##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1108869
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9301531560536	32.0	32.0	32.0	32.0	32.0
2	31.31098804277151	32.0	32.0	32.0	32.0	32.0
3	31.482442019751655	32.0	32.0	32.0	32.0	32.0
4	31.59636981464898	32.0	32.0	32.0	32.0	32.0
5	31.520072253800944	32.0	32.0	32.0	32.0	32.0
6	35.2021943078939	36.0	36.0	36.0	36.0	36.0
7	35.19675813824717	36.0	36.0	36.0	36.0	36.0
8	35.1369115738649	36.0	36.0	36.0	36.0	36.0
9	35.26129596913612	36.0	36.0	36.0	36.0	36.0
10	35.100702607792265	36.0	36.0	36.0	36.0	36.0
11	35.27468528744153	36.0	36.0	36.0	36.0	36.0
12	35.17102921986276	36.0	36.0	36.0	36.0	36.0
13	35.23042577617374	36.0	36.0	36.0	36.0	36.0
14	35.18252561844546	36.0	36.0	36.0	36.0	36.0
15	35.15400286237599	36.0	36.0	36.0	36.0	36.0
16	35.15828470270158	36.0	36.0	36.0	36.0	36.0
17	35.14847109983235	36.0	36.0	36.0	36.0	36.0
18	35.14500360277003	36.0	36.0	36.0	36.0	36.0
19	35.13912013051136	36.0	36.0	36.0	36.0	36.0
20	35.137465291211136	36.0	36.0	36.0	36.0	36.0
21	35.12820901296727	36.0	36.0	36.0	36.0	36.0
22	35.11653585770727	36.0	36.0	36.0	36.0	36.0
23	35.08304768191734	36.0	36.0	36.0	36.0	36.0
24	35.0618440951997	36.0	36.0	36.0	36.0	36.0
25	35.04143861898926	36.0	36.0	36.0	36.0	36.0
26	35.002204047547544	36.0	36.0	36.0	36.0	36.0
27	34.992406677434396	36.0	36.0	36.0	36.0	36.0
28	34.960769035837416	36.0	36.0	36.0	36.0	36.0
29	34.94530463021331	36.0	36.0	36.0	36.0	36.0
30	34.92239209500852	36.0	36.0	36.0	32.0	36.0
31	34.92819620712636	36.0	36.0	36.0	32.0	36.0
32	34.897601069197535	36.0	36.0	36.0	32.0	36.0
33	34.88740689838024	36.0	36.0	36.0	32.0	36.0
34	34.877409324275455	36.0	36.0	36.0	32.0	36.0
35	34.856100224643306	36.0	36.0	36.0	32.0	36.0
36	34.82713106778168	36.0	36.0	36.0	32.0	36.0
37	34.82560699234986	36.0	36.0	36.0	32.0	36.0
38	34.79906733798131	36.0	36.0	36.0	32.0	36.0
39	34.78110849883981	36.0	36.0	36.0	32.0	36.0
40	34.77042644352038	36.0	36.0	36.0	32.0	36.0
41	34.74981174512048	36.0	36.0	36.0	32.0	36.0
42	34.70990441612129	36.0	36.0	36.0	32.0	36.0
43	34.71593849228358	36.0	36.0	36.0	32.0	36.0
44	34.66023488798046	36.0	36.0	36.0	32.0	36.0
45	34.64892516609266	36.0	36.0	36.0	32.0	36.0
46	34.62827980582016	36.0	36.0	36.0	32.0	36.0
47	34.60061828764263	36.0	36.0	36.0	32.0	36.0
48	34.57180334196375	36.0	36.0	36.0	32.0	36.0
49	34.5600517283827	36.0	36.0	36.0	32.0	36.0
50	34.121604986702664	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	30.0
22	67.0
23	178.0
24	476.0
25	1169.0
26	2488.0
27	4842.0
28	8185.0
29	13462.0
30	20850.0
31	32299.0
32	50648.0
33	90568.0
34	206579.0
35	677015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.214502862359566	18.4882447502359	12.353431493713273	25.94382089369126
2	16.203668213952994	19.66535686940116	37.21189044599544	26.91908447065041
3	18.687453048922908	22.83186351474072	28.535252649776755	29.945430786559612
4	12.816933289685256	15.97222034343101	35.06040839810654	36.1504379687772
5	14.97399602658204	36.33801648346198	32.873675790377405	15.814311699578578
6	35.449607572414	34.66376039687356	16.197073228800207	13.689558801912222
