##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062029_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1567957
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16468882756351	32.0	32.0	32.0	32.0	32.0
2	31.201931558072065	32.0	32.0	32.0	32.0	32.0
3	31.42956407605566	32.0	32.0	32.0	32.0	32.0
4	31.546173779000316	32.0	32.0	32.0	32.0	32.0
5	31.491845758525265	32.0	32.0	32.0	32.0	32.0
6	35.12151544972215	36.0	36.0	36.0	36.0	36.0
7	35.135191207411935	36.0	36.0	36.0	36.0	36.0
8	35.06281868699205	36.0	36.0	36.0	36.0	36.0
9	35.1916487505716	36.0	36.0	36.0	36.0	36.0
10	35.024531922750434	36.0	36.0	36.0	36.0	36.0
11	35.204533670247336	36.0	36.0	36.0	36.0	36.0
12	35.11314277113467	36.0	36.0	36.0	36.0	36.0
13	35.15565158993518	36.0	36.0	36.0	36.0	36.0
14	35.109681579278	36.0	36.0	36.0	36.0	36.0
15	35.068695123654535	36.0	36.0	36.0	36.0	36.0
16	35.09261733580704	36.0	36.0	36.0	36.0	36.0
17	35.07657735511879	36.0	36.0	36.0	36.0	36.0
18	35.071607831082105	36.0	36.0	36.0	36.0	36.0
19	35.05526490841267	36.0	36.0	36.0	36.0	36.0
20	35.065117219413544	36.0	36.0	36.0	36.0	36.0
21	35.05065827698081	36.0	36.0	36.0	36.0	36.0
22	35.022827156612074	36.0	36.0	36.0	36.0	36.0
23	34.9909366136954	36.0	36.0	36.0	36.0	36.0
24	34.97900197518172	36.0	36.0	36.0	36.0	36.0
25	34.95056752194097	36.0	36.0	36.0	32.0	36.0
26	34.90359365722402	36.0	36.0	36.0	32.0	36.0
27	34.88211156300842	36.0	36.0	36.0	32.0	36.0
28	34.85875951955315	36.0	36.0	36.0	32.0	36.0
29	34.83470783956448	36.0	36.0	36.0	32.0	36.0
30	34.795697841203555	36.0	36.0	36.0	32.0	36.0
31	34.793273667581445	36.0	36.0	36.0	32.0	36.0
32	34.767867996380005	36.0	36.0	36.0	32.0	36.0
33	34.76926982053717	36.0	36.0	36.0	32.0	36.0
34	34.74141829144549	36.0	36.0	36.0	32.0	36.0
35	34.734081355547374	36.0	36.0	36.0	32.0	36.0
36	34.69286785288117	36.0	36.0	36.0	32.0	36.0
37	34.67228119138471	36.0	36.0	36.0	32.0	36.0
38	34.65757287986852	36.0	36.0	36.0	32.0	36.0
39	34.606614211996884	36.0	36.0	36.0	32.0	36.0
40	34.615267510524845	36.0	36.0	36.0	32.0	36.0
41	34.58181506253042	36.0	36.0	36.0	32.0	36.0
42	34.57037852441106	36.0	36.0	36.0	32.0	36.0
43	34.55811160637696	36.0	36.0	36.0	32.0	36.0
44	34.51097510964905	36.0	36.0	36.0	32.0	36.0
45	34.48917795577302	36.0	36.0	36.0	32.0	36.0
46	34.450982393012055	36.0	36.0	36.0	32.0	36.0
47	34.42878408017567	36.0	36.0	36.0	32.0	36.0
48	34.407476097877684	36.0	36.0	36.0	32.0	36.0
49	34.38125599107629	36.0	36.0	36.0	32.0	36.0
50	33.935703593912336	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	14.0
21	21.0
22	97.0
23	256.0
24	674.0
25	1709.0
26	3741.0
27	7323.0
28	13190.0
29	21461.0
30	33425.0
31	51751.0
32	80832.0
33	142118.0
34	316385.0
35	894958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93722273470185	18.233706018722305	12.658990955266269	26.170080291309578
2	15.455862806176986	19.804526355266077	38.520770669258695	26.21884016929824
3	18.97898855515624	22.927443708524798	28.524032896352253	29.56953483996671
4	12.820449043212948	16.170606828252833	35.5810367127649	35.42790741576932
