##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762428
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30240497767852	32.0	32.0	32.0	32.0	32.0
2	31.477477661498796	32.0	32.0	32.0	32.0	32.0
3	31.59052000989544	32.0	32.0	32.0	32.0	32.0
4	31.660242574448432	32.0	32.0	32.0	32.0	32.0
5	31.635439291704397	32.0	32.0	32.0	32.0	32.0
6	35.28873066020286	36.0	36.0	36.0	36.0	36.0
7	35.29129587137744	36.0	36.0	36.0	36.0	36.0
8	35.23959617073719	36.0	36.0	36.0	36.0	36.0
9	35.32454602400779	36.0	36.0	36.0	36.0	36.0
10	35.20702235779277	36.0	36.0	36.0	36.0	36.0
11	35.32691775210108	36.0	36.0	36.0	36.0	36.0
12	35.237595521632656	36.0	36.0	36.0	36.0	36.0
13	35.280649195314645	36.0	36.0	36.0	36.0	36.0
14	35.24767990522166	36.0	36.0	36.0	36.0	36.0
15	35.219132356045186	36.0	36.0	36.0	36.0	36.0
16	35.22596100379703	36.0	36.0	36.0	36.0	36.0
17	35.21063952683457	36.0	36.0	36.0	36.0	36.0
18	35.210772865614935	36.0	36.0	36.0	36.0	36.0
19	35.19448510804413	36.0	36.0	36.0	36.0	36.0
20	35.19362266146475	36.0	36.0	36.0	36.0	36.0
21	35.17782286709017	36.0	36.0	36.0	36.0	36.0
22	35.15951914064007	36.0	36.0	36.0	36.0	36.0
23	35.13317650423166	36.0	36.0	36.0	36.0	36.0
24	35.11363187602557	36.0	36.0	36.0	36.0	36.0
25	35.08669006620412	36.0	36.0	36.0	36.0	36.0
26	35.048001393532104	36.0	36.0	36.0	36.0	36.0
27	35.02980944469788	36.0	36.0	36.0	36.0	36.0
28	34.997784874048754	36.0	36.0	36.0	36.0	36.0
29	34.972872083285104	36.0	36.0	36.0	36.0	36.0
30	34.94111475759577	36.0	36.0	36.0	36.0	36.0
31	34.946857970935554	36.0	36.0	36.0	36.0	36.0
32	34.915662370320945	36.0	36.0	36.0	32.0	36.0
33	34.91351249526222	36.0	36.0	36.0	32.0	36.0
34	34.89680599718116	36.0	36.0	36.0	32.0	36.0
35	34.888048760006086	36.0	36.0	36.0	32.0	36.0
36	34.84338026858402	36.0	36.0	36.0	32.0	36.0
37	34.81436404777954	36.0	36.0	36.0	32.0	36.0
38	34.79728420111346	36.0	36.0	36.0	32.0	36.0
39	34.741336951069776	36.0	36.0	36.0	32.0	36.0
40	34.74995914726729	36.0	36.0	36.0	32.0	36.0
41	34.72726829124367	36.0	36.0	36.0	32.0	36.0
42	34.68591341036343	36.0	36.0	36.0	32.0	36.0
43	34.70467389306116	36.0	36.0	36.0	32.0	36.0
44	34.618569950091576	36.0	36.0	36.0	32.0	36.0
45	34.61770636871407	36.0	36.0	36.0	32.0	36.0
46	34.555289634526915	36.0	36.0	36.0	32.0	36.0
47	34.55145344944588	36.0	36.0	36.0	32.0	36.0
48	34.51550588165871	36.0	36.0	36.0	32.0	36.0
49	34.49771678616091	36.0	36.0	36.0	32.0	36.0
50	34.091779068421516	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	19.0
21	40.0
22	141.0
23	380.0
24	799.0
25	1927.0
26	3960.0
27	7878.0
28	13831.0
29	22196.0
30	33367.0
31	49602.0
32	75870.0
33	130050.0
34	299448.0
35	1122914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98971161066333	18.87860131937937	12.664816001462068	25.466871068495234
2	15.390839601962611	20.668460864971543	38.44918168782879	25.49151784523706
3	18.09034277281081	24.78023128412564	28.99346977821985	28.1359561648437
4	11.840149975006058	16.7455446381609	36.95846693224741	34.45583845458564
5	13.925845481347324	37.84097846834027	33.24652127633016	14.986654773982256
6	33.30040523687236	36.6833557834485	17.101200277344976	12.915038702334169
7	29.86771658189725	30.87853801687218	20.751372538339155	18.50237286289142
8	27.53735188047398	33.97330273917573	19.41208378441559	19.0772615959347
9	27.437546384873595	14.080688686289596	19.08787195845731	39.393892970379504
