FastQCFastQC Report
Thu 2 Feb 2017
SRR4062024_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062024_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290719
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCG12940.4451033472184481No Hit
GTATCAACGCAGAGTACTTTTTTTT11840.40726612295721987No Hit
TTGTAGAACAGTGTATATCAATGAG10910.3752764697181815No Hit
GTCCTAAAGTGTGTATTTCTCATTT9600.33021577537071883No Hit
GTGTATATCAATGAGTTACAATGAA9370.32230435575246197No Hit
CTGTAGGACGTGGAATATGGCAAGA9040.31095318847409353No Hit
CCATAGGGTCTTCTCGTCTTATTAT8960.30820139034600424No Hit
GTCCTACAGTGGACATTTCTAAATT8950.307857415579993No Hit
CTTTAGGACGTGAAATATGGCGAGG8260.28412315672522265No Hit
TATCAACGCAGAGTACTTTTTTTTT7760.2669244184246644No Hit
GTCCTACAGTGTGCATTTCTCATTT7410.2548853016142736No Hit
GTGTATATCAATGAGTTACAATGAG7330.25213350348618424No Hit
CTGTAGGACCTGGAATATGGCGAGA7070.24319015956989395No Hit
GTTCTACAAATCCCGTTTCCAACGA6690.23011911846146965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6210.21360832969293375No Hit
GTTCTACAGTGTGGTTTTTATCATT5910.20328908671259877No Hit
GATATACACTGTTCTACAATGCCGG5880.20225716241456526No Hit
GTATTAGAGGCACTGCCTGCCCAGT5680.19537766709434196No Hit
GGTATCAACGCAGAGTACTTTTTTT5050.17370725683563853No Hit
GTCAGGATACCGCGGCCGTTAAACT4650.15994826619519192No Hit
ACCTATAACTTCTCTGTTAACCCAA4630.1592603166631696No Hit
CTGTAGAACATATTAGATGAGTGAG4510.15513261947103563No Hit
ATAAATAATCCACCTATAACTTCTC4290.14756517461878996No Hit
CTCTAATACTTGTAATGCTAGAGGT4250.14618927555474528No Hit
CCTATAACTTCTCTGTTAACCCAAC4170.14343747742665597No Hit
CCATTGGGATGTCCTGATCCAACAT4090.14068567929856668No Hit
GTTCATGCTAGTCCCTAATTAAGGA4090.14068567929856668No Hit
GTTATATAATTTAAGCTCCATAGGG4050.139309780234522No Hit
CTGTTAGTATGAGTAACAAGAATTC4010.13793388117047733No Hit
CTTCTACACCATTGGGATGTCCTGA3980.13690195687244383No Hit
GGTCAGGATACCGCGGCCGTTAAAC3970.1365579821064327No Hit
GACTATAGGCAATAATCACACTATA3930.13518208304238802No Hit
CACTATAAATAATCCACCTATAACT3900.13415015874435451No Hit
GCCTAAAGGAAAGATCCAAAAAGAT3810.13105438585025403No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3790.1303664363182317No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3760.12933451202019822No Hit
ATCCTGACCGTGCAAAGGTAGCATA3680.12658271389210887No Hit
GTATCAACGCAGAGTACATGGGAGA3650.12555078959407537No Hit
GTACATGGGAGAAATCGTAAATAGA3630.12486284006205306No Hit
ATGTTCTACAGTGTGGTTTTTATCA3600.12383091576401956No Hit
CCTCTAGCATTACAAGTATTAGAGG3530.1214230924019414No Hit
CTGTTAACCCAACACCGGAATGCCT3520.12107911763593023No Hit
GTAGAACAGTGTATATCAATGAGTT3520.12107911763593023No Hit
GATTAAACCTTGTACCTTTTGCATA3520.12107911763593023No Hit
ATCTATAACTTTATAGATGCAACAC3400.11695142044379624No Hit
GATTAAAGATAAGAGACAGTTGGAC3340.11488757184772926No Hit
CTGAAGGACCTGGAATATGGCGAGA3310.11385564754969575No Hit
ATTTAGAAATGTCCACTGTAGGACG3270.1124797484856511No Hit
ATCGTAAATAGATAGAAACCGACCT3220.11075987465559527No Hit
TTGATATACACTGTTCTACAAATCC3210.11041589988958411No Hit
GTTATAGATTAACCCAATTTTAAGT3190.10972795035756176No Hit
CTATAGAACTAGTACCGCAAGGGAA3160.10869602605952826No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3150.1083520512935171No Hit
CATTTACACCTACTACCCAACTATC3150.1083520512935171No Hit
GTAGAACATATTAGATGAGTGAGTT3110.10697615222947245No Hit
GTATCCTGACCGTGCAAAGGTAGCA3070.10560025316542777No Hit
GAATATGGCAAGAAAACTGAAAATC3010.1035364045693608No Hit
CTGTAGGACATGGAATATGGCAAGA2990.10284845503733846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTGA307.6964364E-419.00206812
GCTAATG307.73219E-418.9889831
AGGGGTC453.5196288E-516.8907265
CCAATTT907.2759576E-1216.88781714
TAGAAAT1001.8189894E-1216.1461944
CCAACGA651.9026993E-716.07313519
TTTAGAA951.8189894E-1115.9879692
ATTTAGA901.3460522E-1015.8241521
ACCCAAT851.0040822E-915.64876212
CCCAATT851.0077201E-915.64606613
TAGATAG551.1172013E-515.5525059
TCCAACG953.0195224E-1014.99646618
GAAGATA456.742169E-414.7768416
AGATAAT456.7509833E-414.77429618
ATAGATT902.2664608E-914.7742964
TCTTAAA456.777485E-414.7666662
TATAGGG456.777485E-414.7666662
TTGTAGA2200.014.67330551
AGATAGA653.3336219E-614.62201310
TAGGACC3200.014.5434474
Produced by FastQC (version 0.11.5)