FastQCFastQC Report
Thu 2 Feb 2017
SRR4062029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567957
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14250.026.0971681
GGTATCA6200.025.9091221
GTCCTAC12250.021.1966611
GATTTCG4800.020.16868241
GTAGGAC12150.019.9165123
TCCTACA13550.019.1581442
ATTTCGT5000.018.92249542
GTATAGG2800.018.8614371
TAGGACG9050.018.4740414
TGTAGGA13900.018.3592072
CTGTAGG12500.018.3081681
TAGGACC6650.018.1944074
TATCAAC20550.017.8778532
TCAACGC20650.017.7907094
TTTCGTC5600.017.28744343
ATCAACG21150.017.2661133
CAACGCA21450.017.1271865
CCTACAG14650.016.6679523
AACGCAG22400.016.499026
TTCGTCA5750.016.4538244
Produced by FastQC (version 0.11.5)