##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062026_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1806144
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244559127068495	32.0	32.0	32.0	32.0	32.0
2	30.831664031217887	32.0	32.0	32.0	32.0	32.0
3	30.85472476170228	32.0	32.0	32.0	32.0	32.0
4	30.85767081694483	32.0	32.0	32.0	32.0	32.0
5	30.730184857907233	32.0	32.0	32.0	32.0	32.0
6	34.40072441621488	36.0	36.0	36.0	32.0	36.0
7	34.33883953881861	36.0	36.0	36.0	32.0	36.0
8	34.28322824758159	36.0	36.0	36.0	32.0	36.0
9	34.42619912919457	36.0	36.0	36.0	32.0	36.0
10	34.09383526451933	36.0	36.0	36.0	32.0	36.0
11	34.40761312497785	36.0	36.0	36.0	32.0	36.0
12	34.21357599394068	36.0	36.0	36.0	32.0	36.0
13	34.32502170369583	36.0	36.0	36.0	32.0	36.0
14	34.20405128273271	36.0	36.0	36.0	32.0	36.0
15	34.13560934233372	36.0	36.0	36.0	32.0	36.0
16	34.14683380727118	36.0	36.0	36.0	32.0	36.0
17	34.072031355196486	36.0	36.0	36.0	32.0	36.0
18	34.07927607101095	36.0	36.0	36.0	32.0	36.0
19	34.08779587895539	36.0	36.0	36.0	32.0	36.0
20	34.06839709347649	36.0	36.0	36.0	32.0	36.0
21	34.04418861397541	36.0	36.0	36.0	32.0	36.0
22	34.02033337319727	36.0	36.0	36.0	32.0	36.0
23	33.97842475461536	36.0	36.0	36.0	32.0	36.0
24	33.9503826937387	36.0	36.0	36.0	32.0	36.0
25	33.609106472130684	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	28.0
5	134.0
6	360.0
7	89.0
8	328.0
9	251.0
10	129.0
11	39.0
12	69.0
13	73.0
14	219.0
15	317.0
16	489.0
17	735.0
18	1003.0
19	1407.0
20	2021.0
21	3016.0
22	4525.0
23	6709.0
24	9651.0
25	14157.0
26	19959.0
27	26392.0
28	35655.0
29	49104.0
30	65477.0
31	91052.0
32	130115.0
33	182281.0
34	386353.0
35	774005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.9875590075111	17.626123537339033	11.953429605686425	25.432887849463437
2	16.96960536303644	18.766032109342515	37.87937929541492	26.38498323220612
3	20.138217949619435	21.785328101529622	27.503290178519524	30.57316377033142
4	13.308629889100288	14.9815388636268	34.17010159715962	37.53972965011329
5	15.52259653887863	35.69598491754831	32.7342149891833	16.047203554389764
6	35.916805031321104	34.00817611839915	15.904262185369303	14.170756664910447
7	31.719810886759305	29.56723441284551	19.038194001806886	19.6747606985883
8	28.508338247748604	33.092711737397295	18.301744358768573	20.097205656085524
9	26.44106955381305	15.101643975029406	18.79856455670101	39.658721914456535
10	17.392883139548097	26.015397485642815	29.475646149436663	27.11607322537242
11	37.69972001219749	21.35624982674022	20.953400049898814	19.990630111163473
12	25.02868940160838	23.31658352746033	27.765242526618778	23.889484544312513
13	29.461242690804106	19.15868577497988	24.824878651271806	26.555192882944212
14	23.54228770784671	19.74037938156216	24.36790987609293	32.3494230344982
15	25.381297133736595	27.43160601338192	21.515409055553985	25.671687797327497
16	26.2501621355553	25.888419696615273	23.196636892850847	24.664781274978576
17	24.237413286354318	26.30789733060003	24.552670120614703	24.90201926243094
18	25.05064748408796	25.110454472138205	25.600772003456502	24.238126040317336
19	25.761902729831625	25.05102812740212	24.74549669501778	24.44157244774847
20	25.967096068441244	24.279616918791973	24.461271270128552	25.292015742638235
21	26.925250984764688	24.028535454853774	24.295757476342374	24.750456084039165
22	26.17369096628735	24.16089714172968	24.798537721941045	24.86687417004192
23	24.62203658060855	24.37373661252571	25.320113571884526	25.684113234981204
24	24.816293009048472	24.980049919534675	25.179439890407558	25.024217181009295
25	25.171733135905804	24.417027237906087	24.93394453113917	25.47729509504894
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	311.0
3	493.0
4	493.0
5	493.0
6	1004.5
7	1516.0
8	1516.0
9	1516.0
10	1816.5
11	2117.0
12	2117.0
13	2117.0
14	3017.0
15	3917.0
16	3917.0
17	3917.0
18	6685.0
19	9453.0
20	9453.0
21	9453.0
22	15459.5
23	21466.0
24	21466.0
25	21466.0
26	32931.0
27	44396.0
28	44396.0
29	44396.0
30	57342.5
31	70289.0
32	70289.0
33	70289.0
34	87796.5
35	105304.0
36	105304.0
37	105304.0
38	124233.5
39	143163.0
40	143163.0
41	143163.0
42	163972.0
43	184781.0
