FastQCFastQC Report
Thu 2 Feb 2017
SRR4062028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762428
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA32370.18366707746359No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT29560.1677231637264047No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT28590.1622193927922162No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG28080.15932565755877687No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27640.15682910167110373No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA26430.14996357298000257No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC23160.13140962354206812No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA21990.12477105447711906No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC21080.11960772298215872No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20060.11382025251528007No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC20060.11382025251528007No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20030.11365003279566598No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19430.11024563840338442No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG19340.10973497924454219No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG18720.10621710503918458No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG18700.10610362522610853No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT18640.10576318578688036No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT18240.10349358952535934No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT17900.10156443270306645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.027.4174521
GTATCAA31700.026.7238771
TGTAGGA16600.020.9398272
GTAGGAC16950.020.5062773
TTAGGAC9300.019.8699043
GTCCTAC14200.019.679471
CTGTAGG16700.019.6321471
ATTGCGA901.002604E-519.55506317
TAGGACG14700.019.4547424
GACGTGA8500.019.1519177
GGACGTG15000.019.0656456
GTCGTAA708.1251626E-418.85720330
TACCGTC7800.018.6143887
AGGACGT15700.018.3557055
ATACCGT7800.018.3323526
GATATAC4100.018.2470661
AACGCAG47250.018.0646426
TCAACGC47150.018.0562974
ATCAACG47250.018.0180843
TAGGACA4900.017.9582214
Produced by FastQC (version 0.11.5)