7	31.38432042017587	29.91309162759532	19.623778823287513	19.07880912894129
8	28.31434551782041	33.37589922705027	18.61653630861716	19.693218946512168
9	26.953589648551812	14.588287705761458	18.704283373419223	39.75383927226751
10	16.939452612441347	26.31190214876987	30.34051874571071	26.408126493078072
11	37.42678350643764	21.12891603967646	21.765330259931513	19.67897019395438
12	24.64520155221221	23.546785057567664	28.161937974639024	23.646075415581098
13	29.448474075837634	19.13968196423563	25.159419191987514	26.252424767939225
14	23.607838256818432	19.568767816577072	24.609399306861317	32.21399461974318
15	25.393802153365275	27.02564504914467	21.67641082941267	25.904141968077383
16	26.044088443827995	25.76957276919941	23.327158834913632	24.85917995205896
17	24.215484426023274	26.16891625611321	24.767307950713747	24.848291367149773
18	25.163725824400785	25.044234379988207	25.70527006870082	24.086769726910195
19	25.845974592129455	25.009536744196115	24.991229802618705	24.153258861055722
20	25.848075147177653	24.563787804455732	24.590481790372852	24.997655257993767
21	26.759518031435636	24.192307657622315	24.337230096611954	24.710944214330098
22	25.97385263723668	24.28970419409326	24.827278966225947	24.90916420244411
23	24.584621433750456	24.36430666228983	25.39508760285264	25.655984301107075
24	24.98780275544998	24.7630668336855	25.13435005595852	25.114780354905996
25	25.052643729782325	24.365276691836456	25.28269795620583	25.29938162217539
26	24.696153273843073	25.22236701492066	25.537644348049582	24.54383536318668
27	25.301389530185848	24.78238990534457	24.841188820270112	25.075031744199467
28	24.847228284591882	24.560352944147237	25.45470778576608	25.137710985494806
29	24.790167946806775	24.921563800036616	25.290590451660844	24.997677801495765
30	24.749221488627377	24.87764317349463	25.543199377371266	24.829935960506724
31	25.28522510936898	24.722124774881614	24.624547170474926	25.368102945274472
32	25.26901502443951	24.828201937124614	24.450426564219107	25.452356474216764
33	24.778827302225164	24.571768173136064	25.049916040048263	25.59948848459051
34	25.378969268521	24.652188773545515	25.367425912849466	24.60141604508402
35	25.817229823567818	24.542596594003978	25.24349156076287	24.396682021665338
36	24.58831889819167	25.043918662996855	25.048067037735784	25.31969540107569
37	25.684896298340448	24.855481424966676	24.740768396921506	24.71885387977137
38	25.021440765320342	24.610120762687025	25.25113426383098	25.11730420816165
39	25.590828828731333	24.517570202350868	24.604145135661117	25.28745583325668
40	25.870797966935648	24.650183791037346	24.970420284470286	24.508597957556717
41	24.75199754703028	25.156106271305667	25.36677308226467	24.725123099399383
42	25.876624577143232	25.091470535980815	24.90641010725749	24.12549477961846
43	25.293597756193464	24.272882227212467	25.161386326127538	25.272133690466525
44	24.77349917841032	25.05280359587528	24.998602125130542	25.17509510058386
45	25.048338455766704	25.01704471667893	24.939847481350014	24.99476934620435