44	184781.0
45	184781.0
46	207393.5
47	230006.0
48	230006.0
49	230006.0
50	238692.5
51	247379.0
52	247379.0
53	247379.0
54	231477.5
55	215576.0
56	215576.0
57	215576.0
58	200262.0
59	184948.0
60	184948.0
61	184948.0
62	163185.5
63	141423.0
64	141423.0
65	141423.0
66	117617.0
67	93811.0
68	93811.0
69	93811.0
70	71775.0
71	49739.0
72	49739.0
73	49739.0
74	38872.5
75	28006.0
76	28006.0
77	28006.0
78	22304.5
79	16603.0
80	16603.0
81	16603.0
82	11522.5
83	6442.0
84	6442.0
85	6442.0
86	4821.0
87	3200.0
88	3200.0
89	3200.0
90	2094.5
91	989.0
92	989.0
93	989.0
94	625.5
95	262.0
96	262.0
97	262.0
98	499.0
99	736.0
100	736.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.965982778781758E-4
2	1.6609971297969596E-4
3	6.090322809255519E-4
4	0.010408915346727615
5	0.03333067573792566
6	0.06261959179334538
7	0.10707894830091066
8	0.10674674887495128
9	0.11638053222777364
10	0.12972387583714257
11	0.1380842280571206
12	0.1296685092661493
13	0.12468551787675844
14	0.1135014705361256
15	0.13105267354098013
16	0.11615906594380071
17	0.12280305446298856
18	0.11112070798341661
19	0.0967253995251763
20	0.08958311186704936
21	0.09146557528081925
22	0.10165302434357393
23	0.08149959250203749
24	0.09080117642890045
25	0.08858651358917119
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1806144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.02794379777131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.91099632678474	32.17859409775445
2	15.909848271601994	14.645952041527313
3	6.139817737862988	8.47809557300751
4	2.8913044697211725	5.3232319853828445
5	1.5203893056057067	3.4990196754576015
6	0.8983111881969572	2.4808450129943225
7	0.5761183139740698	1.8562278963525674
8	0.3867306827536767	1.4240334504527967
9	0.2753665147448044	1.140709901801582
>10	1.290228381240724	10.516694728444653
>50	0.09557162160605637	3.0685770229849134
>100	0.08985839956007699	8.645880067932438
>500	0.010748687381949609	3.4225444662446196
>1k	0.004710098965123985	3.3195940796623296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2749	0.15220270366039473	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2733	0.15131683852450303	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2328	0.12889337727224406	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2094	0.11593759965982779	No Hit
TCGTAGTTCCGACCATAAACGATGC	1971	0.10912751142766025	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1959	0.10846311257574147	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1939	0.10735578115587682	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1933	0.10702358172991744	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1841	0.1019298571985401	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1836	0.10165302434357393	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1832	0.10143155805960101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.536657099323199E-5
2	0.0	0.0	0.0	0.0	5.536657099323199E-5
3	0.0	0.0	0.0	0.0	5.536657099323199E-5
4	0.0	0.0	0.0	0.0	5.536657099323199E-5
5	0.0	0.0	0.0	0.0	5.536657099323199E-5
6	0.0	0.0	0.0	0.0	2.7683285496615996E-4
7	0.0	0.0	0.0	0.0	2.7683285496615996E-4
8	0.0	0.0	0.0	0.0	2.7683285496615996E-4
9	0.0	0.0	0.0	5.536657099323199E-5	2.7683285496615996E-4
10	0.0	0.0	0.0	5.536657099323199E-5	3.321994259593919E-4
11	0.0	0.0	0.0	5.536657099323199E-5	3.8756599695262395E-4
12	0.0	0.0	0.0	5.536657099323199E-5	9.965982778781758E-4
13	0.0	0.0	0.0	5.536657099323199E-5	0.0012180645618511039
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	905	0.0	13.430739	1
CCGTCGT	320	0.0	13.361463	9
CGCAAGA	505	0.0	13.353336	2
CAAGACG	535	0.0	13.31762	4
CGGTCCA	400	0.0	13.06454	10
AAGACGG	560	0.0	12.89344	5
GTCTTAA	140	3.6379788E-12	12.887364	1
CGTCGTA	350	0.0	12.759135	10
GCGCAAG	540	0.0	12.485419	1
CGGACCA	535	0.0	12.431848	9
TCGCGTA	305	0.0	12.149439	9
GTATCAA	2375	0.0	12.114837	1
CGACCAT	385	0.0	12.092798	10
GCGTAAC	295	0.0	11.917446	11
GTCGTAG	360	0.0	11.877185	11
TATAAGG	80	2.8737737E-5	11.872245	2
CCGACCA	345	0.0	11.842429	9
GCAAGAC	610	0.0	11.678265	3
CGAACGA	155	2.5465852E-11	11.645366	16
ACGGTAT	400	0.0	11.6393175	9
>>END_MODULE