927	35.27393834548418
2	13.779435478907395	13.173518802920912
3	4.614892228910472	6.617945594803086
4	2.124647635929441	4.0624429969343385
5	1.2125016925785048	2.8979623058953674
6	0.7952041557418417	2.280711045208212
7	0.5305464258896808	1.7752593455606747
8	0.396371111685562	1.5157666802002978
9	0.3335918362322587	1.4351533125033098
>10	2.189888843753462	19.662600693157277
>50	0.15879463791129658	5.192206982176796
>100	0.07016507256545662	5.684125050751117
>500	0.0012309661853588882	0.42836884440443196
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	728	0.42836884440443196	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	388	0.2283064720177467	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	366	0.21536125968684353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	363	0.2135960034599022	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	339	0.19947395364437148	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	335	0.1971202786751164	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	331	0.19476660370586124	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	320	0.18829399754040965	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	312	0.1835866476018994	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	269	0.15828464168240686	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	209	0.12297951714358006	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	199	0.11709532972044225	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	193	0.11356481726655958	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	186	0.10944588607036312	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.1065037923587942	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	177	0.1041501173895391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0011768374846275603	0.0
22	0.0	0.0	0.0	0.0011768374846275603	0.0
23	0.0	0.0	0.0	0.002942093711568901	0.0
24	0.0	0.0	0.0	0.004707349938510241	0.0
25	0.0	0.0	0.0	0.005884187423137802	0.0
26	0.0	0.0	0.0	0.006472606165451582	0.0
27	0.0	0.0	0.0	0.006472606165451582	0.0
28	0.0	0.0	0.0	0.008237862392392922	0.0
29	0.0	0.0	0.0	0.010591537361648043	0.0
30	0.0	0.0	0.0	0.021183074723296086	0.0
31	0.0	0.0	0.0	0.04236614944659217	0.0
32	0.0	0.0	0.0	0.06590289913914338	0.0
33	0.0	0.0	0.0	0.08237862392392922	0.0
34	0.0	0.0	0.0	0.10356169864722531	0.0
35	0.0	0.0	0.0	0.13298263576291433	0.0
36	0.0	0.0	0.0	0.1694645977863687	0.0
37	0.0	0.0	0.0	0.2165380971714711	0.0
38	0.0	0.0	0.0	0.2771452276297905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAGAC	20	7.841144E-4	43.99588	3
GACTTGG	40	4.0999657E-7	38.496395	7
TCCGGCA	25	0.0023482025	35.196705	29
CCTAGAG	25	0.0023482025	35.196705	3
CCGGCAC	25	0.0023482025	35.196705	30
ACATATA	25	0.0023482025	35.196705	2
AAGTGTA	25	0.0023482025	35.196705	7
ATACATG	25	0.0023482025	35.196705	1
ACTTGGA	45	1.0310177E-6	34.21902	8
TAGGACC	120	0.0	31.163748	4
CGTCTCC	30	0.0057256827	29.339222	37
CCGTCTC	30	0.0057256827	29.339222	36
CTTGGAA	60	2.9226612E-7	29.330585	9
TCGTACT	40	6.9988926E-4	27.50552	32
CAAGACC	40	7.010952E-4	27.497425	4
CTGGATG	40	7.010952E-4	27.497425	3
TGTAGGA	570	0.0	26.629086	2
GATTCCT	50	8.2887425E-5	26.405298	39
CCACGTC	50	8.305368E-5	26.397528	9
CTGTAGG	560	0.0	26.318964	1
>>END_MODULE