##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062028_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762428
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.351110513450763	32.0	32.0	32.0	32.0	32.0
2	31.027596020943836	32.0	32.0	32.0	32.0	32.0
3	31.03605877800398	32.0	32.0	32.0	32.0	32.0
4	31.040171853828923	32.0	32.0	32.0	32.0	32.0
5	31.01185580347112	32.0	32.0	32.0	32.0	32.0
6	34.64689394403629	36.0	36.0	36.0	32.0	36.0
7	34.58094117887369	36.0	36.0	36.0	32.0	36.0
8	34.56126661628163	36.0	36.0	36.0	32.0	36.0
9	34.640592977415245	36.0	36.0	36.0	32.0	36.0
10	34.43180600852914	36.0	36.0	36.0	32.0	36.0
11	34.609153962601596	36.0	36.0	36.0	32.0	36.0
12	34.48699861781588	36.0	36.0	36.0	32.0	36.0
13	34.54860397133954	36.0	36.0	36.0	32.0	36.0
14	34.48987589847642	36.0	36.0	36.0	32.0	36.0
15	34.45195945593238	36.0	36.0	36.0	32.0	36.0
16	34.44306661038068	36.0	36.0	36.0	32.0	36.0
17	34.38910695926302	36.0	36.0	36.0	32.0	36.0
18	34.39261972687679	36.0	36.0	36.0	32.0	36.0
19	34.37846028320022	36.0	36.0	36.0	32.0	36.0
20	34.36766438118323	36.0	36.0	36.0	32.0	36.0
21	34.335793008281755	36.0	36.0	36.0	32.0	36.0
22	34.30868778752948	36.0	36.0	36.0	32.0	36.0
23	34.289524451495325	36.0	36.0	36.0	32.0	36.0
24	34.26757518605015	36.0	36.0	36.0	32.0	36.0
25	33.92139026388596	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	21.0
5	130.0
6	380.0
7	61.0
8	298.0
9	248.0
10	135.0
11	38.0
12	63.0
13	82.0
14	286.0
15	399.0
16	639.0
17	824.0
18	1029.0
19	1391.0
20	2009.0
21	2740.0
22	3808.0
23	5298.0
24	7792.0
25	10769.0
26	15599.0
27	20281.0
28	27917.0
29	37930.0
30	50924.0
31	71769.0
32	105369.0
33	157131.0
34	370151.0
35	866914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28951752375159	18.23618594559262	12.297379494419	25.176917036236784
2	15.85748710723816	20.15764660356793	38.772814471894655	25.21205181729925
3	18.671879211196003	24.373033593109454	28.636842060468165	28.318245135226377
4	12.136050144643646	16.28236890995777	36.73137138518563	34.85020956021295
5	14.24370441886878	37.42668848487738	33.4740959830994	14.85551111315444
6	33.173283825291115	36.46897514946092	17.332016146889	13.025724878358968
7	29.91184040050961	30.72527614840076	20.797963371819257	18.564920079270365
8	27.58313173214942	33.77175318902873	19.558940726127798	19.08617435269405
9	27.317399334091913	14.288335587680075	19.217714223062735	39.176550855165274
10	16.56811054042998	26.728955502022632	30.91370846779692	25.789225489750468
11	36.69086822179367	21.327577840633385	23.078715094020144	18.9028388435528
12	24.936025053634413	24.12940665793971	28.955083451510855	21.979484836915024
13	29.01491173798701	20.24023993314233	25.482897264278133	25.261951064592537
14	23.22865262907947	21.066208277923547	25.147227655110942	30.55791143788603
15	24.41019994454823	29.303932530646193	22.03952302384881	24.24634450095677
16	24.732263371493236	26.35299878657874	25.20462281686428	23.71011502506374
17	23.107626984320863	27.191792923339303	25.67216800919422	24.02841208314562
18	23.91036503034204	25.34893483349797	27.879567991665667	22.861132144494327
19	25.306113871690876	25.245399417618327	25.718336049107716	23.730150661583085
20	25.629067084965655	24.84134890065891	25.37321485567208	24.15636915870335
21	26.66196813555395	24.554103339667453	24.58278293127007	24.20114559350853
22	25.822620742771168	25.59378129219032	25.315697909206	23.26790005583251
23	23.410152694167024	25.66317105733144	26.31286943396569	24.61380681453584
24	24.382750054804823	25.55591397971597	26.347375104640292	23.713960860838917
25	24.003904930059385	25.576212865256633	26.079719408399686	24.340162796284297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	465.0
1	465.0
2	561.5
3	658.0
4	658.0
5	658.0
6	1382.5
7	2107.0
8	2107.0
9	2107.0
10	2542.5
11	2978.0
12	2978.0
13	2978.0
14	4296.0
15	5614.0
16	5614.0
17	5614.0
18	9580.5
19	13547.0
20	13547.0
21	13547.0
22	22205.5
23	30864.0
24	30864.0
25	30864.0
26	48007.0
27	65150.0
28	65150.0
29	65150.0
30	80895.0
31	96640.0
32	96640.0
33	96640.0
34	113293.5
35	129947.0
36	129947.0
37	129947.0
38	144075.5
39	158204.0
40	158204.0
41	158204.0
42	176635.0
43	195066.0
44	195066.0
45	195066.0
46	210485.0
47	225904.0
48	225904.0
49	225904.0
50	225687.5
51	225471.0
52	225471.0
53	225471.0
54	202406.0
55	179341.0
56	179341.0
57	179341.0
58	165770.5
59	152200.0
60	152200.0
61	152200.0
62	135112.5
63	118025.0
64	118025.0
65	118025.0
66	96855.0
67	75685.0
68	75685.0
69	75685.0
70	57615.5
71	39546.0
72	39546.0
73	39546.0
74	30564.0
75	21582.0
76	21582.0
77	21582.0
78	17738.0
79	13894.0
80	13894.0
81	13894.0
82	9557.0
83	5220.0
84	5220.0
85	5220.0
86	3887.0
