FastQCFastQC Report
Thu 2 Feb 2017
SRR4062027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108869
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12210.11011219539909584No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11450.10325836505484416No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11230.10127436153413974No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11230.10127436153413974No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11160.10064308768664289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.025.8523711
CGGATCG901.5128535E-824.44366826
GGTATCA7250.023.3732071
GTCTACG500.002575378922.007241
ATCGATT1057.747258E-820.95171529
GATTTCG901.0001624E-519.55934541
GCGTAGA851.4270956E-418.123611
TATGCGA851.4320339E-418.11543812
ATACCGT2700.017.923746
CGTCGTA2600.017.76706310
TACCGTC2500.017.5978537
CTTACAC2500.017.5978533
CCGTCGT2600.016.9210139
GGTATAT1053.7632555E-516.7674221
GTCCTAG1854.2018655E-1016.6541271
ACCGTCG2650.016.601758
CGAATGC2800.016.50319743
GGATCGA1206.40751E-616.49947527
GACGGAC4950.016.4424387
TCAACGC20900.016.3137994
Produced by FastQC (version 0.11.5)