##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1806144
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.915104775698946	32.0	32.0	32.0	32.0	32.0
2	31.3170096072074	32.0	32.0	32.0	32.0	32.0
3	31.49533536639382	32.0	32.0	32.0	32.0	32.0
4	31.60054015626661	32.0	32.0	32.0	32.0	32.0
5	31.51898353619645	32.0	32.0	32.0	32.0	32.0
6	35.20963112575741	36.0	36.0	36.0	36.0	36.0
7	35.204937147868606	36.0	36.0	36.0	36.0	36.0
8	35.13584298926332	36.0	36.0	36.0	36.0	36.0
9	35.26914299192091	36.0	36.0	36.0	36.0	36.0
10	35.111640600085046	36.0	36.0	36.0	36.0	36.0
11	35.27608706725488	36.0	36.0	36.0	36.0	36.0
12	35.176652581411005	36.0	36.0	36.0	36.0	36.0
13	35.230646615109315	36.0	36.0	36.0	36.0	36.0
14	35.18516353070409	36.0	36.0	36.0	36.0	36.0
15	35.15226803621417	36.0	36.0	36.0	36.0	36.0
16	35.16661683675277	36.0	36.0	36.0	36.0	36.0
17	35.13905646504376	36.0	36.0	36.0	36.0	36.0
18	35.148641525814114	36.0	36.0	36.0	36.0	36.0
19	35.14987896867581	36.0	36.0	36.0	36.0	36.0
20	35.142644218844126	36.0	36.0	36.0	36.0	36.0
21	35.126820452854254	36.0	36.0	36.0	36.0	36.0
22	35.11698845717728	36.0	36.0	36.0	36.0	36.0
23	35.07977326281847	36.0	36.0	36.0	36.0	36.0
24	35.0647130018426	36.0	36.0	36.0	36.0	36.0
25	35.045847950108076	36.0	36.0	36.0	36.0	36.0
26	35.00327936199993	36.0	36.0	36.0	36.0	36.0
27	34.997577712519046	36.0	36.0	36.0	36.0	36.0
28	34.965086393997375	36.0	36.0	36.0	36.0	36.0
29	34.94247302540661	36.0	36.0	36.0	36.0	36.0
30	34.92760156443783	36.0	36.0	36.0	32.0	36.0
31	34.929219375642255	36.0	36.0	36.0	32.0	36.0
32	34.90407520109139	36.0	36.0	36.0	32.0	36.0
33	34.87476469207328	36.0	36.0	36.0	32.0	36.0
34	34.86836265546933	36.0	36.0	36.0	32.0	36.0
35	34.85989766043018	36.0	36.0	36.0	32.0	36.0
36	34.82681668792743	36.0	36.0	36.0	32.0	36.0
37	34.82043735161759	36.0	36.0	36.0	32.0	36.0
38	34.80119303887176	36.0	36.0	36.0	32.0	36.0
39	34.780626018744904	36.0	36.0	36.0	32.0	36.0
40	34.775648010346906	36.0	36.0	36.0	32.0	36.0
41	34.74325967364729	36.0	36.0	36.0	32.0	36.0
42	34.71346027780731	36.0	36.0	36.0	32.0	36.0
43	34.70694972272421	36.0	36.0	36.0	32.0	36.0
44	34.64628124889267	36.0	36.0	36.0	32.0	36.0
45	34.64283523351405	36.0	36.0	36.0	32.0	36.0
46	34.61471898143227	36.0	36.0	36.0	32.0	36.0
47	34.58877808192481	36.0	36.0	36.0	32.0	36.0
48	34.56122767619858	36.0	36.0	36.0	32.0	36.0
49	34.550436731511994	36.0	36.0	36.0	32.0	36.0
50	34.11420185765919	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	15.0
21	37.0
22	103.0
23	310.0
24	793.0
25	1829.0
26	3937.0
27	7624.0
28	13106.0
29	22172.0
30	33946.0
31	52700.0
32	83265.0
33	147934.0
34	337412.0
35	1100957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21512832157375	18.564648154096655	12.490169581639547	25.730053942690045
2	16.463960222805444	19.21281684993854	37.41381792409997	26.90940500315604
3	19.507933229871266	22.178273298204953	27.82579616053489	30.48799731138889
4	13.071549112363135	15.481711314269516	34.35567706672336	37.09106250664399
5	15.169056287870736	36.06279455015768	32.50205963644095	16.26608952553063
6	36.11809963369315	34.202186324839325	15.70701209654201	13.97270194492552
7	31.761421016264485	29.491834538109917	19.03663273803196	19.710111707593637
