FastQCFastQC Report
Thu 2 Feb 2017
SRR4062028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762428
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32620.18508557512704066No Hit
TCGTAGTTCCGACCATAAACGATGC30240.17158147737099047No Hit
GATTAAGAGGGACGGCCGGGGGCAT29300.16624792615641604No Hit
GCGCAAGACGGACCAGAGCGAAAGC28100.15943913737185292No Hit
ATCAGATACCGTCGTAGTTCCGACC27370.15529712419457703No Hit
GAATAGGACCGCGGTTCTATTTTGT27230.15450276550304465No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24920.14139584709276068No Hit
GGGTAGGCACACGCTGAGCCAGTCA24360.1382184123266312No Hit
GTATCTGATCGTCTTCGAACCTCCG23140.13129614372899204No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT22750.12908328737400904No Hit
GAATAACGCCGCCGCATCGCCAGTC22610.12828892868247668No Hit
ACCATACTCCCCCCGGAACCCAAAG22090.1253384535424993No Hit
GAACTACGACGGTATCTGATCGTCT22030.12499801410327117No Hit
GTACATGGGGAATAATTGCAATCCC21620.12267167793521211No Hit
TATCAACGCAGAGTACTTTTTTTTT20910.11864314457101226No Hit
GTATCAACGCAGAGTACATGGGGAA20530.11648702812256727No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC20470.11614658868333912No Hit
GTGCATGGCCGTTCTTAGTTGGTGG20160.11438765158066032No Hit
GTCCTAAAGTGTGTATTTCTCATTT20020.11359329288912795No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19780.11223153513221533No Hit
GTCCTACAGTGGACATTTCTAAATT19210.10899736045954785No Hit
CTGTAGGACGTGGAATATGGCAAGA18960.10757886279609721No Hit
GAATAATGGAATAGGACCGCGGTTC18470.10479860737573393No Hit
CTTTAGGACGTGAAATATGGCGAGG18460.1047418674691959No Hit
GAGTATGGTTGCAAAGCTGAAACTT18380.1042879482168917No Hit
GTTATTGCTCAATCTCGGGTGGCTG18370.10423120831035368No Hit
GCCATGCACCACCACCCACGGAATC18100.10269923083382697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.014.956861
ACTGTCG400.00527711214.2509598
TCGCGTA2350.013.7459979
CGCGTAA2550.012.66787910
CGCCAGT3750.012.666079518
CGCATCG3700.012.58335913
GTATCAA25600.012.4978211
CGAACGA1600.012.46958916
CCGACCA5450.012.3773329
CGGACAT3000.012.350485
GCATCGC3850.012.33989614
CGGACCA4950.012.2840059
CGGTCCA3500.012.21545510
CGCGCTA701.0903729E-412.21510816
AGCCCCG3600.012.13970616
CGACCAT5500.012.09206710
CGCAAGA4800.012.0685842
CATCGCC3950.012.02680915
GTCGCGT2800.011.8757998
GCGTAAC2650.011.83266611
Produced by FastQC (version 0.11.5)