##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290719
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18241669791104	32.0	32.0	32.0	32.0	32.0
2	31.49967838359378	32.0	32.0	32.0	32.0	32.0
3	31.619433198380566	32.0	32.0	32.0	32.0	32.0
4	31.6974982715268	32.0	32.0	32.0	32.0	32.0
5	31.68553138941727	32.0	32.0	32.0	32.0	32.0
6	35.31891620430725	36.0	36.0	36.0	36.0	36.0
7	35.3279317829244	36.0	36.0	36.0	36.0	36.0
8	35.29160804763362	36.0	36.0	36.0	36.0	36.0
9	35.34476246822533	36.0	36.0	36.0	36.0	36.0
10	35.26352250798882	36.0	36.0	36.0	36.0	36.0
11	35.37251091259945	36.0	36.0	36.0	36.0	36.0
12	35.29025278705554	36.0	36.0	36.0	36.0	36.0
13	35.33159511418242	36.0	36.0	36.0	36.0	36.0
14	35.30430759599476	36.0	36.0	36.0	36.0	36.0
15	35.292457665305676	36.0	36.0	36.0	36.0	36.0
16	35.31335069259319	36.0	36.0	36.0	36.0	36.0
17	35.30127373855854	36.0	36.0	36.0	36.0	36.0
18	35.300651144232056	36.0	36.0	36.0	36.0	36.0
19	35.23704333050127	36.0	36.0	36.0	36.0	36.0
20	35.20907818202457	36.0	36.0	36.0	36.0	36.0
21	35.177817755289475	36.0	36.0	36.0	36.0	36.0
22	35.158500132430284	36.0	36.0	36.0	36.0	36.0
23	35.14883788125303	36.0	36.0	36.0	36.0	36.0
24	35.13420863445457	36.0	36.0	36.0	36.0	36.0
25	35.10422779384904	36.0	36.0	36.0	36.0	36.0
26	35.08990468459234	36.0	36.0	36.0	36.0	36.0
27	35.061217189107005	36.0	36.0	36.0	36.0	36.0
28	35.011536913652016	36.0	36.0	36.0	36.0	36.0
29	34.953119679140336	36.0	36.0	36.0	36.0	36.0
30	34.902218293266	36.0	36.0	36.0	36.0	36.0
31	34.925044458738505	36.0	36.0	36.0	36.0	36.0
32	34.87337256938831	36.0	36.0	36.0	36.0	36.0
33	34.85800377684293	36.0	36.0	36.0	36.0	36.0
34	34.83912300193658	36.0	36.0	36.0	36.0	36.0
35	34.81992233049783	36.0	36.0	36.0	36.0	36.0
36	34.75946876537137	36.0	36.0	36.0	32.0	36.0
37	34.690206006487365	36.0	36.0	36.0	32.0	36.0
38	34.6395316439586	36.0	36.0	36.0	32.0	36.0
39	34.5163370815117	36.0	36.0	36.0	32.0	36.0
40	34.573134194875465	36.0	36.0	36.0	32.0	36.0
41	34.5588764408243	36.0	36.0	36.0	32.0	36.0
42	34.50139137792851	36.0	36.0	36.0	32.0	36.0
43	34.53998190692731	36.0	36.0	36.0	32.0	36.0
44	34.42980334962627	36.0	36.0	36.0	32.0	36.0
45	34.36075385509719	36.0	36.0	36.0	32.0	36.0
46	34.22687887616564	36.0	36.0	36.0	32.0	36.0
47	34.203492031824545	36.0	36.0	36.0	32.0	36.0
48	34.100825883413194	36.0	36.0	36.0	32.0	36.0
49	34.07726017219377	36.0	36.0	36.0	32.0	36.0
50	33.69212194593405	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	6.0
22	7.0
23	36.0
24	124.0
25	311.0
26	708.0
27	1468.0
28	2631.0
29	4347.0
30	6487.0
31	9062.0
32	13320.0
33	21910.0
34	47520.0
35	182777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.376516129032254	22.58374193548387	13.888344086021506	24.151397849462366
2	12.149137290514323	22.9185585734335	44.353174275571696	20.579129860480474
3	17.70478608135719	27.518763888028946	31.987699420056547	22.788750610557308
4	9.581761082007025	19.70184267282152	42.5231924985983	28.193203746573154
5	9.662939126785659	41.782958802142275	37.39865643456396	11.155445636508105
6	26.911553768415548	43.19084751942597	19.040379197782052	10.857219514376425
7	24.6334088931236	34.15497439107867	22.684447868904336	18.527168846893392
8	22.321210515996544	41.01623904870338	20.650181102714306	16.01236933258576
9	24.936450661979435	15.824903085109677	22.143031587202763	37.09561466570812
10	14.068223955090655	29.578734104066122	35.53087345512333	20.82216848571989
11	32.33775570224168	23.46699046157974	27.854732576818165	16.340521259360415
12	21.806968240809855	27.54928298460025	33.7060873214341	16.937661453155798
13	27.38348714738287	23.053532792834318	28.720173088102257	20.84280697168056
14	18.736993454160203	26.14208221684857	28.8570750449747	26.263849284016526
15	21.73679738854358	34.75589830730018	24.46589318207616	19.041411122080085
16	19.847204832207378	29.140810963276877	31.414507629439044	19.59747657507671
17	17.55819193103994	30.820139034600423	30.29144981924126	21.33021921511838
18	17.327728837812458	30.096760101678942	34.430154203887604	18.145356856621
19	22.18362060959208	27.715766771349653	28.728084507720514	21.37252811133775
20	20.639244903996314	31.130511354646085	30.206591955090502	18.023651786267102
21	21.71443902875285	27.616014089206413	28.152614724183834	22.5169321578569
22	20.804625772653317	30.897189382186923	29.154269242808343	19.14391560235141
23	21.098724197592862	30.392578400448546	29.209305205370136	19.299392196588457
24	21.900218083752414	29.87369029355311	29.73025034914039	18.495841273554078