10	16.502064197799854	26.779306729125956	30.916780713878804	25.801848359195382
11	36.78408422925646	21.36546854680021	23.071410576772497	18.779036647170834
12	24.891002639540453	24.140844335201212	29.033639955788264	21.93451306947007
13	29.092933158120506	20.149872789129542	25.512872015197217	25.24432203755274
14	23.310228843391048	21.020035995796707	25.164262029427586	30.505473131384658
15	24.467893156486394	29.226555638017555	21.99011817787734	24.315433027618717
16	24.875724361996877	26.313128648052412	25.110345872392	23.70080111755871
17	23.225516162929775	27.170244685172957	25.523709337345977	24.08052981455129
18	24.05492432302084	25.306608791863482	27.82223357022285	22.816233314892834
19	25.45267097436037	25.13912625083124	25.726497763312885	23.681705011495506
20	25.85111519765686	24.716257107402704	25.31066605463047	24.121961640309962
21	26.79865503725542	24.591926592178517	24.464829201533338	24.14458916903272
22	25.975585953014818	25.521553220897538	25.252265624468063	23.250595201619582
23	23.42555091674771	25.54036311311794	26.354978875708824	24.679107094425525
24	24.542032052791498	25.45385425030002	26.29900618756756	23.705107509340927
25	24.24318765337532	25.358979814743886	26.079691334383025	24.318141197497766
26	23.321045312101933	26.86060822016987	26.66842939343097	23.14991707429723
27	24.530304921641832	25.697563577353865	25.613871835317347	24.158259665686955
28	23.65248939229391	25.760242578577596	26.18239466908156	24.404873360046935
29	23.97853403676086	25.375705422890597	26.01732392655999	24.62843661378856
30	23.53448838377104	25.700959768044235	26.687679619610705	24.07687222857402
31	25.011645951719657	25.149922123903846	25.390047179580293	24.448384744796204
32	24.431405525142758	25.678216720683427	25.155298181587092	24.73507957258672
33	23.42231315768868	25.60692047648066	26.433964169761836	24.53680219606882
34	24.59505848509006	25.665124275496975	26.49086622617261	23.248951013240355
35	25.036795871145795	25.23907386749855	26.497055762908627	23.227074498447024
36	23.137381732555994	26.032143211767877	26.204803041264167	24.62567201441196
37	24.815889740949107	25.786841201887206	25.233333049628488	24.1639360075352
38	23.856123484193397	25.670438735653317	25.85966632395763	24.613771456195657
39	25.395437510461498	24.944238445542055	25.250239018619336	24.41008502537711
40	25.514361729592206	25.172163159098222	26.075292763898872	23.238182347410703
41	24.13041776034698	25.77556261412008	26.52754939576645	23.56647022976649
42	25.428931825416505	26.376760937002615	25.68535182992187	22.50895540765901
43	24.401849802820095	25.310920078304537	26.209095809572446	24.07813430930292
44	23.702823564258342	26.092818270181752	25.54207542555467	24.66228274000523
45	24.06568795949598	26.44813046806246	25.491590127741087	23.994591444700472
46	23.903346885044982	25.681845907676244	25.72105413970273	24.69375306757604
47	24.60007773468641	25.140759360078984	26.06409990893126	24.195062996303346
48	24.873918490744188	26.192610783770782	24.740573960253116	24.192896765231918
49	24.23802760718965	26.27018095549905	25.087964084665682	24.403827352645617
50	24.296966007091477	26.765081874288256	24.84077083653683	24.097181282083437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	213.0
1	133.5
2	54.0
3	147.0
4	240.0