8	28.47447379610928	33.286659313986036	18.16959223627795	20.06927465362673
9	26.4978872116509	14.79361556996563	18.703602813507672	40.0048944048758
10	17.31557246574607	26.13525064183733	29.549044566238663	27.00013232617794
11	37.98323943162893	21.2475306509337	20.864836912228483	19.904393005208888
12	25.03477020658375	23.324662928315792	27.723315527444104	23.917251337656356
13	29.639331083235888	18.994775610361078	24.820003277701	26.54589002870203
14	23.598450621877326	19.627670883384713	24.25105639417455	32.52282210056341
15	25.472664416569224	27.355072463768117	21.487046437050424	25.68521668261224
16	26.386772646135174	25.82269552237484	23.127051340541353	24.663480490948633
17	24.36057147159916	26.250343272740157	24.465546490202332	24.923538765458346
18	25.15058348157757	25.06487588731998	25.521207923412405	24.26333270769004
19	25.83467966527549	24.98568772554982	24.74439994197577	24.435232667198925
20	26.050874268259534	24.243649021476198	24.525742481614095	25.179734228650176
21	27.022002133828828	23.956907066605467	24.28384684933585	24.737243950229853
22	26.250786205308103	24.10322764962262	24.825318468516354	24.820667676552922
23	24.69094380071578	24.21351785904114	25.36846452995996	25.727073810283123
24	24.934957508525148	24.837566793475762	25.132119723585326	25.095355974413756
25	25.27608632341493	24.237073800562307	25.036292907850893	25.450546968171867
26	24.63648876550688	25.503810902181122	25.44130169743773	24.418398634874265
27	25.3175973720738	24.665377360214247	25.00300364479146	25.014021622920495
28	24.91065056355894	24.66935863853329	25.177079371090706	25.242911426817066
29	24.64648287836575	24.869555780960017	25.362984847209734	25.120976493464504
30	24.634976986566222	25.123372269490545	25.50081140832913	24.740839335614098
31	25.546691943463834	24.671065744772267	24.56033211231048	25.22191019945342
32	25.28576277136044	24.660503584734442	24.472366622354635	25.581367021550484
33	24.673959202475558	24.562007686008663	25.23067161497537	25.533361496540408
34	25.492061189495534	24.650647490458866	25.181784005222223	24.675507314823374
35	25.630197897282546	24.425971644485834	25.410059801598877	24.53377065663274
36	24.511348215506747	25.110138012327926	24.912811444055095	25.465702328110236
37	25.700605221034962	24.72879663942037	24.725751432493023	24.844846707051644
38	24.89142615428227	24.41787587257716	25.163663583855993	25.527034389284577
39	25.76056137615558	24.373952527305576	24.64597014727044	25.219515949268413
40	25.7510760504988	24.64341120465611	24.971624600945	24.633888143900094
41	24.73166323944177	25.286164974093722	25.47457929291755	24.507592493546962
42	26.094631145612148	25.10419060222085	24.638648110671543	24.162530141495463
43	25.374293769669805	24.298863608679184	25.051548446680854	25.27529417497016
44	24.75806692734201	24.916199632574006	24.796160520199017	25.52957291988497
45	24.980081512155124	25.21832822013708	24.731536433807225	25.070053833900573
46	24.794876948316023	24.8928775695403	24.7235081343284	25.588737347815275
47	25.124312688282302	24.69721188551661	25.239586718792822	24.938888707408267
48	25.821540355984673	25.04931927496375	24.137957763986158	24.991182605065422
49	25.108065713698807	25.215148634305184	24.388052115164523	25.288733536831487