87	2554.0
88	2554.0
89	2554.0
90	1748.5
91	943.0
92	943.0
93	943.0
94	558.5
95	174.0
96	174.0
97	174.0
98	411.5
99	649.0
100	649.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001134798130760519
2	2.8369953269012974E-4
3	7.376187849943373E-4
4	0.009418824485312307
5	0.03387372420320149
6	0.0623004173787525
7	0.10616036513264655
8	0.11001867877723233
9	0.12057230139330515
10	0.13322530055128493
11	0.14105540765353253
12	0.13413313905589336
13	0.12885632774785694
14	0.11682746756179543
15	0.13277138129898072
16	0.12006164223446292
17	0.12431713522481486
18	0.11342307316951387
19	0.09798981859117083
20	0.09339388616159071
21	0.09044341102161337
22	0.10241553130113684
23	0.08267004382590382
24	0.0927130072831344
25	0.08947883261046692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1762428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.876634794602694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37200297494506	33.201839498354545
2	15.781503912419817	14.480045829993548
3	5.553421459662975	7.6431686449640335
4	2.420089107633636	4.441021766452169
5	1.2119718521471121	2.7800595021145638
6	0.6631478038002622	1.8253793765872481
7	0.4353088311621223	1.3979352989063036
8	0.26845326370754874	0.9852585870824305
9	0.18949682733650186	0.7824129068207323
>10	0.8862970196684208	7.393473254629606
>50	0.09233034014938574	2.9678579921090313
>100	0.10038405799674284	10.073462374144574
>500	0.016274873628753708	5.088583774474779
>1k	0.009317675741652884	6.939501193366464
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3262	0.18508557512704066	No Hit
TCGTAGTTCCGACCATAAACGATGC	3024	0.17158147737099047	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2930	0.16624792615641604	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2810	0.15943913737185292	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2737	0.15529712419457703	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2723	0.15450276550304465	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2492	0.14139584709276068	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2436	0.1382184123266312	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2314	0.13129614372899204	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	2275	0.12908328737400904	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2261	0.12828892868247668	No Hit
ACCATACTCCCCCCGGAACCCAAAG	2209	0.1253384535424993	No Hit
GAACTACGACGGTATCTGATCGTCT	2203	0.12499801410327117	No Hit
GTACATGGGGAATAATTGCAATCCC	2162	0.12267167793521211	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2091	0.11864314457101226	No Hit
GTATCAACGCAGAGTACATGGGGAA	2053	0.11648702812256727	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2047	0.11614658868333912	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2016	0.11438765158066032	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2002	0.11359329288912795	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1978	0.11223153513221533	No Hit
GTCCTACAGTGGACATTTCTAAATT	1921	0.10899736045954785	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1896	0.10757886279609721	No Hit
GAATAATGGAATAGGACCGCGGTTC	1847	0.10479860737573393	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1846	0.1047418674691959	No Hit
GAGTATGGTTGCAAAGCTGAAACTT	1838	0.1042879482168917	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	1837	0.10423120831035368	No Hit
GCCATGCACCACCACCCACGGAATC	1810	0.10269923083382697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.673990653802595E-5
2	0.0	0.0	0.0	0.0	5.673990653802595E-5
3	0.0	0.0	0.0	0.0	5.673990653802595E-5
4	0.0	0.0	0.0	0.0	5.673990653802595E-5
5	0.0	0.0	0.0	0.0	5.673990653802595E-5
6	0.0	0.0	0.0	0.0	5.673990653802595E-5
7	0.0	0.0	0.0	0.0	5.673990653802595E-5
8	0.0	0.0	0.0	0.0	5.673990653802595E-5
9	0.0	0.0	0.0	0.0	5.673990653802595E-5
10	0.0	0.0	0.0	0.0	5.673990653802595E-5
11	0.0	0.0	0.0	0.0	5.673990653802595E-5
12	0.0	0.0	0.0	0.0	1.7021971961407784E-4
13	0.0	0.0	0.0	0.0	1.7021971961407784E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	895	0.0	14.95686	1
ACTGTCG	40	0.005277112	14.250959	8
TCGCGTA	235	0.0	13.745997	9
CGCGTAA	255	0.0	12.667879	10
CGCCAGT	375	0.0	12.6660795	18
CGCATCG	370	0.0	12.583359	13
GTATCAA	2560	0.0	12.497821	1
CGAACGA	160	0.0	12.469589	16
CCGACCA	545	0.0	12.377332	9
CGGACAT	300	0.0	12.35048	5
GCATCGC	385	0.0	12.339896	14
CGGACCA	495	0.0	12.284005	9
CGGTCCA	350	0.0	12.215455	10
CGCGCTA	70	1.0903729E-4	12.215108	16
AGCCCCG	360	0.0	12.139706	16
CGACCAT	550	0.0	12.092067	10
CGCAAGA	480	0.0	12.068584	2
CATCGCC	395	0.0	12.026809	15
GTCGCGT	280	0.0	11.875799	8
GCGTAAC	265	0.0	11.832666	11
>>END_MODULE