46	25.04975898344674	24.82871817070916	24.9229603776903	25.198562468153803
47	25.220135995526938	24.76299983767112	25.25530725249355	24.76155691430839
48	25.868344585393306	25.183120286715592	24.1670980507452	24.781437077145902
49	25.108244238322204	25.3671646367584	24.5552313915055	24.9693597334139
50	25.17080666921584	25.38977107938272	24.453220255831233	24.98620199557021
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	67.0
2	30.0
3	78.5
4	127.0
5	133.0
6	139.0
7	121.5
8	104.0
9	124.5
10	145.0
11	258.0
12	371.0
13	742.5
14	1114.0
15	1380.5
16	1647.0
17	1763.0
18	1879.0
19	1915.0
20	1951.0
21	2364.5
22	2778.0
23	3417.0
24	4056.0
25	5294.5
26	6533.0
27	8367.5
28	10202.0
29	11880.0
30	13558.0
31	15446.5
32	17335.0
33	19783.0
34	22231.0
35	25839.0
36	29447.0
37	35114.5
38	40782.0
39	44074.0
40	47366.0
41	51455.0
42	55544.0
43	57427.5
44	59311.0
45	65080.5
46	70850.0
47	75415.5
48	79981.0
49	83200.5
50	86420.0
51	84836.0
52	83252.0
53	82759.5
54	82267.0
55	83396.5
56	84526.0
57	81415.0
58	78304.0
59	71653.0
60	65002.0
61	56863.0
62	48724.0
63	41864.5
64	35005.0
65	29520.5
66	24036.0
67	20682.0
68	17328.0
69	15562.5
70	13797.0
71	10897.5
72	7998.0
73	7009.0
74	6020.0
75	4403.5
76	2787.0
77	2432.5
78	2078.0
79	1809.0
80	1540.0
81	1260.0
82	980.0
83	801.0
84	622.0
85	487.0
86	352.0
87	239.0
88	126.0
89	88.0
90	50.0
91	37.5
92	25.0
93	19.5
94	14.0
95	14.5
96	15.0
97	14.0
98	13.0
99	8.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030932418527346333
2	0.004148370997836535
3	1.803639564276754E-4
4	0.0
5	0.0
6	1.803639564276754E-4
7	0.0
8	0.0
9	0.0
10	1.803639564276754E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.312738474968639E-4
17	0.0
18	2.7054593464151313E-4
19	0.0
20	4.5090989106918853E-4
21	0.0
22	0.0
23	9.01819782138377E-5
24	0.0010821837385660525
25	0.0
26	3.607279128553508E-4
27	4.5090989106918853E-4
28	0.0022545494553459426
29	5.410918692830263E-4
30	0.0019840035207044295
31	7.214558257107016E-4
32	8.116378039245394E-4
33	6.312738474968639E-4
34	5.410918692830263E-4
35	9.01819782138377E-5
36	9.01819782138377E-5
37	0.0013527296732075656
38	0.0
39	2.7054593464151313E-4
40	9.01819782138377E-5
41	8.116378039245394E-4
42	0.003787643084981184
43	0.0035170971503396702
44	0.0038778250631950214
45	0.001893821542490592
46	0.002164367477132105
47	8.116378039245394E-4
48	0.00315636923748432
49	0.0030661872592704824
50	0.0011723657167798903
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1108869.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.71081722220002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.46777108880161	45.861500123614604
2	12.750435019474082	14.71676049824814
3	3.499313384348688	6.058447053820359
4	1.3949682133718726	3.2201902237073226
5	0.7466168492026507	2.154393425967452
6	0.4288010497524696	1.4847875404171362
7	0.29160217375073627	1.1780019825647465
8	0.21113566744615248	0.974784953045769
9	0.1625178036335787	0.844113173476577
>10	0.8855006766862781	9.367338500014805
>50	0.08500713967338389	3.451375074014433
>100	0.06942031614968262	7.79443404520614
>500	0.005654141761887349	2.092069160323085