5	14.448100298668907	36.667395853330156	33.7078121402564	15.176691707744535
6	34.77282859160041	35.15695902374874	16.53604021028638	13.534172174364475
7	30.741085374152483	30.070339939169248	19.94416938729825	19.24440529938002
8	27.34596675801696	35.14828531649784	18.73297545787289	18.772772467612313
9	26.543648837308677	15.15577276672766	18.67933878288754	39.62123961307613
10	15.950437416332209	27.632454206333463	32.031171773205514	24.385936604128812
11	36.40399577284326	21.604418998735298	22.520643104370848	19.470942124050598
12	24.373117375030056	23.497391828985105	29.47319346130028	22.65629733468456
13	29.781237623225636	20.38671978887176	24.869495783366506	24.962546804536093
14	22.64443476447377	20.042769030018043	25.627743617969116	31.68505258753907
15	24.515914658373923	28.5028224626058	21.99078163495555	24.99048124406473
16	24.546094640893703	26.558214940801605	24.75177748241651	24.143912935888178
17	23.506448199791194	26.868530195662256	25.852494679382154	23.772526925164403
18	23.618231147087222	26.18271964611207	26.911695113504607	23.287354093296102
19	25.207706588892425	25.477930836113487	25.538519232351398	23.775843342642688
20	25.429078550186134	25.48692467184922	25.71582183904747	23.368174938917175
21	25.2344292604963	25.103558324622423	25.165613597821878	24.496398817059397
22	25.0608913382191	25.745795324744236	25.401908343149714	23.79140499388695
23	24.17062457707705	25.760336539841333	25.688395791466217	24.380643091615394
24	24.418744814065306	25.47847721505182	26.04067877467485	24.062099196208024
25	24.406136914730915	25.510123383721716	25.94470245938337	24.13903724216399
26	23.921747704333164	26.08494892069538	26.073022550406456	23.92028082456501
27	24.142463635662086	26.006743833193767	25.82440395139899	24.02638857974516
28	24.116767670067745	25.84777165365379	25.622634626202377	24.412826050076085
29	24.29932351116012	25.69739194323285	25.42416877079548	24.57911577481155
30	24.229274371818274	25.79496128035675	25.6895361933954	24.286228154429576
31	24.887401033448686	25.380272635549257	25.412607767829925	24.319718563172135
32	24.285561219023126	26.017383273892435	25.623874322042113	24.073181185042323
33	24.05156535404145	25.841671173607356	25.994737083788277	24.112026388562917
34	24.349818169758397	25.799931885409315	25.994900334577846	23.855349610254432
35	24.167163067283862	25.935199629580968	25.931883205757313	23.965754097377857
36	23.92973786908697	25.90141183718686	26.11053747009644	24.05831282362973
37	24.222063484977006	25.668301518562693	25.561855439975	24.547779556485303
38	24.2995976927924	26.0889336180352	25.53419866373801	24.077270025434387
39	24.762108850826305	25.632292990055767	25.418446066951372	24.187152092166556
40	25.10542387669042	25.46244920138065	25.575526354055857	23.85660056787308
41	24.606905102357103	25.497052824658056	25.909312972035952	23.986729100948885
42	24.406473730367537	26.056541497249462	25.793000079725743	23.743984692657257
