FastQCFastQC Report
Thu 2 Feb 2017
SRR4062027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108869
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16820.15168608735567501No Hit
GCGCAAGACGGACCAGAGCGAAAGC12350.11137474309408955No Hit
TATCAACGCAGAGTACTTTTTTTTT11940.10767728198732222No Hit
GATTAAGAGGGACGGCCGGGGGCAT11290.10181545340342277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA1350.016.18630210
CGACCAT2400.014.25098210
TCGGGGG954.9058144E-913.9978042
GATAACG551.9582044E-413.81913511
CCGACCA2350.013.746259
CGAACGA655.45313E-513.1529716
CTTATAT655.4554417E-513.1523763
GGTCCAA1900.013.00089611
GGTATCA5100.012.8497641
ATTCCGA604.0833227E-412.6692576
AATTCCG604.0848795E-412.6686845
CGCCGGT1650.012.6686847
CGTCGTA1950.012.66754110
ACGAACG604.091112E-412.66639615
GTATTAG1352.7284841E-1112.6635351
TCTATAC1001.4430589E-712.348623
GTATCAA11700.012.338831
GACCATA2650.012.18952111
GCCGGTC1950.012.1814278
GTCGCGT1103.8005965E-812.0928358
Produced by FastQC (version 0.11.5)