FastQCFastQC Report
Thu 2 Feb 2017
SRR4062024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290719
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG8970.3085453651120154No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG7250.24938170535809492No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5800.19950536428647594No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT5370.18471444934799583No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA4600.1582283923651361No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC4600.1582283923651361No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.15100492227890164No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC4390.15100492227890164No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA4380.15066094751289044No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC4300.14790914938480115No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC4280.1472211998527788No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA4280.1472211998527788No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC4260.14653325032075648No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC4220.1451573512567118No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT4190.1441254269586783No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC4010.13793388117047733No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG3960.1362140073404215No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC3820.1313983606162652No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA3800.13071041108424286No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA3770.12967848678620936No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT3750.12899053725418702No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC3660.12589476436008654No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA3630.12486284006205306No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC3610.12417489053003071No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT3420.11763936997581859No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA3340.11488757184772926No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA3300.1135116727836846No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC3300.1135116727836846No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA3280.11282372325166225No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC3280.11282372325166225No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA3200.11007192512357293No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC3190.10972795035756176No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG3160.10869602605952826No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT3150.1083520512935171No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT3140.10800807652750595No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3080.10594422793143894No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3080.10594422793143894No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG2970.10216050550531613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT602.9338844E-729.3316121
TCGGGGT300.005739575729.33161224
ATAAGCA300.005739575729.33161232
GGCATAA300.005739575729.33161214
GTTCGTC407.0216093E-427.49838818
AGGGGTC508.3233695E-526.3984535
CCATTTA508.3233695E-526.39845310
GCGTTTA701.1279026E-625.14138435
TGTAGGA6350.024.943422
CTGTAGG6500.024.7062451
ATGTTCG450.001396140324.4430116
TAGGACC2900.024.2744394
AGGACCT3650.024.1081755
GGGCGTT551.5901947E-423.99859233
GTGTAAG551.5901947E-423.9985921
GGACCTG3650.023.5054726
GTATAGG852.3412395E-723.2927511
CTAGGAC1450.022.7572863
GAACTGG500.002576646221.998717
ACTCTTA500.002576646221.9987119
Produced by FastQC (version 0.11.5)