43	24.06300705152409	25.763574949231067	26.15180421050483	24.021613788740012
44	23.982526991448456	25.835956793273056	25.737353817580665	24.44416239769782
45	24.13276714726153	25.931330592543745	25.586669711043562	24.349232549151168
46	24.329861007027777	25.35421108589017	25.737712550568553	24.578215356513496
47	24.58134585421263	25.630109806427665	25.625007573626814	24.163536765732893
48	24.278273980361092	25.856874255377228	25.398620327213077	24.466231437048602
49	24.45044116502774	25.59994489423392	25.51683978636311	24.43277415437523
50	24.20507365058956	26.226191751746235	25.32877449704836	24.239960100615836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	5.0
3	25.5
4	46.0
5	74.5
6	103.0
7	92.0
8	81.0
9	112.5
10	144.0
11	251.0
12	358.0
13	649.0
14	940.0
15	1295.0
16	1650.0
17	1735.5
18	1821.0
19	2064.0
20	2307.0
21	3025.0
22	3743.0
23	4862.5
24	5982.0
25	7860.0
26	9738.0
27	13264.5
28	16791.0
29	20869.0
30	24947.0
31	30014.0
32	35081.0
33	41417.0
34	47753.0
35	55064.0
36	62375.0
37	67403.5
38	72432.0
39	75547.0
40	78662.0
41	82454.0
42	86246.0
43	90183.0
44	94120.0
45	99370.5
46	104621.0
47	108322.5
48	112024.0
49	114579.0
50	117134.0
51	116294.5
52	115455.0
53	114085.0
54	112715.0
55	108758.0
56	104801.0
57	98901.5
58	93002.0
59	85839.0
60	78676.0
61	68606.0
62	58536.0
63	50342.5
64	42149.0
65	35082.0
66	28015.0
67	24037.5
68	20060.0
69	16655.5
70	13251.0
71	10631.5
72	8012.0
73	6737.0
74	5462.0
75	4421.5
76	3381.0
77	2775.0
78	2169.0
79	1829.5
80	1490.0
81	1172.5
82	855.0
83	649.0
84	443.0
85	324.0
86	205.0
87	145.0
88	85.0
89	58.0
90	31.0
91	22.0
92	13.0
93	13.0
94	13.0
95	15.0
96	17.0
97	13.5
98	10.0
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026467562567085705
2	0.004655739921439172
3	1.2755451839559375E-4
4	6.377725919779688E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.739953327801719E-4
17	0.0
18	1.9133177759339065E-4
19	0.0
20	2.551090367911875E-4
21	0.0
22	0.0
23	0.0
24	8.928816287691563E-4
25	1.9133177759339065E-4
26	1.9133177759339065E-4
27	3.826635551867813E-4
28	0.0014668769615493282
29	5.10218073582375E-4
30	0.0012117679247581406
31	1.9133177759339065E-4
32	8.291043695713594E-4
33	3.188862959889844E-4
34	7.015498511757657E-4
35	1.9133177759339065E-4
36	0.0
37	0.0017219859983405156
38	6.377725919779688E-5
39	8.291043695713594E-4
40	6.377725919779688E-5
41	9.566588879669531E-4
42	0.0052297352542193445
43	0.004400630884647985
44	0.0033801947374832346
45	0.002487313108714078
46	0.0022959813311206876
47	0.00102043614716475
48	0.0031250857006920466
49	0.004017967329461204
50	8.291043695713594E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1567957.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.062937949443125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.65464846930709	37.80326648606287
2	16.32862878837038	17.982045475470578
3	6.567973789404589	10.849557996586611
4	3.1444234591191065	6.9256477526499465
5	1.7384428895221522	4.786188647720444
6	1.0300452201770194	3.403038962623662
7	0.642944650365144	2.4781694971587633