50	25.2196835095766	25.33639772750552	24.52687278427082	24.917045978647057
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	24.5
2	25.0
3	91.5
4	158.0
5	159.5
6	161.0
7	128.0
8	95.0
9	148.0
10	201.0
11	373.5
12	546.0
13	1056.0
14	1566.0
15	2043.5
16	2521.0
17	2736.5
18	2952.0
19	2982.0
20	3012.0
21	3562.5
22	4113.0
23	4948.0
24	5783.0
25	7406.5
26	9030.0
27	11924.0
28	14818.0
29	17526.5
30	20235.0
31	23755.5
32	27276.0
33	31797.5
34	36319.0
35	42120.5
36	47922.0
37	55712.5
38	63503.0
39	70962.5
40	78422.0
41	84637.5
42	90853.0
43	94626.0
44	98399.0
45	107319.5
46	116240.0
47	123305.0
48	130370.0
49	137355.0
50	144340.0
51	139107.0
52	133874.0
53	133556.5
54	133239.0
55	133230.5
56	133222.0
57	128241.0
58	123260.0
59	113574.5
60	103889.0
61	91869.5
62	79850.0
63	69020.5
64	58191.0
65	50205.0
66	42219.0
67	37302.5
68	32386.0
69	29224.0
70	26062.0
71	20319.0
72	14576.0
73	12909.5
74	11243.0
75	8113.5
76	4984.0
77	4311.5
78	3639.0
79	3197.5
80	2756.0
81	2192.0
82	1628.0
83	1378.0
84	1128.0
85	893.0
86	658.0
87	466.0
88	274.0
89	178.0
90	82.0
91	58.5
92	35.0
93	30.5
94	26.0
95	18.5
96	11.0
97	13.5
98	16.0
99	14.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029012083200453564
2	0.004650791963431487
3	3.8756599695262395E-4
4	0.0
5	0.0
6	4.4293256794585587E-4
7	0.0
8	0.0
9	0.0
10	5.536657099323198E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.4293256794585587E-4
17	0.0
18	1.6609971297969596E-4
19	1.1073314198646397E-4
20	2.7683285496615996E-4
21	5.536657099323198E-5
22	0.0
23	0.0
24	9.412317068849438E-4
25	3.321994259593919E-4
26	5.536657099323199E-4
27	2.7683285496615996E-4
28	0.0018270968427766558
29	4.4293256794585587E-4
30	0.0013841642748307998
31	4.4293256794585587E-4
32	7.197654229120159E-4
33	2.7683285496615996E-4
34	9.412317068849438E-4
35	2.7683285496615996E-4
36	5.536657099323198E-5
37	0.0014948974168172639
38	0.0
39	6.090322809255519E-4
40	1.1073314198646397E-4
41	9.965982778781758E-4
42	0.004373959108465327
43	0.004207859395485632
44	0.0036541936855533116
45	0.0018270968427766558
46	0.0018270968427766558
47	7.197654229120159E-4
48	0.0030451614046277595
49	0.0037095602565465438
50	0.0012734311328443358
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1806144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.475587951988715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.40829426907928	32.95199579169188
2	16.357873314134224	15.53199306465337
3	6.167607316032347	8.784323507568683
4	2.940518248685528	5.584113309595905
5	1.5473902996071969	3.6731632132527823
6	0.9296375512699654	2.64810535972732
7	0.5863236128491587	1.9485240775103612
8	0.38763521823934327	1.4722567917448226
9	0.2868432200349316	1.2256245469080047
>10	1.212191853478642	10.042544389666595
>50	0.0872655870874744	2.871593728496099
>100	0.0751865768580499	7.412777561259467
>500	0.009368447889435806	3.066095319919979
>1k	0.0038644847543922705	2.786889338004838
