##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062029_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1567957
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2890079256	32.0	32.0	32.0	32.0	32.0
2	30.9912625154899	32.0	32.0	32.0	32.0	32.0
3	30.999545905914513	32.0	32.0	32.0	32.0	32.0
4	30.983989356850984	32.0	32.0	32.0	32.0	32.0
5	30.972714175197407	32.0	32.0	32.0	32.0	32.0
6	34.450819123228506	36.0	36.0	36.0	32.0	36.0
7	34.4341400944031	36.0	36.0	36.0	32.0	36.0
8	34.41200300773554	36.0	36.0	36.0	32.0	36.0
9	34.53537310015517	36.0	36.0	36.0	32.0	36.0
10	34.34052081785406	36.0	36.0	36.0	32.0	36.0
11	34.48449287831235	36.0	36.0	36.0	32.0	36.0
12	34.39622132494705	36.0	36.0	36.0	32.0	36.0
13	34.42442235341913	36.0	36.0	36.0	32.0	36.0
14	34.374932475826824	36.0	36.0	36.0	32.0	36.0
15	34.31994372294648	36.0	36.0	36.0	32.0	36.0
16	34.33612210028719	36.0	36.0	36.0	32.0	36.0
17	34.27760391388284	36.0	36.0	36.0	32.0	36.0
18	34.26619990216568	36.0	36.0	36.0	32.0	36.0
19	34.260151266903364	36.0	36.0	36.0	32.0	36.0
20	34.223142598936064	36.0	36.0	36.0	32.0	36.0
21	34.20625565624567	36.0	36.0	36.0	32.0	36.0
22	34.168335611244444	36.0	36.0	36.0	32.0	36.0
23	34.141225173904644	36.0	36.0	36.0	32.0	36.0
24	34.10833460356375	36.0	36.0	36.0	32.0	36.0
25	33.62528819349	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	20.0
5	127.0
6	375.0
7	47.0
8	243.0
9	209.0
10	99.0
11	23.0
12	58.0
13	66.0
14	234.0
15	419.0
16	721.0
17	985.0
18	1400.0
19	1721.0
20	2126.0
21	2710.0
22	3570.0
23	4691.0
24	6456.0
25	9021.0
26	12760.0
27	17110.0
28	24296.0
29	33394.0
30	48202.0
31	71648.0
32	110409.0
33	173741.0
34	400107.0
35	640969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2685379943901	17.68203160576093	12.201152848766089	25.848277551082884
2	15.938541564357116	19.17594521267843	39.090241167789365	25.79527205517509
3	19.562077034491598	22.381623868663926	28.23284205397249	29.823457042871986
4	12.944932213714166	15.643435525690542	35.513252689286546	35.898379571308745
5	14.659583983830455	36.31786835575807	34.03943240710199	14.983115253309482
6	34.61211517843468	34.88008628171747	16.723081635778833	13.784716904069025
7	30.582394108130817	30.112323971087147	19.92553445499827	19.379747465783772
8	27.081753200487256	34.958476024117616	18.963637152977288	18.99613362241784
9	26.43831181891494	15.254772982819754	18.884417227114234	39.422497971151074
10	16.00669750191097	27.55005405640129	31.966842001408725	24.47640644027901
11	36.10542019404182	21.747193852787756	22.583801237412573	19.56358471575785
12	24.31345777932177	23.560740698640487	29.541479264359065	22.58432225767868
13	29.614391651697712	20.4367890811031	24.99931354600423	24.949505721194953
14	22.492657387306856	20.258204571574513	25.72787638871153	31.5212616524071
15	24.36365656644352	28.558200542933026	22.163570867064426	24.91457202355903
16	24.336701041854894	26.63852545319762	24.88128524169795	24.143488263249537
17	23.26563868888134	26.92305236352305	26.01120524733534	23.800103700260273
18	23.35688409729662	26.311179100303782	26.933871855411162	23.398064946988434
19	24.8974615141543	25.601394204221556	25.774456825312726	23.726687456311424
20	25.132057790723433	25.544301518816198	25.912818558500245	23.410822131960124
21	24.987137405700906	25.13938200819887	25.254666600279087	24.618813985821145
22	24.82521924602478	25.87438257753013	25.60171757881653	23.69868059762856
23	23.89651206282704	25.85124023912469	25.948386240245508	24.303861457802764
24	24.134664487794915	25.55254825860484	26.273044122153	24.039743131447246
25	23.989048853755342	25.826921333798037	26.04893163129694	24.13509818114968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	64.0
2	219.5
3	375.0
4	375.0
5	375.0
6	779.5
7	1184.0
8	1184.0
9	1184.0
10	1604.0
11	2024.0