25	21.04665721872893	29.94124850369433	31.11077477675807	17.90131950081867
26	16.904756171809698	31.23931521032482	33.58695913895644	18.268969478909042
27	18.23869949125782	30.993027583526246	31.34560414423649	19.42266878097944
28	18.242139262581823	30.9235442027814	30.288906393502963	20.545410141133818
29	19.22447182493	28.282046519307368	29.259626166938407	23.233855488824222
30	19.009486856678983	29.890822033723403	30.40162631828783	20.698064791309793
31	21.41552495708915	28.093107089663903	30.38088325840416	20.110484694842786
32	18.487675341740108	31.379893917817263	30.4384317448524	19.693998995590228
33	19.561156993522953	30.722106226287238	30.918515817679616	18.79822096251019
34	19.256938042625794	30.571760756201925	32.42477194237675	17.746529258795526
35	19.060673708976708	31.714817400995464	30.500930451742057	18.723578438285767
36	17.67314029403064	30.845699268707133	31.087514360995876	20.393646076266347
37	19.605381289321695	29.788690486428106	28.766369349629013	21.839558874621186
38	20.052352959386898	31.45374055359299	29.259869495973774	19.234036991046334
39	20.497597319730186	29.371519381391526	29.3343698510923	20.79651344778599
40	22.16030655136593	29.93072324383079	30.271259433540408	17.637710771262874
41	20.955791298604804	27.94380692919453	32.787432235339445	18.312969536861225
42	20.191881004881303	32.43126098637432	29.26856116766024	18.108296841084137
43	17.7233652447016	31.947258523362493	31.268554287738947	19.06082194419696
44	17.85211812662321	31.77310331779639	30.037323059458902	20.3374554961215
45	18.184382427909505	31.8285169807816	30.68889722095139	19.298203370357502
46	19.99112514576432	28.891090403117897	28.829516802025395	22.28826764909239
47	21.4013724782003	30.70808179832482	28.33152744096452	19.559018282510362
48	19.20414986859667	30.627296118441873	27.8685141103788	22.30003990258266
49	20.233504528708192	29.944513434766307	30.071103986570392	19.75087804995511
50	18.843949723783513	31.571922920808763	27.959437797973262	21.624689557434454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	381.0
1	202.5
2	24.0
3	71.5
4	119.0
5	113.5
6	108.0
7	73.0
8	38.0
9	83.5
10	129.0
11	189.0
12	249.0
13	511.5
14	774.0
15	987.0
16	1200.0
17	1498.0
18	1796.0
19	1802.0
20	1808.0
21	2181.0
22	2554.0
23	2732.0
24	2910.0
25	4438.0
26	5966.0
27	8977.5
28	11989.0
29	13616.5
30	15244.0
31	15693.5
32	16143.0
33	19308.5
34	22474.0
35	25562.0
36	28650.0
37	30243.5
38	31837.0
39	29813.0
40	27789.0
41	25885.0
42	23981.0
43	19743.5
44	15506.0
45	14605.0
46	13704.0
47	12739.5
48	11775.0
49	10700.5
50	9626.0
51	9332.0
52	9038.0
53	8419.0
54	7800.0
55	7164.5
56	6529.0
57	6246.0
58	5963.0
59	5210.0
60	4457.0
61	3810.0
62	3163.0
63	2749.5
64	2336.0
65	2042.5
66	1749.0
67	1510.0
68	1271.0
69	1080.5
70	890.0
71	614.5
72	339.0
73	263.0
74	187.0
75	144.5
76	102.0
77	87.0
78	72.0
79	47.5
80	23.0
81	15.5
82	8.0
83	8.5
84	9.0
85	6.5
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032333628005049554
2	0.002407823362078158
3	3.439747660111654E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010319242980334964
17	0.0
18	0.0
19	0.0
20	3.439747660111654E-4
21	0.0
22	0.0
23	0.0
24	0.001719873830055827
25	0.0010319242980334964
26	6.879495320223308E-4
27	6.879495320223308E-4
28	6.879495320223308E-4
29	3.439747660111654E-4
30	3.439747660111654E-4
31	0.0
32	3.439747660111654E-4
33	0.0
34	0.0010319242980334964
35	0.0
36	3.439747660111654E-4
37	0.002751798128089323
38	0.0
39	6.879495320223308E-4
40	3.439747660111654E-4
41	0.002407823362078158
42	0.006191545788200978
43	0.006191545788200978
44	0.004815646724156316
45	0.002751798128089323
46	0.003439747660111654
47	0.0013758990640446616
48	0.0037837224261228195
49	0.006191545788200978
50	0.001719873830055827
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	290719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.699147934830194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.73511680406837	23.823868072366096
2	15.814104070177015	11.291000813160991
3	6.225004778560113	6.6668209945452706
4	3.109468142564668	4.4402145288023105
5	1.7354050297127297	3.097624044128157
6	1.1349624416139776	2.431031526219208
7	0.7759205555884277	1.9389791889738839
8	0.5714682198745521	1.6320742817084561
9	0.47273284560551715	1.5188543810032138
>10	2.907207243774881	20.569688766284575
>50	0.31316057441607836	7.8449219760665105
>100	0.20145998736320966	13.769735745864223
>500	0.003989306680459597	0.9751856808771069
