##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062024_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290719
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25995892941294	32.0	32.0	32.0	32.0	32.0
2	30.955156009755125	32.0	32.0	32.0	32.0	32.0
3	31.00283091232427	32.0	32.0	32.0	32.0	32.0
4	30.99568999618188	32.0	32.0	32.0	32.0	32.0
5	31.002548853016144	32.0	32.0	32.0	32.0	32.0
6	34.62306557190964	36.0	36.0	36.0	32.0	36.0
7	34.53180562673922	36.0	36.0	36.0	32.0	36.0
8	34.494955610056444	36.0	36.0	36.0	32.0	36.0
9	34.542596115148996	36.0	36.0	36.0	32.0	36.0
10	34.361706665199044	36.0	36.0	36.0	32.0	36.0
11	34.57750955389913	36.0	36.0	36.0	32.0	36.0
12	34.425001461892755	36.0	36.0	36.0	32.0	36.0
13	34.516385237978945	36.0	36.0	36.0	32.0	36.0
14	34.46493005273133	36.0	36.0	36.0	32.0	36.0
15	34.41137318166339	36.0	36.0	36.0	32.0	36.0
16	34.44358297875268	36.0	36.0	36.0	32.0	36.0
17	34.375145759307095	36.0	36.0	36.0	32.0	36.0
18	34.42660782405003	36.0	36.0	36.0	32.0	36.0
19	34.31339196956511	36.0	36.0	36.0	32.0	36.0
20	34.259508322469465	36.0	36.0	36.0	32.0	36.0
21	34.216449561260184	36.0	36.0	36.0	32.0	36.0
22	34.18142949033259	36.0	36.0	36.0	32.0	36.0
23	34.17278540446273	36.0	36.0	36.0	32.0	36.0
24	34.14404287301483	36.0	36.0	36.0	32.0	36.0
25	33.842552430353706	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	20.0
6	50.0
7	14.0
8	49.0
9	44.0
10	20.0
11	7.0
12	9.0
13	12.0
14	62.0
15	86.0
16	132.0
17	169.0
18	222.0
19	282.0
20	411.0
21	581.0
22	796.0
23	1055.0
24	1451.0
25	1935.0
26	2748.0
27	3526.0
28	4670.0
29	6300.0
30	8492.0
31	11839.0
32	17395.0
33	25660.0
34	60561.0
35	142119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.730268475998834	22.04186230500662	13.292399772973532	23.935469446021017
2	12.568193231929223	22.589244560020365	44.71756134811054	20.125000859939874
3	18.268499822852995	27.211155789611276	31.56897210020673	22.951372287329004
4	9.641277201769533	19.493770166977413	42.52832836827223	28.336624262980827
5	9.8573812513978	41.74824092074251	37.34822027629156	11.046157551568118
6	26.705650337808766	43.12491180489349	19.223951897958692	10.945485959339045
7	24.61719102400325	34.49120064458592	22.428284650797643	18.463323680613183
8	22.105071053981742	41.169988946775796	20.750104162003765	15.974835837238691
9	24.848912687890632	16.221353673444792	21.87021126396804	37.059522374696535
10	14.18573573780429	29.65820325953683	35.210850427762544	20.94521057489633
11	32.11343188988854	23.79238368167978	27.669775836651468	16.424408591780217
12	21.784190205242837	27.696244734603344	33.56283516854436	16.956729891609463
13	27.29934046253724	23.189199428286063	28.583974789481843	20.927485319694856
14	18.841188563400262	26.292953536661372	28.508510645624497	26.35734725431386
15	21.801981152871157	34.831712223079464	24.36418494434035	19.002121679709028
16	19.698404553891503	29.535898398355307	31.412691252079107	19.35300579567408
17	17.409931706174685	31.10485840138034	30.185319754927452	21.299890137517522
18	17.288303674728663	30.734188198997302	33.91032174535839	18.06718638091565
19	21.911667492976367	28.22570649479425	28.35722745551699	21.50539855671239
20	20.62356477162009	31.71876237265845	29.87237441428911	17.785298441432353
21	21.64757157217808	28.130379101887936	27.810559220038833	22.41149010589515
22	20.76926255190508	31.200203832884583	28.915347376684547	19.11518623852579
23	21.031651778798988	30.630109636667186	29.068690728214662	19.269547856319168
24	21.711826476157686	30.010673093475642	29.65398519538647	18.623515234980204
25	20.740322514011872	30.35480672569784	30.999283914234958	17.905586846055332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	686.0