5	253.0
6	266.0
7	220.0
8	174.0
9	246.5
10	319.0
11	563.5
12	808.0
13	1436.5
14	2065.0
15	2736.5
16	3408.0
17	3467.5
18	3527.0
19	3597.5
20	3668.0
21	4443.5
22	5219.0
23	6420.0
24	7621.0
25	10165.5
26	12710.0
27	16979.0
28	21248.0
29	25871.0
30	30494.0
31	36157.0
32	41820.0
33	49333.0
34	56846.0
35	65278.5
36	73711.0
37	80983.5
38	88256.0
39	89642.5
40	91029.0
41	94838.5
42	98648.0
43	97017.0
44	95386.0
45	102860.5
46	110335.0
47	116224.5
48	122114.0
49	127467.0
50	132820.0
51	122941.5
52	113063.0
53	112722.5
54	112382.0
55	114126.0
56	115870.0
57	112552.0
58	109234.0
59	98892.5
60	88551.0
61	76904.0
62	65257.0
63	55966.0
64	46675.0
65	39446.5
66	32218.0
67	28495.5
68	24773.0
69	22487.5
70	20202.0
71	15528.0
72	10854.0
73	9509.0
74	8164.0
75	5776.5
76	3389.0
77	3361.0
78	3333.0
79	2797.5
80	2262.0
81	1760.5
82	1259.0
83	1137.5
84	1016.0
85	779.0
86	542.0
87	380.5
88	219.0
89	146.5
90	74.0
91	52.0
92	30.0
93	27.0
94	24.0
95	19.0
96	14.0
97	14.0
98	14.0
99	12.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030582809623995986
2	0.004595932429580102
3	3.9717934576618164E-4
4	5.673990653802595E-5
5	0.0
6	1.134798130760519E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.510985980703892E-4
17	0.0
18	1.7021971961407784E-4
19	0.0
20	2.8369953269012974E-4
21	0.0
22	0.0
23	1.134798130760519E-4
24	0.0013050178503745968
25	1.7021971961407784E-4
26	3.9717934576618164E-4
27	4.539192523042076E-4
28	0.0017021971961407784
29	5.673990653802595E-4
30	0.0013050178503745968
31	7.376187849943373E-4
32	6.808788784563114E-4
33	3.404394392281557E-4
34	7.376187849943373E-4
35	1.134798130760519E-4
36	1.7021971961407784E-4
37	0.0018724169157548563
38	0.0
39	7.376187849943373E-4
40	5.673990653802595E-5
41	0.0017021971961407784
42	0.004822892055732206
43	0.004425712709966024
44	0.004595932429580102
45	0.0019858967288309084
46	0.002439815981135116
47	0.0013050178503745968
48	0.004312232896889972
49	0.0034611342988195835
50	0.0017589371026788046
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1762428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.07288004739921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49885402873703	35.57727567326117
2	16.00363315345405	15.706887401240632
3	5.5568133054008735	8.18066498345187
4	2.38015238476374	4.672037298881686
5	1.1507106269619367	2.8234342283085327
6	0.6389230674653118	1.881227702954496
7	0.37929019047124135	1.3029003414105296
8	0.2510049663508358	0.9854029284028832
9	0.1804728484207795	0.7970690198128836
>10	0.7828264517563938	6.815544207008715
>50	0.07531604566921896	2.613673799970797
>100	0.08164937236317157	8.593251321006894
>500	0.013491501425742732	4.655071794255938
>1k	0.006862056759645009	5.395559300032921
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3237	0.18366707746359	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2956	0.1677231637264047	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2859	0.1622193927922162	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2808	0.15932565755877687	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2764	0.15682910167110373	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2643	0.14996357298000257	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2316	0.13140962354206812	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2199	