FastQCFastQC Report
Thu 2 Feb 2017
SRR4062029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567957
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22300.14222328801108702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT456.7669374E-414.7764624
GGTATCA5700.014.4945421
GTATCAA14650.012.3161821
GAACCGC550.003065375412.0925356
TAGGACC4900.011.8254774
AGGACGT8050.011.6829355
GCACCGT907.4493873E-611.6126736
GGTTCTA907.454686E-611.61193113
TAGGACG7900.011.5432764
TGTAGGA10150.011.4155152
TTAGGAC5500.011.3982573
GGACGTG8200.011.3551856
AGAACCG855.3240525E-511.1761895
GTAGGAC9350.011.1747613
CCGAATA600.005872606811.08411514
AGTACCG600.00587679711.0830545
TGGCGAG7200.011.08234718
GACGTGA5150.011.069457
TACACCG700.001491726410.856875
GTCCTAA3850.010.8530571
Produced by FastQC (version 0.11.5)