##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062025_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1850411
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.374163901965563	32.0	32.0	32.0	32.0	32.0
2	31.103217609493242	32.0	32.0	32.0	32.0	32.0
3	31.10558519161419	32.0	32.0	32.0	32.0	32.0
4	31.112452314647935	32.0	32.0	32.0	32.0	32.0
5	31.108607222935877	32.0	32.0	32.0	32.0	32.0
6	34.684987281204016	36.0	36.0	36.0	32.0	36.0
7	34.637427576900485	36.0	36.0	36.0	32.0	36.0
8	34.629704427827114	36.0	36.0	36.0	32.0	36.0
9	34.706480344096526	36.0	36.0	36.0	32.0	36.0
10	34.52587668361245	36.0	36.0	36.0	32.0	36.0
11	34.63704225709856	36.0	36.0	36.0	32.0	36.0
12	34.5549323906959	36.0	36.0	36.0	32.0	36.0
13	34.61232558604548	36.0	36.0	36.0	32.0	36.0
14	34.553487846754045	36.0	36.0	36.0	32.0	36.0
15	34.519111159628864	36.0	36.0	36.0	32.0	36.0
16	34.529826616897545	36.0	36.0	36.0	32.0	36.0
17	34.45271347824889	36.0	36.0	36.0	32.0	36.0
18	34.461933592050634	36.0	36.0	36.0	32.0	36.0
19	34.433030823962895	36.0	36.0	36.0	32.0	36.0
20	34.4180487470081	36.0	36.0	36.0	32.0	36.0
21	34.37193304622595	36.0	36.0	36.0	32.0	36.0
22	34.3357999925422	36.0	36.0	36.0	32.0	36.0
23	34.33608911749876	36.0	36.0	36.0	32.0	36.0
24	34.30441561361233	36.0	36.0	36.0	32.0	36.0
25	33.921807641653665	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	25.0
5	141.0
6	396.0
7	60.0
8	331.0
9	267.0
10	152.0
11	35.0
12	72.0
13	87.0
14	196.0
15	298.0
16	615.0
17	815.0
18	1080.0
19	1485.0
20	1993.0
21	2627.0
22	3760.0
23	5213.0
24	7170.0
25	10285.0
26	14468.0
27	19242.0
28	26446.0
29	37020.0
30	50833.0
31	73299.0
32	110609.0
33	168709.0
34	413276.0
35	899405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.632954044947155	19.04641981847119	12.191937397150337	25.12868873943131
2	15.700545879906421	19.780188898982765	39.777789426866626	24.741475794244185
3	19.101046478699697	23.639210201492006	28.35576817070508	28.903975149103218
4	11.918792403718259	16.28551400075991	36.665565897263086	35.130127698258754
5	14.06447574064173	37.40918519543665	33.67566214595133	14.85067691797029
6	33.6632180278083	36.51829516253234	16.9198348287289	12.898651980930461
7	30.400916231570278	30.097038531389426	20.633766509182603	18.868278727857692
8	26.946034569600414	35.28460219640151	19.21469908628442	18.554664147713655
9	26.42829741634685	14.479679384827481	19.952526705757016	39.13949649306865
10	17.3703432530438	26.228058640527248	30.197728602219666	26.203869504209287
11	37.05318197856529	21.09176965155067	23.150763809066135	18.704284560817904
12	25.184960313079692	24.102417579563237	28.88140678065157	21.8312153267055
13	29.245331513790234	19.92102086324045	25.491604002634084	25.342043620335236
14	22.51263350394425	22.084825727981993	24.594050620583687	30.80849014749007
15	24.057137507623256	30.738007200360613	21.50064692853699	23.70420836347914
16	24.3594412049404	26.316760551263368	26.40538775483703	22.918410488959204
17	22.62212090533719	28.192848141368437	25.93610651331335	23.24892443998102
18	23.65367770502074	25.10976225347114	29.11741680115868	22.11914324034944
19	25.11237058246613	25.172416964238863	25.583220768907445	24.131991684387565
20	25.868372696100256	24.931044611256052	25.283083575716237	23.91749911692746
21	26.53410397120115	24.78751112277105	24.10886657272602	24.56951833330178
22	25.770572400481257	26.485910707948516	25.298945737507683	22.444571154062544