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	897	0.3085453651120154	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	725	0.24938170535809492	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	580	0.19950536428647594	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	537	0.18471444934799583	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	460	0.1582283923651361	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	460	0.1582283923651361	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.15100492227890164	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	439	0.15100492227890164	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	438	0.15066094751289044	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	430	0.14790914938480115	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	428	0.1472211998527788	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	428	0.1472211998527788	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	426	0.14653325032075648	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	422	0.1451573512567118	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	419	0.1441254269586783	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	401	0.13793388117047733	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	396	0.1362140073404215	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	382	0.1313983606162652	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	380	0.13071041108424286	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	377	0.12967848678620936	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	375	0.12899053725418702	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	366	0.12589476436008654	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	363	0.12486284006205306	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	361	0.12417489053003071	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	342	0.11763936997581859	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	334	0.11488757184772926	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	330	0.1135116727836846	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	330	0.1135116727836846	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	328	0.11282372325166225	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	328	0.11282372325166225	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	320	0.11007192512357293	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC	319	0.10972795035756176	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	316	0.10869602605952826	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	315	0.1083520512935171	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	314	0.10800807652750595	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	308	0.10594422793143894	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	308	0.10594422793143894	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	297	0.10216050550531613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	3.439747660111654E-4	0.0
18	0.0	0.0	0.0	3.439747660111654E-4	0.0
19	0.0	0.0	0.0	3.439747660111654E-4	0.0
20	0.0	0.0	0.0	0.0010319242980334964	0.0
21	0.0	0.0	0.0	0.0013758990640446616	0.0
22	0.0	0.0	0.0	0.0013758990640446616	0.0
23	0.0	0.0	0.0	0.001719873830055827	0.0
24	0.0	0.0	0.0	0.0030957728941004886	0.0
25	0.0	0.0	0.0	0.003439747660111654	0.0
26	0.0	0.0	0.0	0.0037837224261228195	0.0
27	0.0	0.0	0.0	0.004127697192133985	0.0
28	0.0	0.0	0.0	0.006191545788200977	0.0
29	0.0	0.0	0.0	0.008943343916290302	0.0
30	0.0	0.0	0.0	0.014102965406457783	0.0
31	0.0	0.0	0.0	0.02545413268482624	0.0
32	0.0	0.0	0.0	0.03852517379325053	0.0
33	0.0	0.0	0.0	0.045404669113473836	0.0
34	0.0	0.0	0.0	0.054691987795775304	0.0
35	0.0	0.0	0.0	0.06741905413818843	0.0
36	0.0	0.0	0.0	0.08117804477863504	0.0
37	0.0	0.0	0.0	0.10456832886739428	0.0
38	0.0	0.0	0.0	0.13758990640446617	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	60	2.9338844E-7	29.331612	1
TCGGGGT	30	0.0057395757	29.331612	24
ATAAGCA	30	0.0057395757	29.331612	32
GGCATAA	30	0.0057395757	29.331612	14
GTTCGTC	40	7.0216093E-4	27.498388	18
AGGGGTC	50	8.3233695E-5	26.398453	5
CCATTTA	50	8.3233695E-5	26.398453	10
GCGTTTA	70	1.1279026E-6	25.141384	35
TGTAGGA	635	0.0	24.94342	2
CTGTAGG	650	0.0	24.706245	1
ATGTTCG	45	0.0013961403	24.44301	16
TAGGACC	290	0.0	24.274439	4
AGGACCT	365	0.0	24.108175	5
GGGCGTT	55	1.5901947E-4	23.998592	33
GTGTAAG	55	1.5901947E-4	23.998592	1
GGACCTG	365	0.0	23.505472	6
GTATAGG	85	2.3412395E-7	23.292751	1
CTAGGAC	145	0.0	22.757286	3
GAACTGG	50	0.0025766462	21.99871	7
ACTCTTA	50	0.0025766462	21.99871	19
>>END_MODULE