0.12477105447711906	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2108	0.11960772298215872	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	2006	0.11382025251528007	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	2006	0.11382025251528007	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2003	0.11365003279566598	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1943	0.11024563840338442	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1934	0.10973497924454219	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1872	0.10621710503918458	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1870	0.10610362522610853	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1864	0.10576318578688036	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	1824	0.10349358952535934	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT	1790	0.10156443270306645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.7021971961407784E-4
7	0.0	0.0	0.0	0.0	1.7021971961407784E-4
8	0.0	0.0	0.0	0.0	1.7021971961407784E-4
9	0.0	0.0	0.0	0.0	1.7021971961407784E-4
10	0.0	0.0	0.0	0.0	1.7021971961407784E-4
11	0.0	0.0	0.0	0.0	1.7021971961407784E-4
12	0.0	0.0	0.0	0.0	1.7021971961407784E-4
13	0.0	0.0	0.0	0.0	1.7021971961407784E-4
14	0.0	0.0	0.0	0.0	1.7021971961407784E-4
15	0.0	0.0	0.0	5.673990653802595E-5	1.7021971961407784E-4
16	0.0	0.0	0.0	1.7021971961407784E-4	1.7021971961407784E-4
17	0.0	0.0	0.0	1.7021971961407784E-4	2.269596261521038E-4
18	0.0	0.0	0.0	2.269596261521038E-4	2.269596261521038E-4
19	0.0	0.0	0.0	2.269596261521038E-4	2.269596261521038E-4
20	0.0	0.0	0.0	2.8369953269012974E-4	2.269596261521038E-4
21	0.0	0.0	0.0	3.404394392281557E-4	2.269596261521038E-4
22	0.0	0.0	0.0	4.539192523042076E-4	2.269596261521038E-4
23	0.0	0.0	0.0	7.376187849943373E-4	2.269596261521038E-4
24	0.0	0.0	0.0	9.078385046084152E-4	2.269596261521038E-4
25	0.0	0.0	0.0	0.001078058224222493	2.269596261521038E-4
26	0.0	0.0	0.0	0.0012482779438365709	2.269596261521038E-4
27	0.0	0.0	0.0	0.0015319774765267006	2.269596261521038E-4
28	0.0	0.0	0.0	0.002269596261521038	2.269596261521038E-4
29	0.0	0.0	0.0	0.003177434766129453	2.269596261521038E-4
30	0.0	0.0	0.0	0.004595932429580102	2.269596261521038E-4
31	0.0	0.0	0.0	0.008624465793779944	2.269596261521038E-4
32	0.0	0.0	0.0	0.012823218877593865	2.269596261521038E-4
33	0.0	0.0	0.0	0.017475891213711993	2.269596261521038E-4
34	0.0	0.0	0.0	0.02201508373675407	2.269596261521038E-4
35	0.0	0.0	0.0	0.026667756072872195	2.269596261521038E-4
36	0.0	0.0	0.0	0.034497863175119776	2.269596261521038E-4
37	0.0	0.0	0.0	0.04624302382849115	2.269596261521038E-4
38	0.0	0.0	0.0	0.06105213943491592	2.269596261521038E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1260	0.0	27.417452	1
GTATCAA	3170	0.0	26.723877	1
TGTAGGA	1660	0.0	20.939827	2
GTAGGAC	1695	0.0	20.506277	3
TTAGGAC	930	0.0	19.869904	3
GTCCTAC	1420	0.0	19.67947	1
CTGTAGG	1670	0.0	19.632147	1
ATTGCGA	90	1.002604E-5	19.555063	17
TAGGACG	1470	0.0	19.454742	4
GACGTGA	850	0.0	19.151917	7
GGACGTG	1500	0.0	19.065645	6
GTCGTAA	70	8.1251626E-4	18.857203	30
TACCGTC	780	0.0	18.614388	7
AGGACGT	1570	0.0	18.355705	5
ATACCGT	780	0.0	18.332352	6
GATATAC	410	0.0	18.247066	1
AACGCAG	4725	0.0	18.064642	6
TCAACGC	4715	0.0	18.056297	4
ATCAACG	4725	0.0	18.018084	3
TAGGACA	490	0.0	17.958221	4
>>END_MODULE