23	22.631666625199987	26.545021456302592	26.91064940066224	23.91266251783518
24	24.454786953509014	25.777081064824053	26.92892932853207	22.83920265313486
25	23.61534296546356	26.09394599465691	26.47020164511779	23.82050939476174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	83.0
2	301.0
3	519.0
4	519.0
5	519.0
6	1120.0
7	1721.0
8	1721.0
9	1721.0
10	2142.5
11	2564.0
12	2564.0
13	2564.0
14	3797.0
15	5030.0
16	5030.0
17	5030.0
18	8887.5
19	12745.0
20	12745.0
21	12745.0
22	22760.0
23	32775.0
24	32775.0
25	32775.0
26	53100.5
27	73426.0
28	73426.0
29	73426.0
30	93299.5
31	113173.0
32	113173.0
33	113173.0
34	133424.0
35	153675.0
36	153675.0
37	153675.0
38	165209.5
39	176744.0
40	176744.0
41	176744.0
42	192662.5
43	208581.0
44	208581.0
45	208581.0
46	221966.5
47	235352.0
48	235352.0
49	235352.0
50	229084.0
51	222816.0
52	222816.0
53	222816.0
54	194125.0
55	165434.0
56	165434.0
57	165434.0
58	156339.5
59	147245.0
60	147245.0
61	147245.0
62	133345.0
63	119445.0
64	119445.0
65	119445.0
66	99641.5
67	79838.0
68	79838.0
69	79838.0
70	60932.5
71	42027.0
72	42027.0
73	42027.0
74	34029.0
75	26031.0
76	26031.0
77	26031.0
78	22439.5
79	18848.0
80	18848.0
81	18848.0
82	12874.0
83	6900.0
84	6900.0
85	6900.0
86	5200.5
87	3501.0
88	3501.0
89	3501.0
90	2301.0
91	1101.0
92	1101.0
93	1101.0
94	659.0
95	217.0
96	217.0
97	217.0
98	418.5
99	620.0
100	620.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.646727672933203E-4
2	2.1616819182333007E-4
3	8.106307193374877E-4
4	0.010267989111608177
5	0.034046490212174485
6	0.06371557453992653
7	0.10646283447299007
8	0.10938110506260501
9	0.11964909417421318
10	0.13370002664272962
11	0.13872593710262207
12	0.13224089134792216
13	0.1258098876411781
14	0.11624444515299574
15	0.132294933395878
16	0.1199733464619482
17	0.12451287849023812
18	0.11338021661133661
19	0.09943736823873184
20	0.09468166801861856
21	0.09327657477176693
22	0.10349052183541926
23	0.08306262770811458
24	0.09349274296359025
25	0.09014213599032864
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1850411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.691324479725836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.58118317968092	23.097894299303746
2	16.43969287417408	11.406294396900194
3	7.071082411528218	7.35915643083623
4	3.4431662726504477	4.777919936086594
5	1.9150609304252584	3.321800006791416
6	1.1305154281759837	2.3531446528913547
7	0.727381605509936	1.7663681908130386
8	0.5024865938581795	1.3945540379397052
9	0.34885831457876926	1.089212128965212
>10	1.4934464433530685	9.070991479442114
>50	0.13836067398667493	3.423356144843536
>100	0.16237315420829135	12.178896152133248
>500	0.028644797611069386	6.963750752220623
>1k	0.01774732025903212	11.796661390833075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	4995	0.2699400295393834	No Hit
GTCCTACAGTGGACATTTCTAAATT	4336	0.23432631993648978	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4256	0.2300029561000232	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4120	0.22265323757802996	No Hit
TCGTAGTTCCGACCATAAACGATGC	3956	0.2137903417132734	No Hit
ATCAGATACCGTCGTAGTTCCGACC	3874	0.20935889378089514	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3616	0.19541604540829036	No Hit
