FastQCFastQC Report
Thu 2 Feb 2017
SRR4062026_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062026_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806144
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC27490.15220270366039473No Hit
GTATCAACGCAGAGTACTTTTTTTT27330.15131683852450303No Hit
GGGTAGGCACACGCTGAGCCAGTCA23280.12889337727224406No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC20940.11593759965982779No Hit
TCGTAGTTCCGACCATAAACGATGC19710.10912751142766025No Hit
GAATAGGACCGCGGTTCTATTTTGT19590.10846311257574147No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19390.10735578115587682No Hit
ATCAGATACCGTCGTAGTTCCGACC19330.10702358172991744No Hit
TATCAACGCAGAGTACTTTTTTTTT18410.1019298571985401No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18360.10165302434357393No Hit
GATTAAGAGGGACGGCCGGGGGCAT18320.10143155805960101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.013.4307391
CCGTCGT3200.013.3614639
CGCAAGA5050.013.3533362
CAAGACG5350.013.317624
CGGTCCA4000.013.0645410
AAGACGG5600.012.893445
GTCTTAA1403.6379788E-1212.8873641
CGTCGTA3500.012.75913510
GCGCAAG5400.012.4854191
CGGACCA5350.012.4318489
TCGCGTA3050.012.1494399
GTATCAA23750.012.1148371
CGACCAT3850.012.09279810
GCGTAAC2950.011.91744611
GTCGTAG3600.011.87718511
TATAAGG802.8737737E-511.8722452
CCGACCA3450.011.8424299
GCAAGAC6100.011.6782653
CGAACGA1552.5465852E-1111.64536616
ACGGTAT4000.011.63931759
Produced by FastQC (version 0.11.5)