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2660	0.1472750788419971	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2302	0.12745384642642005	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2082	0.115273200807909	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2071	0.11466416852698345	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2054	0.11372293682009851	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2012	0.11139754083838277	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1934	0.10707894830091066	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1911	0.10580551716806634	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1903	0.10536258460012048	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1858	0.10287108890542503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1073314198646398E-4
2	0.0	0.0	0.0	0.0	1.1073314198646398E-4
3	0.0	0.0	0.0	0.0	1.1073314198646398E-4
4	0.0	0.0	0.0	0.0	1.1073314198646398E-4
5	0.0	0.0	0.0	0.0	2.2146628397292796E-4
6	0.0	0.0	0.0	0.0	3.321994259593919E-4
7	0.0	0.0	0.0	0.0	3.321994259593919E-4
8	0.0	0.0	0.0	0.0	3.321994259593919E-4
9	0.0	0.0	0.0	5.536657099323199E-5	3.321994259593919E-4
10	0.0	0.0	0.0	5.536657099323199E-5	3.8756599695262395E-4
11	0.0	0.0	0.0	5.536657099323199E-5	3.8756599695262395E-4
12	0.0	0.0	0.0	5.536657099323199E-5	0.0011626979908578717
13	0.0	0.0	0.0	5.536657099323199E-5	0.0013287977038375677
14	0.0	0.0	0.0	5.536657099323199E-5	0.0013287977038375677
15	0.0	0.0	0.0	5.536657099323199E-5	0.0014395308458240318
16	0.0	0.0	0.0	1.6609971297969596E-4	0.0014948974168172637
17	0.0	0.0	0.0	2.7683285496615996E-4	0.0014948974168172637
18	0.0	0.0	0.0	3.321994259593919E-4	0.0016056305588037277
19	0.0	0.0	0.0	3.321994259593919E-4	0.0016056305588037277
20	0.0	0.0	0.0	3.8756599695262395E-4	0.0018824634137698877
21	0.0	0.0	0.0	3.8756599695262395E-4	0.0019378299847631196
22	0.0	0.0	0.0	6.643988519187839E-4	0.0019378299847631196
23	0.0	0.0	0.0	7.751319939052479E-4	0.0019378299847631196
24	0.0	0.0	0.0	0.001051964848871408	0.0019378299847631196
25	0.0	0.0	0.0	0.0012180645618511039	0.0019378299847631196
26	0.0	0.0	0.0	0.0017717302717834237	0.0020485631267495837
27	0.0	0.0	0.0	0.0020485631267495837	0.0020485631267495837
28	0.0	0.0	0.0	0.0025468622656886716	0.0020485631267495837
29	0.0	0.0	0.0	0.0035988271145600795	0.0020485631267495837
30	0.0	0.0	0.0	0.005758123383296127	0.0020485631267495837
31	0.0	0.0	0.0	0.01085184791467347	0.0021592962687360475
32	0.0	0.0	0.0	0.016222405301016974	0.0021592962687360475
33	0.0	0.0	0.0	0.020928563835441692	0.002270029410722512
34	0.0	0.0	0.0	0.027627918925622764	0.0023253959817157435
35	0.0	0.0	0.0	0.0356560717196414	0.0023253959817157435
36	0.0	0.0	0.0	0.04595425392438255	0.0023253959817157435
37	0.0	0.0	0.0	0.06356082350023033	0.0023253959817157435
38	0.0	0.0	0.0	0.08559671875553666	0.0023807625527089756
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2450	0.0	25.24015	1
GGTATCA	1210	0.0	24.73462	1
CGCAATA	465	0.0	24.131521	36
TACCGTA	55	1.594724E-4	23.99849	7
AATACGA	510	0.0	22.43369	39
TACGAAT	520	0.0	22.00288	41
CAATACG	520	0.0	22.002272	38
TAGCGGC	515	0.0	21.78564	30
ATACGAA	540	0.0	21.187958	40
GTAGGAC	605	0.0	21.089582	3
TCTAGCG	555	0.0	20.215504	28
CGAATGC	570	0.0	20.072247	43
GCGCAAT	560	0.0	20.036673	35
CTAGCGG	560	0.0	20.035007	29
ATACCGT	575	0.0	19.8944	6
ACCGTCG	615	0.0	19.315857	8
TACCGTC	600	0.0	19.065466	7
TAGGACG	220	0.0	18.998804	4
CGTCGTA	615	0.0	18.958157	10
CGCTATC	70	8.1288867E-4	18.855957	12
>>END_MODULE