FastQCFastQC Report
Thu 2 Feb 2017
SRR4062025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850411
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC49950.2699400295393834No Hit
GTCCTACAGTGGACATTTCTAAATT43360.23432631993648978No Hit
CTGTAGGACGTGGAATATGGCAAGA42560.2300029561000232No Hit
GGGTAGGCACACGCTGAGCCAGTCA41200.22265323757802996No Hit
TCGTAGTTCCGACCATAAACGATGC39560.2137903417132734No Hit
ATCAGATACCGTCGTAGTTCCGACC38740.20935889378089514No Hit
GATTAAGAGGGACGGCCGGGGGCAT36160.19541604540829036No Hit
ACCATACTCCCCCCGGAACCCAAAG35750.19320032144210125No Hit
GAATAGGACCGCGGTTCTATTTTGT35730.19309223734618958No Hit
GTCCTAAAGTGTGTATTTCTCATTT35340.19098459747591212No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG34930.188768873509723No Hit
GTCCTACAGTGTGCATTTCTCATTT33720.18222978570706724No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC33550.18131107089181808No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT33070.17871705258993814No Hit
GAACTACGACGGTATCTGATCGTCT31530.1703945772047399No Hit
GTGCATGGCCGTTCTTAGTTGGTGG31190.16855714757424162No Hit
CTTTAGGACGTGAAATATGGCGAGG31020.16763843275899246No Hit
GTCTTGCGCCGGTCCAAGAATTTCA30970.1673682225192133No Hit
GTATCTGATCGTCTTCGAACCTCCG30390.164233783737775No Hit
GCCATGCACCACCACCCACGGAATC27570.14899392621423024No Hit
GAGTATGGTTGCAAAGCTGAAACTT27030.14607565562461527No Hit
GTTATTGCTCAATCTCGGGTGGCTG26090.14099570311676704No Hit
GTATCAACGCAGAGTACATGGGGAA26050.1407795349249437No Hit
GTACATGGGGAATAATTGCAATCCC24810.13407832097842046No Hit
GAATAACGCCGCCGCATCGCCAGTC23700.12807965365532306No Hit
CTGTAGGACCTGGAATATGGCGAGA23670.12791752751145558No Hit
GTATCAACGCAGAGTACTTTTTTTT23500.12699881269620641No Hit
CTCGGGGGTCGCGTAACTAGTTAGC23410.12651243426460393No Hit
GCTTTGAACACTCTAATTTTTTCAA22270.120351640797639No Hit
CATCTAAGGGCATCACAGACCTGTT22090.11937888393443402No Hit
GGATTGACAGATTGATAGCTCTTTC22010.11894654755078737No Hit
GTCCTATTCCATTATTCCTAGCTGC21580.11662273948868658No Hit
TCACAGACCTGTTATTGCTCAATCT21480.11608231900912824No Hit
GCCCCGGACATCTAAGGGCATCACA21370.1154878564816141No Hit
ACCATAAACGATGCCGACTGGCGAT20070.10846239024735586No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG19820.10711133904846004No Hit
CTCCACTCCTGGTGGTGCCCTTCCG19660.10624666628116673No Hit
ATCTCGGGTGGCTGAACGCCACTTG19620.1060304980893434No Hit
CTATTGGAGCTGGAATTACCGCGGC19560.1057062458016084No Hit
GAATAATGGAATAGGACCGCGGTTC19420.10494965713022675No Hit
TCTAAGGGCATCACAGACCTGTTAT19250.1040309423149776No Hit
GCGTTATTCCCATGACCCGCCGGGC19100.10322031159564009No Hit
GGAGTGGAGCCTGCGGCTTAATTTG18920.10224755473243512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10300.016.2264921
CGGTCCA6100.013.8618410
CCGGTCC6550.013.0549049
GCCGGTC6100.012.9273348
AGGACCT13650.012.8765595
GGTCCAA6800.012.71466611
AATCGTA751.4776415E-512.66774913
CCAACGA1052.0025254E-812.662950519
CGCCGGT6050.012.5633977
AAGACGG8700.012.5585445
CGGTTCT6250.012.46540312
GATATAC5300.012.3629581
GAATTTC7000.012.34838218
CAAGACG8700.012.3398014
TGTAGGA24300.012.2346912
AGGCCCG5750.012.22713210
GGACCTG14000.012.1477966
GTATCAA27600.012.1110771
TGCACCG951.0390413E-612.0010265
GACTATA951.0451713E-611.9951831
Produced by FastQC (version 0.11.5)