ACCATACTCCCCCCGGAACCCAAAG	3575	0.19320032144210125	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3573	0.19309223734618958	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3534	0.19098459747591212	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3493	0.188768873509723	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3372	0.18222978570706724	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	3355	0.18131107089181808	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	3307	0.17871705258993814	No Hit
GAACTACGACGGTATCTGATCGTCT	3153	0.1703945772047399	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	3119	0.16855714757424162	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3102	0.16763843275899246	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3097	0.1673682225192133	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3039	0.164233783737775	No Hit
GCCATGCACCACCACCCACGGAATC	2757	0.14899392621423024	No Hit
GAGTATGGTTGCAAAGCTGAAACTT	2703	0.14607565562461527	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	2609	0.14099570311676704	No Hit
GTATCAACGCAGAGTACATGGGGAA	2605	0.1407795349249437	No Hit
GTACATGGGGAATAATTGCAATCCC	2481	0.13407832097842046	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2370	0.12807965365532306	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2367	0.12791752751145558	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2350	0.12699881269620641	No Hit
CTCGGGGGTCGCGTAACTAGTTAGC	2341	0.12651243426460393	No Hit
GCTTTGAACACTCTAATTTTTTCAA	2227	0.120351640797639	No Hit
CATCTAAGGGCATCACAGACCTGTT	2209	0.11937888393443402	No Hit
GGATTGACAGATTGATAGCTCTTTC	2201	0.11894654755078737	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2158	0.11662273948868658	No Hit
TCACAGACCTGTTATTGCTCAATCT	2148	0.11608231900912824	No Hit
GCCCCGGACATCTAAGGGCATCACA	2137	0.1154878564816141	No Hit
ACCATAAACGATGCCGACTGGCGAT	2007	0.10846239024735586	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1982	0.10711133904846004	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	1966	0.10624666628116673	No Hit
ATCTCGGGTGGCTGAACGCCACTTG	1962	0.1060304980893434	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1956	0.1057062458016084	No Hit
GAATAATGGAATAGGACCGCGGTTC	1942	0.10494965713022675	No Hit
TCTAAGGGCATCACAGACCTGTTAT	1925	0.1040309423149776	No Hit
GCGTTATTCCCATGACCCGCCGGGC	1910	0.10322031159564009	No Hit
GGAGTGGAGCCTGCGGCTTAATTTG	1892	0.10224755473243512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.404204795583252E-5	0.0
5	0.0	0.0	0.0	5.404204795583252E-5	0.0
6	0.0	0.0	0.0	5.404204795583252E-5	0.0
7	0.0	0.0	0.0	5.404204795583252E-5	0.0
8	0.0	0.0	0.0	2.1616819182333007E-4	0.0
9	0.0	0.0	0.0	4.8637843160249266E-4	0.0
10	0.0	0.0	0.0	4.8637843160249266E-4	0.0
11	0.0	0.0	0.0	4.8637843160249266E-4	0.0
12	0.0	0.0	0.0	4.8637843160249266E-4	0.0
13	0.0	0.0	0.0	5.404204795583251E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1030	0.0	16.226492	1
CGGTCCA	610	0.0	13.86184	10
CCGGTCC	655	0.0	13.054904	9
GCCGGTC	610	0.0	12.927334	8
AGGACCT	1365	0.0	12.876559	5
GGTCCAA	680	0.0	12.714666	11
AATCGTA	75	1.4776415E-5	12.667749	13
CCAACGA	105	2.0025254E-8	12.6629505	19
CGCCGGT	605	0.0	12.563397	7
AAGACGG	870	0.0	12.558544	5
CGGTTCT	625	0.0	12.465403	12
GATATAC	530	0.0	12.362958	1
GAATTTC	700	0.0	12.348382	18
CAAGACG	870	0.0	12.339801	4
TGTAGGA	2430	0.0	12.234691	2
AGGCCCG	575	0.0	12.227132	10
GGACCTG	1400	0.0	12.147796	6
GTATCAA	2760	0.0	12.111077	1
TGCACCG	95	1.0390413E-6	12.001026	5
GACTATA	95	1.0451713E-6	11.995183	1
>>END_MODULE