>1k	0.0012564759470860775	0.8018042455793948
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1221	0.11011219539909584	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1145	0.10325836505484416	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1123	0.10127436153413974	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1123	0.10127436153413974	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1116	0.10064308768664289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.01819782138377E-5
2	0.0	0.0	0.0	0.0	9.01819782138377E-5
3	0.0	0.0	0.0	0.0	9.01819782138377E-5
4	0.0	0.0	0.0	0.0	9.01819782138377E-5
5	0.0	0.0	0.0	0.0	9.01819782138377E-5
6	0.0	0.0	0.0	0.0	9.01819782138377E-5
7	0.0	0.0	0.0	0.0	9.01819782138377E-5
8	0.0	0.0	0.0	0.0	9.01819782138377E-5
9	0.0	0.0	0.0	0.0	9.01819782138377E-5
10	0.0	0.0	0.0	0.0	9.01819782138377E-5
11	0.0	0.0	0.0	0.0	9.01819782138377E-5
12	0.0	0.0	0.0	0.0	2.7054593464151313E-4
13	0.0	0.0	0.0	0.0	2.7054593464151313E-4
14	0.0	0.0	0.0	0.0	2.7054593464151313E-4
15	0.0	0.0	0.0	0.0	5.410918692830263E-4
16	0.0	0.0	0.0	2.7054593464151313E-4	5.410918692830263E-4
17	0.0	0.0	0.0	2.7054593464151313E-4	5.410918692830263E-4
18	0.0	0.0	0.0	2.7054593464151313E-4	7.214558257107016E-4
19	0.0	0.0	0.0	2.7054593464151313E-4	7.214558257107016E-4
20	0.0	0.0	0.0	4.5090989106918853E-4	9.920017603522147E-4
21	0.0	0.0	0.0	4.5090989106918853E-4	9.920017603522147E-4
22	0.0	0.0	0.0	5.410918692830263E-4	9.920017603522147E-4
23	0.0	0.0	0.0	8.116378039245393E-4	9.920017603522147E-4
24	0.0	0.0	0.0	0.0010821837385660525	9.920017603522147E-4
25	0.0	0.0	0.0	0.0016232756078490786	9.920017603522147E-4
26	0.0	0.0	0.0	0.0017134575860629163	9.920017603522147E-4
27	0.0	0.0	0.0	0.0018036395642767541	9.920017603522147E-4
28	0.0	0.0	0.0	0.00234473143355978	9.920017603522147E-4
29	0.0	0.0	0.0	0.0031563692374843196	9.920017603522147E-4
30	0.0	0.0	0.0	0.004509098910691885	9.920017603522147E-4
31	0.0	0.0	0.0	0.00820656001745923	9.920017603522147E-4
32	0.0	0.0	0.0	0.011723657167798902	9.920017603522147E-4
33	0.0	0.0	0.0	0.015601482230993923	9.920017603522147E-4
34	0.0	0.0	0.0	0.0212829468584657	0.00117236571677989
35	0.0	0.0	0.0	0.02507058994344688	0.00117236571677989
36	0.0	0.0	0.0	0.0322851482005539	0.00117236571677989
37	0.0	0.0	0.0	0.04355789547728361	0.00117236571677989
38	0.0	0.0	0.0	0.05537173462329635	0.00117236571677989
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1345	0.0	25.852371	1
CGGATCG	90	1.5128535E-8	24.443668	26
GGTATCA	725	0.0	23.373207	1
GTCTACG	50	0.0025753789	22.00724	1
ATCGATT	105	7.747258E-8	20.951715	29
GATTTCG	90	1.0001624E-5	19.559345	41
GCGTAGA	85	1.4270956E-4	18.12361	1
TATGCGA	85	1.4320339E-4	18.115438	12
ATACCGT	270	0.0	17.92374	6
CGTCGTA	260	0.0	17.767063	10
TACCGTC	250	0.0	17.597853	7
CTTACAC	250	0.0	17.597853	3
CCGTCGT	260	0.0	16.921013	9
GGTATAT	105	3.7632555E-5	16.767422	1
GTCCTAG	185	4.2018655E-10	16.654127	1
ACCGTCG	265	0.0	16.60175	8
CGAATGC	280	0.0	16.503197	43
GGATCGA	120	6.40751E-6	16.499475	27
GACGGAC	495	0.0	16.442438	7
TCAACGC	2090	0.0	16.313799	4
>>END_MODULE