12	2024.0
13	2024.0
14	3042.0
15	4060.0
16	4060.0
17	4060.0
18	7227.5
19	10395.0
20	10395.0
21	10395.0
22	17040.0
23	23685.0
24	23685.0
25	23685.0
26	35770.5
27	47856.0
28	47856.0
29	47856.0
30	63194.5
31	78533.0
32	78533.0
33	78533.0
34	94102.5
35	109672.0
36	109672.0
37	109672.0
38	127228.5
39	144785.0
40	144785.0
41	144785.0
42	161617.0
43	178449.0
44	178449.0
45	178449.0
46	191706.5
47	204964.0
48	204964.0
49	204964.0
50	207300.0
51	209636.0
52	209636.0
53	209636.0
54	196031.0
55	182426.0
56	182426.0
57	182426.0
58	164230.0
59	146034.0
60	146034.0
61	146034.0
62	124789.5
63	103545.0
64	103545.0
65	103545.0
66	83006.5
67	62468.0
68	62468.0
69	62468.0
70	46784.0
71	31100.0
72	31100.0
73	31100.0
74	22927.5
75	14755.0
76	14755.0
77	14755.0
78	10864.5
79	6974.0
80	6974.0
81	6974.0
82	4965.0
83	2956.0
84	2956.0
85	2956.0
86	2001.0
87	1046.0
88	1046.0
89	1046.0
90	676.5
91	307.0
92	307.0
93	307.0
94	200.5
95	94.0
96	94.0
97	94.0
98	332.0
99	570.0
100	570.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.566588879669531E-4
2	1.9133177759339065E-4
3	6.377725919779688E-4
4	0.010331915990043093
5	0.036097928705953033
6	0.06358592742020348
7	0.10172472842048602
8	0.1041482642700023
9	0.11467151203763878
10	0.12755451839559376
11	0.1345062396481536
12	0.12946783617152768
13	0.12360032832533034
14	0.11205664441052911
15	0.1287025090611541
16	0.11530928462961676
17	0.12149567877180305
18	0.10810245434026572
19	0.093880125539157
20	0.08979838095049801
21	0.08922438561771784
22	0.0998751879037499
23	0.07927513318286152
24	0.08909683109932225
25	0.08692840428659714
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1567957.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.668539800320524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.95262399920337	35.78965481638258
2	16.610947082697322	17.49748655092124
3	6.671950190768287	10.54205622504707
4	3.179616944588986	6.698631263834343
5	1.76313545255612	4.643088487815407
6	1.0586509820777716	3.345456083052692
7	0.6785627686650881	2.501723713191702
8	0.44444550059796345	1.8726636429853771
9	0.3252903752836745	1.5419312169560468
>10	1.2416493099383776	10.746737044725158
>50	0.04786460011540294	1.7271550054217015
>100	0.02380543441406592	2.27409920113669
>500	7.28679546816202E-4	0.26039803913384935
>1k	7.28679546816202E-4	0.5589187093961129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2230	0.14222328801108702	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2755451839559375E-4
2	0.0	0.0	0.0	0.0	1.2755451839559375E-4
3	0.0	0.0	0.0	0.0	1.2755451839559375E-4
4	0.0	0.0	0.0	0.0	1.2755451839559375E-4
5	0.0	0.0	0.0	0.0	1.2755451839559375E-4
6	0.0	0.0	0.0	0.0	2.551090367911875E-4
7	0.0	0.0	0.0	6.377725919779688E-5	2.551090367911875E-4
8	0.0	0.0	0.0	6.377725919779688E-5	2.551090367911875E-4
9	0.0	0.0	0.0	6.377725919779688E-5	2.551090367911875E-4
10	0.0	0.0	0.0	6.377725919779688E-5	2.551090367911875E-4
11	0.0	0.0	0.0	6.377725919779688E-5	3.8266355518678125E-4
12	0.0	0.0	0.0	6.377725919779688E-5	4.4644081438457815E-4
13	0.0	0.0	0.0	6.377725919779688E-5	5.739953327801719E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACT	45	6.7669374E-4	14.776462	4
GGTATCA	570	0.0	14.494542	1
GTATCAA	1465	0.0	12.316182	1
GAACCGC	55	0.0030653754	12.092535	6
TAGGACC	490	0.0	11.825477	4
AGGACGT	805	0.0	11.682935	5
GCACCGT	90	7.4493873E-6	11.612673	6
GGTTCTA	90	7.454686E-6	11.611931	13
TAGGACG	790	0.0	11.543276	4
TGTAGGA	1015	0.0	11.415515	2
TTAGGAC	550	0.0	11.398257	3
GGACGTG	820	0.0	11.355185	6
AGAACCG	85	5.3240525E-5	11.176189	5
GTAGGAC	935	0.0	11.174761	3
CCGAATA	60	0.0058726068	11.084115	14
AGTACCG	60	0.005876797	11.083054	5
TGGCGAG	720	0.0	11.082347	18
GACGTGA	515	0.0	11.06945	7
TACACCG	70	0.0014917264	10.85687	5
GTCCTAA	385	0.0	10.853057	1
>>END_MODULE