1	686.0
2	458.5
3	231.0
4	231.0
5	231.0
6	598.5
7	966.0
8	966.0
9	966.0
10	1002.0
11	1038.0
12	1038.0
13	1038.0
14	1384.5
15	1731.0
16	1731.0
17	1731.0
18	3423.5
19	5116.0
20	5116.0
21	5116.0
22	7905.5
23	10695.0
24	10695.0
25	10695.0
26	17290.5
27	23886.0
28	23886.0
29	23886.0
30	31132.5
31	38379.0
32	38379.0
33	38379.0
34	38636.0
35	38893.0
36	38893.0
37	38893.0
38	37787.0
39	36681.0
40	36681.0
41	36681.0
42	34967.5
43	33254.0
44	33254.0
45	33254.0
46	34039.0
47	34824.0
48	34824.0
49	34824.0
50	30290.0
51	25756.0
52	25756.0
53	25756.0
54	20797.0
55	15838.0
56	15838.0
57	15838.0
58	12873.5
59	9909.0
60	9909.0
61	9909.0
62	7858.0
63	5807.0
64	5807.0
65	5807.0
66	4727.0
67	3647.0
68	3647.0
69	3647.0
70	2737.5
71	1828.0
72	1828.0
73	1828.0
74	1406.5
75	985.0
76	985.0
77	985.0
78	665.0
79	345.0
80	345.0
81	345.0
82	211.5
83	78.0
84	78.0
85	78.0
86	52.0
87	26.0
88	26.0
89	26.0
90	21.0
91	16.0
92	16.0
93	16.0
94	14.0
95	12.0
96	12.0
97	12.0
98	52.0
99	92.0
100	92.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013758990640446616
2	3.439747660111654E-4
3	0.0
4	0.007223470086234474
5	0.028893880344937894
6	0.05847571022189812
7	0.10319242980334964
8	0.10525627839941662
9	0.11144782418761759
10	0.12761463819014238
11	0.13587003257441033
12	0.13071041108424286
13	0.12520681482806423
14	0.10938397559155061
15	0.13174233538227634
16	0.1135116727836846
17	0.1224550166999749
18	0.10422435410138312
19	0.09321716158902582
20	0.08943343916290301
21	0.0835858681407132
22	0.09803280831318215
23	0.0736105999263894
24	0.0925292120570035
25	0.08633766626880253
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	290719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.062641244638296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.75267150213166	20.735142869919063
2	14.927191185427164	9.273559691661019
3	6.2233541885831345	5.799414554948249
4	2.9810088034992526	3.703920280408229
5	1.8382149382647694	2.854990557892673
6	1.1992691434582803	2.235148029540553
7	0.8637395493051326	1.8781022224209631
8	0.6655223963235701	1.6538306749816833
9	0.4828082608936382	1.3497569818278132
>10	3.395160843807098	21.231842432039187
>50	0.3853607219976745	8.313870094489868
>100	0.264658656774265	15.643628383421792
>500	0.01771773434472067	4.099147286555058
>1k	0.0033220751896351256	1.2276459398938493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCG	1294	0.4451033472184481	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1184	0.40726612295721987	No Hit
TTGTAGAACAGTGTATATCAATGAG	1091	0.3752764697181815	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	960	0.33021577537071883	No Hit
GTGTATATCAATGAGTTACAATGAA	937	0.32230435575246197	No Hit
CTGTAGGACGTGGAATATGGCAAGA	904	0.31095318847409353	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	896	0.30820139034600424	No Hit
GTCCTACAGTGGACATTTCTAAATT	895	0.307857415579993	No Hit
CTTTAGGACGTGAAATATGGCGAGG	826	0.28412315672522265	No Hit
TATCAACGCAGAGTACTTTTTTTTT	776	0.2669244184246644	No Hit
GTCCTACAGTGTGCATTTCTCATTT	741	0.2548853016142736	No Hit
GTGTATATCAATGAGTTACAATGAG	733	0.25213350348618424	No Hit
CTGTAGGACCTGGAATATGGCGAGA	707	0.24319015956989395	No Hit
GTTCTACAAATCCCGTTTCCAACGA	669	0.23011911846146965	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	621	0.21360832969293375	No Hit
GTTCTACAGTGTGGTTTTTATCATT	591	0.20328908671259877	No Hit
GATATACACTGTTCTACAATGCCGG	588	0.20225716241456526	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	568	0.19537766709434196	No Hit
GGTATCAACGCAGAGTACTTTTTTT	505	0.17370725683563853	No Hit
GTCAGGATACCGCGGCCGTTAAACT	465	0.15994826619519192	No Hit