8	0.4219992163168468	1.858921333021453
9	0.28746041894817614	1.4245573690317914
>10	1.138105199978323	10.07771874814986
>50	0.03296385953342797	1.2269269616191674
>100	0.012014155930664884	1.0736973912966055
>500	3.498830270805152E-4	0.11026337860817004
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.551090367911875E-4
2	0.0	0.0	0.0	0.0	2.551090367911875E-4
3	0.0	0.0	0.0	0.0	2.551090367911875E-4
4	0.0	0.0	0.0	0.0	2.551090367911875E-4
5	0.0	0.0	0.0	0.0	3.188862959889844E-4
6	0.0	0.0	0.0	0.0	4.4644081438457815E-4
7	0.0	0.0	0.0	6.377725919779688E-5	4.4644081438457815E-4
8	0.0	0.0	0.0	6.377725919779688E-5	4.4644081438457815E-4
9	0.0	0.0	0.0	6.377725919779688E-5	4.4644081438457815E-4
10	0.0	0.0	0.0	6.377725919779688E-5	4.4644081438457815E-4
11	0.0	0.0	0.0	6.377725919779688E-5	5.10218073582375E-4
12	0.0	0.0	0.0	6.377725919779688E-5	5.739953327801719E-4
13	0.0	0.0	0.0	6.377725919779688E-5	7.015498511757657E-4
14	0.0	0.0	0.0	6.377725919779688E-5	0.001084213406362547
15	0.0	0.0	0.0	6.377725919779688E-5	0.001084213406362547
16	0.0	0.0	0.0	3.188862959889844E-4	0.0011479906655603438
17	0.0	0.0	0.0	3.188862959889844E-4	0.0011479906655603438
18	0.0	0.0	0.0	3.188862959889844E-4	0.0011479906655603438
19	0.0	0.0	0.0	3.8266355518678125E-4	0.0011479906655603438
20	0.0	0.0	0.0	7.015498511757657E-4	0.0011479906655603438
21	0.0	0.0	0.0	8.928816287691563E-4	0.0012117679247581406
22	0.0	0.0	0.0	0.0011479906655603438	0.0012117679247581406
23	0.0	0.0	0.0	0.0014030997023515314	0.0012117679247581406
24	0.0	0.0	0.0	0.002487313108714078	0.0012117679247581406
25	0.0	0.0	0.0	0.003125085700692047	0.0012117679247581406
26	0.0	0.0	0.0	0.003762858292670016	0.0012117679247581406
27	0.0	0.0	0.0	0.004464408143845781	0.0012755451839559376
28	0.0	0.0	0.0	0.005484844291010532	0.0012755451839559376
29	0.0	0.0	0.0	0.007461939326142235	0.0013393224431537344
30	0.0	0.0	0.0	0.010459470508438687	0.0013393224431537344
31	0.0	0.0	0.0	0.01594431479944922	0.0013393224431537344
32	0.0	0.0	0.0	0.022768481533613487	0.0014030997023515314
33	0.0	0.0	0.0	0.03042175263734911	0.0014668769615493282
34	0.0	0.0	0.0	0.03915923714744728	0.0014668769615493282
35	0.0	0.0	0.0	0.04713139454717189	0.0014668769615493282
36	0.0	0.0	0.0	0.05663420616764363	0.0014668769615493282
37	0.0	0.0	0.0	0.07461939326142235	0.0014668769615493282
38	0.0	0.0	0.0	0.09942874708936533	0.0014668769615493282
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1425	0.0	26.097168	1
GGTATCA	620	0.0	25.909122	1
GTCCTAC	1225	0.0	21.196661	1
GATTTCG	480	0.0	20.168682	41
GTAGGAC	1215	0.0	19.916512	3
TCCTACA	1355	0.0	19.158144	2
ATTTCGT	500	0.0	18.922495	42
GTATAGG	280	0.0	18.861437	1
TAGGACG	905	0.0	18.474041	4
TGTAGGA	1390	0.0	18.359207	2
CTGTAGG	1250	0.0	18.308168	1
TAGGACC	665	0.0	18.194407	4
TATCAAC	2055	0.0	17.877853	2
TCAACGC	2065	0.0	17.790709	4
TTTCGTC	560	0.0	17.287443	43
ATCAACG	2115	0.0	17.266113	3
CAACGCA	2145	0.0	17.127186	5
CCTACAG	1465	0.0	16.667952	3
AACGCAG	2240	0.0	16.49902	6
TTCGTCA	575	0.0	16.45382	44
>>END_MODULE