FastQCFastQC Report
Thu 2 Feb 2017
SRR4062026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806144
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26600.1472750788419971No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC23020.12745384642642005No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG20820.115273200807909No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20710.11466416852698345No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20540.11372293682009851No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20120.11139754083838277No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19340.10707894830091066No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19110.10580551716806634No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19030.10536258460012048No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG18580.10287108890542503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24500.025.240151
GGTATCA12100.024.734621
CGCAATA4650.024.13152136
TACCGTA551.594724E-423.998497
AATACGA5100.022.4336939
TACGAAT5200.022.0028841
CAATACG5200.022.00227238
TAGCGGC5150.021.7856430
ATACGAA5400.021.18795840
GTAGGAC6050.021.0895823
TCTAGCG5550.020.21550428
CGAATGC5700.020.07224743
GCGCAAT5600.020.03667335
CTAGCGG5600.020.03500729
ATACCGT5750.019.89446
ACCGTCG6150.019.3158578
TACCGTC6000.019.0654667
TAGGACG2200.018.9988044
CGTCGTA6150.018.95815710
CGCTATC708.1288867E-418.85595712
Produced by FastQC (version 0.11.5)