ACCTATAACTTCTCTGTTAACCCAA	463	0.1592603166631696	No Hit
CTGTAGAACATATTAGATGAGTGAG	451	0.15513261947103563	No Hit
ATAAATAATCCACCTATAACTTCTC	429	0.14756517461878996	No Hit
CTCTAATACTTGTAATGCTAGAGGT	425	0.14618927555474528	No Hit
CCTATAACTTCTCTGTTAACCCAAC	417	0.14343747742665597	No Hit
CCATTGGGATGTCCTGATCCAACAT	409	0.14068567929856668	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	409	0.14068567929856668	No Hit
GTTATATAATTTAAGCTCCATAGGG	405	0.139309780234522	No Hit
CTGTTAGTATGAGTAACAAGAATTC	401	0.13793388117047733	No Hit
CTTCTACACCATTGGGATGTCCTGA	398	0.13690195687244383	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	397	0.1365579821064327	No Hit
GACTATAGGCAATAATCACACTATA	393	0.13518208304238802	No Hit
CACTATAAATAATCCACCTATAACT	390	0.13415015874435451	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	381	0.13105438585025403	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	379	0.1303664363182317	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	376	0.12933451202019822	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	368	0.12658271389210887	No Hit
GTATCAACGCAGAGTACATGGGAGA	365	0.12555078959407537	No Hit
GTACATGGGAGAAATCGTAAATAGA	363	0.12486284006205306	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	360	0.12383091576401956	No Hit
CCTCTAGCATTACAAGTATTAGAGG	353	0.1214230924019414	No Hit
CTGTTAACCCAACACCGGAATGCCT	352	0.12107911763593023	No Hit
GTAGAACAGTGTATATCAATGAGTT	352	0.12107911763593023	No Hit
GATTAAACCTTGTACCTTTTGCATA	352	0.12107911763593023	No Hit
ATCTATAACTTTATAGATGCAACAC	340	0.11695142044379624	No Hit
GATTAAAGATAAGAGACAGTTGGAC	334	0.11488757184772926	No Hit
CTGAAGGACCTGGAATATGGCGAGA	331	0.11385564754969575	No Hit
ATTTAGAAATGTCCACTGTAGGACG	327	0.1124797484856511	No Hit
ATCGTAAATAGATAGAAACCGACCT	322	0.11075987465559527	No Hit
TTGATATACACTGTTCTACAAATCC	321	0.11041589988958411	No Hit
GTTATAGATTAACCCAATTTTAAGT	319	0.10972795035756176	No Hit
CTATAGAACTAGTACCGCAAGGGAA	316	0.10869602605952826	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	315	0.1083520512935171	No Hit
CATTTACACCTACTACCCAACTATC	315	0.1083520512935171	No Hit
GTAGAACATATTAGATGAGTGAGTT	311	0.10697615222947245	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	307	0.10560025316542777	No Hit
GAATATGGCAAGAAAACTGAAAATC	301	0.1035364045693608	No Hit
CTGTAGGACATGGAATATGGCAAGA	299	0.10284845503733846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.439747660111654E-4	0.0
2	0.0	0.0	0.0	3.439747660111654E-4	0.0
3	0.0	0.0	0.0	3.439747660111654E-4	0.0
4	0.0	0.0	0.0	3.439747660111654E-4	0.0
5	0.0	0.0	0.0	3.439747660111654E-4	0.0
6	0.0	0.0	0.0	3.439747660111654E-4	0.0
7	0.0	0.0	0.0	6.879495320223308E-4	0.0
8	0.0	0.0	0.0	6.879495320223308E-4	0.0
9	0.0	0.0	0.0	0.0010319242980334964	0.0
10	0.0	0.0	0.0	0.0010319242980334964	0.0
11	0.0	0.0	0.0	0.0010319242980334964	0.0
12	0.0	0.0	0.0	0.0010319242980334964	0.0
13	0.0	0.0	0.0	0.0010319242980334964	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTTGA	30	7.6964364E-4	19.002068	12
GCTAATG	30	7.73219E-4	18.988983	1
AGGGGTC	45	3.5196288E-5	16.890726	5
CCAATTT	90	7.2759576E-12	16.887817	14
TAGAAAT	100	1.8189894E-12	16.146194	4
CCAACGA	65	1.9026993E-7	16.073135	19
TTTAGAA	95	1.8189894E-11	15.987969	2
ATTTAGA	90	1.3460522E-10	15.824152	1
ACCCAAT	85	1.0040822E-9	15.648762	12
CCCAATT	85	1.0077201E-9	15.646066	13
TAGATAG	55	1.1172013E-5	15.552505	9
TCCAACG	95	3.0195224E-10	14.996466	18
GAAGATA	45	6.742169E-4	14.77684	16
AGATAAT	45	6.7509833E-4	14.774296	18
ATAGATT	90	2.2664608E-9	14.774296	4
TCTTAAA	45	6.777485E-4	14.766666	2
TATAGGG	45	6.777485E-4	14.766666	2
TTGTAGA	220	0.0	14.6733055	1
AGATAGA	65	3.3336219E-6	14.622013	10
TAGGACC	320	0.0	14.543447	4
>>END_MODULE